Three-Dimensional Excitation Emission Matrix Fluorescence Spectroscopic Characterization of Loosely Bound and Tightly Bound Extracellular Polymeric Substances of Sludge
OUYANG Er-ming1, 2, WANG Wei1, LONG Neng3, LI Huai4
1. Department of Environmental Science and Engineering, Tsinghua University, Beijing 100084, China 2. School of Civil Engineering and Architecture, Nanchang University, Nanchang 330031, China 3. School of Civil and Environmental Engineering, University of Science and Technology Beijing, Beijing 100083, China 4. Department of Environmental Science and Engineering, Chang’an University, Xi’an 710054，China
Abstract：Three-dimensional excitation emission matrix fluorescence spectroscopy (3DEEM)was applied to characterize the loosely bound extracellular polymeric substances (LB-EPS)and tightly bound extracellular polymeric substances (TB-EPS)extracted from aerobic sludge in wastewater treatment. The results showed that three fluorescence peaks were identified in three-dimensional excitation emission matrix fluorescence spectra of the LB-EPS and TB-EPS: peak B (λex/λem=270-280 nm/345-360 nm), peak C (λex/λem=330-340 nm/410-430 nm)and peak D (λex/λem=390 nm/450-470 nm). The peak B was attributed to the protein-like fluorophores, peak C to the visible fulvic-like fluorophore and peak D to the humic-like fluorophore. Results of the peak intensity showed that the major component of the LB-EPS and TB-EPS is protein-like organic matter, then followed by (from high to low): fulvic-like organic matter and humic-like organic matter. The effects of both pH and concentration were significant to the 3DEEMs of LB-EPS and TB-EPS, but the degree is not the same. LB-EPS has the particular chemical structures which are different from the TB-EPS.
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