1. China Astronaut Research and Training Center, Beijing 100094, China 2. College of Science, China Agricultural University, Beijing 100094, China 3. College of Information and Electrical Engineering, China Agricultural University, Beijing 100094, China
Abstract:In this research, The functional components of vegetable oils were analyzed by near infrared(NIR) spectral technology. The optimum conditions of mathematics model of four components(C16:0,C18:0,C18:1,C18:2)were studied, including the sample set selection, chemical value analysis, the detection methods and condition. Chemical value was analyzed by HPLC. 52 samples were selected, 41 for modeling set and 11 for testing set. All samples were placed in 5mm thick sample pools and swept by near infrared(NIR) with discrimination factor 8 cm-1 without any other disposal. Using PLS methods sated model. Data were processed by first derivative method and centering method. 5 000-9 000 cm-1 spectral region was analyzed. Correlating index (r), RMSECV and RMSEP were chose as evaluation index. The result demonstrated that the correlation between the reference value of the modeling sample set and the near infrared predictive value were r(C16:0)=0.891, r(C18:0)=0.837, r(C18:1)=0.982, r(C18:2)=0.971, respectively. And the correlation between the reference value of the testing sample set and the near infrared predictive value were 0.921, 0.891, 0.946 and 0.949, respectively. It proved that the near infrared predictive value was linear with chemical value and the mathematical model established for components of vegetable oils was feasible. For validation, 8 unknown samples were selected to be analysis by infrared(NIR). The result demonstrated that error between predict value and chemical value was less than 10%. That was to say infrared (NIR) had a good veracity in analysis components of vegetable oil. Because infrared(NIR) spectral technology is convenient, rapid than HPLC in oil components analysis, moreover, infrared(NIR) can analyze many components at the same time. It must have great application prospect in vegetable oil components analysis.
[1] YAN Yan-lu, ZHAO Long-lian, HAN Dong-hai,et al(严衍禄,赵龙莲,韩东海,等). Basis and Aapplication of Near Infrared Reflectance Spectroscopy(近红外光谱分析基础与应用). Beijing:China Light Industry Press(北京:中国轻工业出版社), 2005. 12. [2] LIU Ai-qiu, DENG Xiao-jian, WANG Ping-rong, et al(刘爱秋,邓晓建,王平荣,等). Southwest China Journal of Agriculture Science(西南农业学报),2003, 16(20):98. [3] YAN Yan-lu, ZHAO Long-lian, LI Jun-hui, et al(严衍禄,赵龙莲,李军会,等). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2000, 20(6):777. [4] Cozzolino D, Murray I, Chree A, et al. LWT-Food Science and Technology, 2005, 38(8):821. [5] Man Y B Che, Moh M H. Journal of American Oil Chemists Society, 1998, 75(5):557. [6] Sato T. Lipid- Technology,1997,9(2):486. [7] Li Hui, van de Voort F R, Ismail A A, et al. Journal of American Oil Chemists Society, 2000, 77(1):29. [8] ZHAO Wu-shan, CHEN Yun-bo, LI Xiang-yang, et al (赵武善,陈云波,李向阳, 等). China Oils and Fats(中国油脂), 2003, 28(9):38. [9] Li Hui, van de Voort F R, Ismail A A, et al. Journal of American Oil Chemists Society, 2000, 77(2):137. [10] Voort F R vandc, Memon K. P. Journal of the American Oil Chemists Society, 1996, 73(4):411. [11] WU Jian-guo, SHI Chun-hai, ZHANG Hai-zhen(吴建国,石春海,张海珍). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2006, 26(2):259. [12] YANG Cui-ling, CHEN Wen-jie, ZHANG Wen-xue, et al(杨翠玲,陈文杰,张文学,等). Acta Agriculturae Boreali-Occidentalis Sinica(西北农业学报), 2005, 14(6):72. [13] Tetsuo Sa To. Bioscience, Biotechnology and Biochemistry, 2002, 66(12):2543. [14] XU Yong-qun, TANG Jun-ming(徐永群, 汤俊明). Henan Sciences(河南科学), 2002, 20(3):245. [15] SU Yue, GUO Yin-long(苏 越,郭寅龙). Computers and Applied Chemistry(计算机与应用化学),2001, 18(3):237. [16] CHEN Bin, ZHAO Long-lian, LI Jun-hui, et al(陈 斌,赵龙莲,李军会,等). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2002, 22(16):976. [17] MIN Shun-geng, LI Ning, ZHANG Ming-xiang (闵顺耕,李 宁,张明祥). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2004, 24(10):1205. [18] WANG Tao, ZHANG Lu-da, LAO Cai-lian, et al(王 韬,张录达,劳采莲,等). Journal of China Agricultural University(中国农业大学学报), 2004, 9(6):76. [19] ZHU Shi-ping, WANG Yi-ming, ZHANG Xiao-chao, et al(祝诗平,王一鸣,张小超,等). Transaction of the Chinese Society for Agriculture Machinery(农业机械学报), 2004, 35(4):115. [20] ZHANG Jun, ZHENG Yong-mei, WANG Fang-rong, et al(张 军,郑咏梅,王芳荣,等). Journal of Jilin University(Information Science Edication)(吉林大学学报·信息科学版), 2003, 21(1):4.