Abstract:Benzoic acid with weak fluorescence may react on ·OH, and then products with intense fluorescence are made. Extractives of Chinese traditional medicine may eliminate ·OH in solution, and reduce the amounts of the products. Then, the increased level of fluorescence of the products in solution will be lowered. Based on this principle, a novel catalysis kinetics fluorimetry system for the determination of eliminating ratio of Chinese traditional medicine for ·OH was developed. When the concentration of traditional Chinese herbal drugs was 4.0 mg(dry weight )·mL-1, the eliminating ratios of Viola yedoensis, Atrctylodes chinensis and Paeonia veitchii were 60.8%, 40.1% and 94.3%, respectively. The results obtained by this method are in good agreement with those obtained by spectrophotometry.
瞿 鹏,李贯良,徐茂田. 催化动力学荧光法测定中草药对羟基自由基的清除率[J]. 光谱学与光谱分析, 2004, 24(11): 1407-1409.
QU Peng,LI Guan-liang,XU Mao-tian. A Novel Catalysis Kinetics Fluorimetry System for the Determination of Eliminating Ratio of Chinese Traditional Medicine for ·OH . SPECTROSCOPY AND SPECTRAL ANALYSIS, 2004, 24(11): 1407-1409.
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