Abstract：The optical response from a mechanical stimulus signal oscillating system of NADH,DPIP and O2 with the participant of lactate dehyrogenase (LDH) was studied with UV/Vis Spectrometer in the present paper. The signal transduction efficiency of the system was largely improved by the catalysis of LDH,when DPIP.∶NADH was 1∶4.5 (mole ratio)without lactate,and the average period of system was shortened from 108 to 34 min,and to 29 min with lactate existing.It was presumed that the accelerating effect of LDH was mainly brought by the activation of NADH at its catalysis center,and in addition,brought by the supplement of NADH through the dehydrogenation of lactate. The results indicated that the catalysis of enzyme would be put up also when only one of the two substrates existed.
蒋本国,张乐,范圣第. LDH参与的机械-光信号转换体系的吸收光谱研究[J]. 光谱学与光谱分析, 2008, 28(03): 609-611.
JIANG Ben-guo,ZHANG Le,FAN Sheng-di. The Spectrum Studies of Mechano-Optical Signal Transduction with Participation of LDH. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2008, 28(03): 609-611.
