Determination of Serum Copper and Zinc in Different Chemical Forms by Graphite Furnace Atomic Absorption Spectrometry with Ethanol-EDTA Precipitation Method
ZHANG Yuan, LIN Zhe-xuan，LI Hui，PENG Lin, LUO Hong-jun，LI Wei-qiu，LUO Wen-hong*
Central Laboratories, Medical College of Shantou University, Shantou 515041, China
Abstract：A simple and reliable method was developed for the determination of serum copper and zinc in different chemical forms by graphite furnace atomic absorption spectrometry (GFAAS) with ethanol-EDTA precipitation. The serum and ethanol were mixed with volume ratio 1∶2. The mixture was incubated at 70 ℃ and ultra-centrifuged to precipitate proteins. Zinc and copper can be released from albumin after EDTA treating. Thus, macroglobulin-zinc, ceruloplasmin-copper, and albumin-bound zinc or copper could be determined by two proposed precipitation steps. The determination limit of copper (3σ) was 1.2 μg·L-1 and the recovery was 92.3%-104%, while the determination limit of zinc (3σ) was 0.098 μg·L-1, and the recovery was 90%-107%. This two-step precipitation method can be used to determine serum zinc and copper in various chemical forms in tumor, Wilson patients and heathy human.
张 源，林哲绚，李 慧，彭 琳，罗红军，李伟秋，罗文鸿* . GFAAS测定乙醇-EDTA法分离血清中不同形态的铜和锌[J]. 光谱学与光谱分析, 2010, 30(03): 816-819.
ZHANG Yuan, LIN Zhe-xuan，LI Hui，PENG Lin, LUO Hong-jun，LI Wei-qiu，LUO Wen-hong* . Determination of Serum Copper and Zinc in Different Chemical Forms by Graphite Furnace Atomic Absorption Spectrometry with Ethanol-EDTA Precipitation Method . SPECTROSCOPY AND SPECTRAL ANALYSIS, 2010, 30(03): 816-819.
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