Determination of Antibiotics Residues in the Raw Fresh Milk of Farms in the Miyun County of Beijing with Mg2+-Sensitized Metacycline Fluorescence Microscopic Imaging Technique
LIU Ying1, 2, YANG Le1
1. College of Life and Environmental Science, Minzu University of China, Beijing 100081, China 2. College of Chemistry and Environmental Science, Inner Mongolia Normal University, Huhhot 010022, China
Abstract：Mg2+-sensitized metacycline fluorescence microscopic imaging technique was applied to detect the raw fresh milk of four cows breeding farms in the Miyun County of Beijing based on the capillary effect of solvent on solid supports. In the presence of NH3-NH4Cl buffer solution (pH 9.99) and PVA-124, Mg2+ and metacycline can form a strong fluorescence complex of 1∶1, and Mg2+-metacycline complex can form an SOR on the hydrophobic supports with the diameter of 0.93 mm and its ring belt width of 26.2 μm. By measuring the fluorescence intensity of the ring, the quantitative analysis of metacycline was achieved with the detection limit (3σ) of 8.8×10-14 mol·ring-1 (1.8×10-7 mol·L-1) and linear range of 2.2×10-13-3.6×10-12 mol·ring-1 (4.4×10-7-7.2×10-6 mol·L-1) when 0.50 μL droplet was spotted. This method has been satisfactorily applied to the determination of metacycline in the raw fresh milk samples with the recovery of 93.8%-108% and RSD less than 4.3%.
刘 颖1, 2，杨 乐1 . 镁敏化美他环素荧光显微成像技术应用于北京市密云县养殖场生鲜乳抗生素残留量的检测 [J]. 光谱学与光谱分析, 2010, 30(05): 1279-1284.
LIU Ying1, 2, YANG Le1. Determination of Antibiotics Residues in the Raw Fresh Milk of Farms in the Miyun County of Beijing with Mg2+-Sensitized Metacycline Fluorescence Microscopic Imaging Technique . SPECTROSCOPY AND SPECTRAL ANALYSIS, 2010, 30(05): 1279-1284.
[1] Senturk S, Temizel M. Veterinary Record, 2006, 159: 216. [2] YU Jian-liang, LI Kai-xiong, HE Jia-liang, et al(于见亮, 李开雄, 贺家亮, 等). Food and Nutrition in China(中国食物与营养), 2006, (10): 10. [3] GUO Jun, LIU Yan-hui, LI Cui-zhi(郭 军, 刘艳辉, 李翠枝). China Dairy Industry(中国乳品工业), 2004, 32(9): 28. [4] SI Qin, AODENG Gaowa, LI Hu-dong(斯 琴, 敖登高娃, 李虎东). Acta Scientiarum Naturalium Universitatis NeiMongol(内蒙古大学学报·自然科学版), 2007, 38(1): 28. [5] WANG Lei, XU Zhi-xiu, ZHANG Xiao-song, et al(王 蕾, 徐智秀, 张孝松, 等). Chinese Journal of Analytical Chemistry(分析化学), 2003, 31(1): 52. [6] Ragazzi E, Veronese G. J Chromatogr., 1977, 134: 223. [7] XIE Li-qi, YUE Zhen-feng, TANG Shao-bing, et al(谢丽琪, 岳振峰, 唐少冰, 等). Chinese Journal of Analytical Laboratory(分析试验室), 2008, 27(3): 5. [8] JIANG Hong, LIU Shao-pu, HU Xiao-li, et al(江 虹, 刘绍璞, 胡小莉, 等). Chinese Journal of Analytical Chemistry(分析化学), 2003, 31(10): 1207. [9] Thao D T. Electrophoresis, 2006, 27: 2317. [10] Hane M R, Visser H, Koolwijk P, et al. Adv. Dent Res., 1998, 12(2): 114. [11] LIU Ying, HUANG Cheng-zhi, LI Yuan-fang. Anal. Chem., 2002, 74: 5564. [12] HUANG Cheng-zhi, LIU Ying, LI Yuan-fang. J. Pharm. Biomed. Anal., 2004, 34: 103. [13] LIU Ying, LI Yuan-fang, HUANG Cheng-zhi. Journal of Analytical Chemistry, 2006, 61(7): 647.
