Abstract:The modifier of quantum dots plays an important role in synthesis and nature of quantum dots, however the effect on the interaction between quantum dots and protein is not very clear until up to now. In the present paper, the interactions of CdTe quantum dots with bovine serum album (BSA) were studied by spectroscopy methods including ultraviolet-visible absorption spectrometry (UV-Vis), fluorescence spectrometry (FL) and infrared spectrometry (IR). The CdTe quantum dots were modified by three different thiol-complex including thioglycolic acid, L-cysteine and glutathione, i.e. thioglycolic acid capped CdTe quantum dots (CdTeT), L-cysteine capped CdTe quantum dots (CdTeL) and glutathione capped CdTe quantum dots (CdTeG) respectively. The quenching constant KSV and corresponding thermodynamic parameters, such as enthalpy change (ΔHθ), entropy change (ΔSθ), Gibbs free energy change (ΔGθ), were calculated according to Stern-Volmer equations. The results showed that CdTeT, CdTeL and CdTeG all have a strong ability of quenching the fluorescence of bovine serum albumin, and the interactions of the three types of thiol-capped CdTe quantum dots with BSA were static quenching process. The quenching constant of KSV (TGA) is similar to KSV (GSH), which is much less than KSV (L-Cys). The binding forces of CdTeT and CdTeL with the BSA were the main contributions from hydrophobic force according to the thermodynamic parameters (ΔHθ>0, ΔSθ>0 and ΔGθ<0), while the binding forces of CdTeG with BSA were composed of both hydrogen bonding force and hydrophobic force according to the thermodynamic parameters(ΔHθ<0, ΔSθ>0 and ΔGθ<0). It was found that different functional group and molecular volume size of thiol surface modified reagent played an important role in the interactions between CdTe QDs and BSA.
马金杰,梁建功,韩鹤友* . 不同巯基试剂修饰的CdTe量子点与BSA相互作用研究 [J]. 光谱学与光谱分析, 2010, 30(04): 1039-1043.
MA Jin-jie, LIANG Jian-gong, HAN He-you* . Study on the Synchronous Interactions between Different Thiol-Capped CdTe Quantum Dots and BSA . SPECTROSCOPY AND SPECTRAL ANALYSIS, 2010, 30(04): 1039-1043.
[1] Bruchez M J, Moronne M, Gin P, et al. Science, 1998, 281: 2013. [2] Jaiswal J K, Mattoussi H, Mauro J M, et al. Nature Biotechnology, 2003, 21(1): 47. [3] Zhang C Y, Yeh H C, Kuroki M T, et al. Nature Materials, 2005, 4(11): 826. [4] LI Rui, DAI Ben-cai, ZHAO Yong-de, et al(李 锐, 代本才, 赵永德, 等). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2009, 29(1): 240. [5] Shao L W, Dong C Q, Sang F M, et al. Journal of Fluorescence, 2009, 19(1): 151. [6] Wu Y L, He F, He X W, et al. Spectrochimica Acta Part A-Molecular and Biomolecular Spectroscopy, 2008, 71 (4): 1199. [7] Xu Q, Wang J H, Wang Z, et al. Electrochemistry Communications, 2008, 10 (9): 1337. [8] Idowu M, Lamprecht E, Nyokong T. Journal of Photochemistry and Photobiology A-Chemistry, 2008, 198(1): 7. [9] ZHANG Ai-mei, YAN Wei, WANG Huai-sheng(张爱梅, 闫 炜, 王怀生). Chinese Journal of Analytical Chemistry(分析化学), 2008, 36(4): 444. [10] CHEN Qi-fan, WANG Wen-xing, GE Ying-xin, et al(陈启凡, 王文星, 葛颖新, 等). Chinese Journal of Analytical Chemistry(分析化学), 2007, 35(1): 135. [11] LI Rui, REN Hai-ping, SUN Yan-ting, et al(李 锐, 任海平, 孙艳亭, 等). Chinese Journal of Analytical Chemistry(分析化学), 2006, 34(12): 1801. [12] Liang J G, Cheng Y P, Han H Y. Journal of Molecular Structure, 2008, 892(1-3): 116. [13] Bao H F, Wang E K, Dong S J. Small, 2006, 2(4): 476. [14] Rogach A L, Franzl T, Klar T A, et al. Journal of Physical Chemistry C, 2007, 111(40): 14628. [15] Jiang C, Xu S K, Yang D Z, et al. Luminescence, 2007, 22: 430. [16] Yu W W, Qu L H, Guo W Z, et al. Chemistry of Materials, 2003, 15(14): 2854. [17] Ross P D, Subramanian S. Biochemistry, 1981, 20(11): 3096. [18] ZHANG Bao-lin, WANG Wen-qing, BAI Feng-lian(张保林, 王文清, 白凤莲). Chemical Journal of Chinese Universites(高等学校化学学报), 1994, 15(3): 373. [19] Peters T. Advances in Protein Chemistry, 1985, 37: 161.