The Interaction of Vitamin B6 with the Human Serum Albumin
WANG Jian-lin2, FU Lian-chun3, ZHOU Shi-wu4, CHEN Zhi-jian1, Lü Wen-bo1, YE Xue-min1, MENG Guang-zheng5, SONG Zeng-fu1*
1. School of physics, Peking University, Beijing 100871, China 2. College of Material Science and Chemical Engineering, Zhengzhou University of Light Industry, Zhengzhou 450002, China 3. CTA-Tex Chemicals, Beijing 100025, China 4. College of Physical Science and Technology, Yangzhou University, Yangzhou 225002, China 5. Central Iron and Steel Research Institute, Beijing 100081, China
Abstract：The Human Serum Albumin(HSA)can emit fluorescence(λem=350 nm)under irradiation of ultraviolet light(λex=296 nm). After the vitamin B6(B6)was added into HSA solution the fluorescence of HSA was quenched partially. This quenching was static quenching owing to the fact that τ０(fluorescence life time (life time of the molecule excitation state) of HSA without B6) equaled to τi(fluorescence life time of HSA with B6). The formation constant K between HSA and B6 was observed from the experiment, and then the binding distance R０ was determined(R０=1.872 nm). The CD spectra of samples (HSA，=1×10-5 mol·L-1，HSA+B6，[B6]=5×10-8，1.5×10-7，2.5×10-7，3.5×10-7，4.5×10-7 mol·L-1) were measured, and all CD spectra were almost the same. From [θ]values, the contents(percent content)of four structure(α-Helix，β-Pleated，β-corner，and Randon wind)of samples can be calculated, and we find that all samples contain almost the same structure contents.
Key words：HSA;B6;Fluorescence life time;CD spectra
王建林2，付连春3，周实武4，陈志坚1，吕文波1，叶学敏1，孟广政5，宋增福1* . 维生素B6与人血清白蛋白的相互作用[J]. 光谱学与光谱分析, 2005, 25(06): 912-915.
WANG Jian-lin2, FU Lian-chun3, ZHOU Shi-wu4, CHEN Zhi-jian1, Lü Wen-bo1, YE Xue-min1, MENG Guang-zheng5, SONG Zeng-fu1*. The Interaction of Vitamin B6 with the Human Serum Albumin . SPECTROSCOPY AND SPECTRAL ANALYSIS, 2005, 25(06): 912-915.
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