达旦黄-曲通X-100体系共振光散射法测定蛋白质

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摘要：基于在Triton X-100存在下，蛋白质与达旦黄作用使得体系的共振光散射增强，在λ＝492 nm处光散射强度最大，增强作用的强弱与蛋白质的含量成正比，据此建立了共振光散射测定蛋白质的新方法。此方法对牛血清白蛋白的检出限达到17.7 ng·mL^-1，线性范围为0.03～0.9 μg·mL^-1，用于合成样与人血清样品的分析，取得了令人满意的结果。同时也研究了人血清白蛋白、鸡蛋白蛋白、溶菌酶、胰蛋白酶与达旦黄的作用。

关键词：共振光散射;蛋白质;达旦黄;曲通X-100

Abstract：A resonance light scattering method for the determination of trace proteins was developed. In the presence of Triton X-100, proteins reacted with Titan yellow to form a combination product, resulting a significant enhancement of resonance light scattering(RLS). The ΔI_RLS was directly proportional to the concentration of protein in the range of 0.03-0.9 μg·mL^-1, with the detection limit 17.7 ng·mL^-1 for BSA. This method was applied to the determination of the proteins in synthetic and human serum samples, and compared to the CBB method, with satisfactory results.

Key words：Resonance light scattering;Proteins;Titan yellow;Triton X-100

收稿日期: 2003-12-06 修订日期: 2004-03-18

中图分类号:

O635.3

通讯作者: 冯素玲

引用本文:

冯素玲, 王瑾, 樊静. 达旦黄-曲通X-100体系共振光散射法测定蛋白质[J]. 光谱学与光谱分析, 2005, 25(06): 927-929.
FENG Su-ling, WANG Jin, FAN Jing. Microdetermination of Proteins with Titan Yellow by the Resonance Light Scattering Method . SPECTROSCOPY AND SPECTRAL ANALYSIS, 2005, 25(06): 927-929.

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https://www.gpxygpfx.com/CN/Y2005/V25/I06/927