Raman Spectral Study of Nitrosyhemoglobin and Several Other Hemoglobins
CEN Yan1,2, ZHANG Ren3, YAO Wen-hua3, MA Jiong1,2, CHEN Ji-yao1, 4*
1. Department of Physics, Fudan University, Shanghai 200433, China 2. State Key Laboratory of Applied Surface Physics, Fudan University, Ｓhanghai 200433, China 3. Analysis and Measurement Center, Fudan University, Ｓhanghai 200433, China 4. State Key Laboratory for Advanced Photonic Materials and Devices, Fudan University, Ｓhanghai 200433, China
Abstract：The Raman spectra of nitrosyhemoglobin(NOHb), methemoglobin(MetHb), oxygenated hemoglobin(oxyHb)，and deoxygenated hemoglobin(deoxyHb) were comparatively studied. The results show that the Raman spectra with 632.8 nm excitation can be used to detect the R state (oxyHb) to T state (deoxyHb) transition. The difference of NOHb and MetHb in their electron spin state was clearly demonstrated in their Raman spectra with 632.8 nm excitation, suggesting that Raman spectra provide a new simple way to measure and distinguish NOHb and MetHb.
[1] Friedman Joel M. Science, 1985, 228: 1273. [2] Calotta E, Koshland D E. Science, 1992, 258: 1862. [3] Yamada T, Yukioka H, Hayashi M et al. Acta Anaesthesiologica Scandinavica, 1998, 42: 358. [4] Cantilena L R, Smith R P, Frasur S et al. J. Laboratory and Clinical Medical, 1992, 120: 902. [5] Venkatesh B, Ramasamy S, Myljan M et al. Spectrochimica Acta Part A，1999, 55: 1691. [6] Vladimivov Y, Borisenko G, Boriskina N et al. J. Photochem. Photobiol.: B, 2000, 59: 115. [7] Zinchuk V V, Dorokhina L V, Nitric Oxide: Biology and Chemistry, 2002, 6: 29. [8] Perutz M F. Nature, 1970, 228: 726. [9] Wood B R, Tait B, McNaughton D. Bichimica et Biophysica Acta，2001, 1539: 58. [10] Cho N, Song S, Asher S A. Biochemistry, 1994, 33: 5932. [11] Nagai K, Welborn C, Dolphin D et al. Biochemistry, 1980, 19: 4755. [12] Hu S, Smith K M et al. J. Am. Chem. Soc., 1996, 118: 12638.
