Study on Modeling Method of Total Viable Count of Fresh Pork Meat Based on Hyperspectral Imaging System
WANG Wei1, PENG Yan-kun1, ZHANG Xiao-li2
1. College of Engineering, China Agricultural University, Beijing 100083, China 2. Department of Biology, Georgia State University, P.O.Box 4010, Atlanta, Georgia, USA
Abstract:Once the total viable count (TVC) of bacteria in fresh pork meat exceeds a certain number, it will become pathogenic bacteria. The present paper is to explore the feasibility of hyperspectral imaging technology combined with relevant modeling method for the prediction of TVC in fresh pork meat. For the certain kind of problem that has remarkable nonlinear characteristic and contains few samples,as well as the problem that has large amount of data used to express the information of spectrum and space dimension, it is crucial to choose a logical modeling method in order to achieve good prediction result. Based on the comparative result of partial least-squares regression (PLSR), artificial neural networks (ANNs) and least square support vector machines (LS-SVM), the authors found that the PLSR method was helpless for nonlinear regression problem, and the ANNs method couldn’t get approving prediction result for few samples problem, however the prediction models based on LS-SVM can give attention to the little training error and the favorable generalization ability as soon as possible, and can make them well synchronously. Therefore LS-SVM was adopted as the modeling method to predict the TVC of pork meat. Then the TVC prediction model was constructed using all the 512 wavelength data acquired by the hyperspectral imaging system. The determination coefficient between the TVC obtained with the standard plate count for bacterial colonies method and the LS-SVM prediction result was 0.987 2 and 0.942 6 for the samples of calibration set and prediction set respectively, also the root mean square error of calibration (RMSEC) and the root mean square error of prediction (RMSEP) was 0.207 1 and 0.217 6 individually,and the result was considerably better than that of MLR, PLSR and ANNs method. This research demonstrates that using the hyperspectral imaging system coupled with the LS-SVM modeling method is a valid means for quick and nondestructive determination of TVC of pork meat.
Key words:Fresh pork meat;Total viable count of bacteria;Hyperspectral imaging system;Least square support vector machines
王 伟1,彭彦昆1,张晓莉2 . 基于高光谱成像的生鲜猪肉细菌总数预测建模方法研究[J]. 光谱学与光谱分析, 2010, 30(02): 411-415.
WANG Wei1, PENG Yan-kun1, ZHANG Xiao-li2 . Study on Modeling Method of Total Viable Count of Fresh Pork Meat Based on Hyperspectral Imaging System . SPECTROSCOPY AND SPECTRAL ANALYSIS, 2010, 30(02): 411-415.
[1] Champiat D, Matas N, Monfort B, et al. Luminescence, 2001, 16: 193. [2] Hoon H S, Welson W W, Jeong S S. Immunology Letters, 2006, 106(2): 191. [3] Durant J A, Young C R, Nisbet D J. International Journal of Food Microbiology, 1997, 38(2): 181. [4] Nathalie Y F, Mulchandani A, Chen W. Analytical Biochemistry, 2001, 289: 281. [5] Sylvie P, Francoise D, Burkhard M. Molecular and Cellular Probes, 2004, 18(6): 409. [6] Ellis D I, Broadhurst D, Goodacre R. Analytica Chimica Acta, 2004, 514: 193. [7] Ellis D I, Broadhurst D, Kell D B. Appl. Environ. Microbiol, 2002, 68: 2822. [8] Duboisa J, Lewisb E N. Calvey E M. Food Microbiology, 2005, 22: 577. [9] Hon H, Peng Y, Lu R. Transactions of the ASAE, 2007, 50(3): 963. [10] Qiao J, Wang N, Ngadi M O, et al. Meat Science, 2007, 76: 1. [11] Kim M S, Chen Y R, Mehl P M. Transactions of the ASAE, 2001, 44(3): 721. [12] Lu R, Peng Y. Laser Focus World, 2005, 41: 99. [13] Peng Y, Lu R. Postharvest Biology and Technology, 2008, 48(1): 52. [14] Park B, Windham W R, Lawrence K C, et al. Proceedings of SPIE, 2002a, 4816: 308. [15] Park B, Lawrence K C, Windham W R, et al. ASAE Paper, 2002b, (2): 313. [16] Lu R, Chen Y R, Park B, et al. ASAE Paper, 1999, (99): 3120. [17] Vapnik V N. The Nature of Statistical Learning Theory. New York: Springer-Verlag, 2005. [18] Vapnik V N. IEEE Trans Neural Network (S1045-9227). 1999, 10(5): 988. [19] Suykens J A K, Vandewalle J. Neural Network Letters (S1057-7122), 1999, 19(3): 293. [20] Gestel T V, Suykens J A K. IEEE Transactions on Neural Network, 2001, 12: 809. [21] Kowk J T. IEEE Transactions on Neural Network, 2000, 11: 1162. [22] Law M H, Kowk J T. Proceedings of the 12th European Conference on Machine Learning. Freiburg, Germanny, 2001. 312.