Abstract：A new method for analyzing the ingredients of a pair of optic isomers in ephedra, nor-ephedrine and nor-pseudoe-ephedrine, using hyphenated high-efficiency thin layer chromatography (TLC) and surface-enhanced Raman spectroscopy (SERS) techniques, is reported. The results show that the characteristic spectral bands of nor-ephedrine and nor-pseudo-ephedrine can be obtained from the TLC spot with 8 μg sample of about 2.0 mm in diameter. The difference between the SERS and solid spectra was found. Spectral bands at 1 004 cm-1 and 1 605 cm-1 were found greatly enhanced. Molecule was absorbed in surface silver sol by π electrons in ring. Under similar experimental conditions the spectral information of Levo-nor-ephedrine ramifications TLC-SERS is rich with strong credibility, whereas dextral-nor-ephedrine ramifications show a relatively strong fluorescence backdrop with less spectral information and weak credibility. The effective combination of TLC and SERS can be used to analyse the chemical ingredients with high sensitivity.
汪 瑗1，张进治2，马新勇1* . 麻黄生物碱中一对光学异构体在薄层色谱原位的表面增强拉曼光谱研究[J]. 光谱学与光谱分析, 2004, 24(11): 1373-1375.
WANG Yuan1，ZHANG Jin-zhi2，MA Xin-yong1 . TLC-FT-SERS Study on a Pair of Optic Isomers in Ephedra . SPECTROSCOPY AND SPECTRAL ANALYSIS, 2004, 24(11): 1373-1375.
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