Abstract:A new method using single reflection Fourier transform infrared spectroscopy was proposed for direct and fast determination of Corydalis yanhusuo W. T. Wang of traditional Chinese herbal medicines and its confusable varieties. Data were collected directly for Fourier transform infrared spectra with OMNI sampler. Then through converting FTIR spectra of the samples into second derivative spectra by derivative spectra software, it is possible to identify Corydalis yanhusuo W. T. Wang from the confusable varieties with statistics. The result shows that the second derivative FTIR of Corydalis yanhusuo W. T. Wang and its confusable varieties are different in the 2 050-650 cm-1 range. The probability is less than 0.01 and the result is significant. Corydalis yanhusuo W. T. Wang and its confusable varieties can be identified by identifying the inner layer parts of the cuticles of samples by second derivative FTIR spectroscopy with statistics directly, rapidly and accurately.
Key words:Corydalis yanhusuo W. T. Wang;Confusable varieties;Identification;Second derivative FTIR
程存归,应桃开. 导数FTIR结合统计学法应用于中药延胡索质量控制的研究 [J]. 光谱学与光谱分析, 2005, 25(01): 36-38.
CHENG Cun-gui,YING Tao-kai. Quality Control of Corydalis Yanhusuo W. T. Wang by Second Derivative FTIR Spectroscopy Combined with Statistics . SPECTROSCOPY AND SPECTRAL ANALYSIS, 2005, 25(01): 36-38.
[1] National Institute for the Control of Pharmaceutical and Biological Products, State Drug Administrate, P. R. China; Guangdong Provincial Institute for Drug Control(中国药品生物制品检定所,广东省药品检定所). Photo Album for Traditional Chinese Herbal Medicines and Their Confusabe Varieties Identification(中国中药材真伪鉴别图典Ⅱ). Guangzhou: Guangdong Science and Technology Press(广州:广东科技出版社),1997. 110. [2] CAO Gu-zhen, NI Ping(曹谷珍,倪 萍). Chinese Traditional and Herbal Drugs(中草药),1997,28(10):626. [3] ZHANG Ji-qing, YOU Qing-hu,HU Yan-ni(张继庆,尤庆虎,胡燕妮). Chinese Traditional and Herbal Drugs(中草药),2000,31(9):709. [4] CHENG Cun-gui(程存归). Spectroscopy and Spectral Analysis(光谱学与光谱分析),2003,23(2):282 [5] WANG Zhao, SUN Su-qin, LI Xiao-bo,ZHOU Qun, LIN Lin, DU De-guo(王 钊,孙素琴,李晓波, 周 群,林 琳,杜德国). Spectroscopy and Spectral Analysis(光谱学与光谱分析),2001,21(3):311. [6] CHENG Cun-gui, GUO Shui-liang, CHEN Jian-hua(程存归,郭水良,陈建华). Spectroscopy and Spectral Analysis(光谱学与光谱分析),2002,22(6):954.