1. 广西科学院生物物理实验室，广西 南宁 530003 2. 美国东卡罗莱纳大学物理系，Greenville, NC 27858-4353, USA
FTIR-HATR to Identify β-Thalassemia and Its Mechanism Study
PENG Li-xin1, WANG Gui-wen1*, YAO Hui-lu1, HUANG Shu-shi1, WANG Yi-bing1, TAO Zhan-hua1, LI Yong-qing2
1. Biophysics Laboratory, Guangxi Academy of Sciences, Nanning, Guangxi 530003, China 2. Department of Physics, East Carolina University, Greenville, NC 27858-4353, USA
Abstract：Fourier transform infrared spectroscopy (FTIR)associated with horizontal attenuated total reflectance (HATR)was firstly used to diagnose β-thalassemia patients. With excellent linearity (r=0.997) and reproducibility (RSD＜4%), FTIR-HATR shows an order-of-magnitude increase in IR absorption bands over the single-path transmission FTIR. Based on above, spectra from 37 patients’ and 68 health samples indicated several observable differences in IR vibrational spectra of the Hb lysates between the β-thalassemia major patients and control: (1)Because of decreasing hemoglobin, the peak intensities are obviously lower in β-thalassemia group that is consistent with index from routine hemoglobin diagnosis. (2)In 1 750-1 500 cm-1 region, slight decrease at 1 652 cm-1(α-helix), 1 638 and 1 628 cm-1 bands but mild increase at 1 682 cm-1 all demonstrate structure changes by both Fourier self-deconvolution and second derivative spectra. (3)More importantly, difference spectra substantially demonstrate decreased intensities at 1 440, 1 453, 1 479 cm-1 bands arising from CH2/CH3 deformation vibration of phospholipids but increased intensities at 1 150 cm-1 band originating from C—O stretching vibration of carbohydrate and at 1 081 and 1 053 cm-1 bands that are attributed to 2,3-diphosphoglycerate (DPG)in β-thalassemia major group. Statistical analysis demonstrates significant difference of DPG/phospholipids ratio between two groups. All the samples can be 100% correctly classified into groups on the basis of this ratio. These finding could help understand possible mechanism for diagnosing thalassemia. It makes large-scale screening of thalassemia by FTIR a possibility.
彭立新1,王桂文1*,姚辉璐1,黄庶识1,王一兵1,陶站华1,黎永青2 . FTIR-HATR诊断β-地中海贫血及其机理研究[J]. 光谱学与光谱分析, 2009, 29(05): 1232-1236.
PENG Li-xin1, WANG Gui-wen1*, YAO Hui-lu1, HUANG Shu-shi1, WANG Yi-bing1, TAO Zhan-hua1, LI Yong-qing2 . FTIR-HATR to Identify β-Thalassemia and Its Mechanism Study . SPECTROSCOPY AND SPECTRAL ANALYSIS, 2009, 29(05): 1232-1236.
[1] ZHANG Jun-wu, LONG Gui-fang(张俊武，龙桂芳). The Hemoglobin & Hemoglobinopathies(血红蛋白与血红蛋白病). Nanning: Guangxi Science & Technology Press(南宁:广西科技出版社), 2003. [2] Yong K N, Wadsworth D, Langlois S, et al. Clin. Genet., 1999, 55(1): 20. [3] Xu X M, Zhou Y Q, Luo G X, et al. J. Clin. Pathol., 2004, 57(5): 517. [4] Li D, Liao C, Li J, et al. Eur. J. Obstetrics Gynecology and Reproductive Biology, 2006, 128(1-2): 81. [5] Lau Y L, Chan L C, Chan Y Y, et al. New England and Journal of Medicine, 1997, 336(18): 1298. [6] Fucharoen S, Winichagoon P, Wisedpanichkij R, et al. Clin. Chem., 1998, 44(4): 740. [7] Arrondo J L, Goni F M. Prog. Biophys. Mol. Biol., 1999, 72(4): 367. [8] Barth A, Zscherp C. Q. Rev. Biophys., 2002, 35(4): 369. [9] Gilmanshin R, Williams S, Callender R H, et al. Biochemistry, 1997, 36(48): 15006. [10] Iwaki M, Osyczka A, Moser C C, et al. Biochemistry, 2004, 43(29): 9477. [11] WU Jin-guang(吴瑾光). Techniques and Application of FTIR Spectra in Recent Time(近代傅里叶变换红外光谱技术及应用). Beijing: Scientific and Technical Documents Press(北京: 科学技术文献出版社), 1994. [12] Li Q B, Sun X J, Xu Y Z, et al. Clin. Chem., 2005, 51(2): 346. [13] Argov S, Ramesh J, Salman A, et al. J. Biomed. Opt., 2002, 7(2): 248. [14] LING Xiao-feng, XU Yi-zhuang, WANG Li-xin, et al(凌晓锋, 徐怡庄, 王立新, 等). Chem. J. Chinese Universities(高等学校化学学报), 2007, 28(3): 445. [15] PAN Qing-hua, WANG Bing-bing, LAI Guo-qiao, et al(潘庆华，王冰冰，来国桥, 等). Chem. J. Chinese Universities(高等学校化学学报), 2007, 28(5): 843. [16] Benedetti E, Palatresi M P, Vergamini P, et al. Leukemia Research, 1985, 9(8): 1001. [17] Schultz C P, Liu K Z, Johnston J B, et al. Leukemia Research, 1996, 20(8): 649. [18] Petibois C, Rigalleau V, Melin A M, et al. Clin. Chem., 1999, 45(9): 1530. [19] Petrich W, Dolenko B, Fruh J, et al. Applied Optics, 2000, 39(19): 3372. [20] Liu K Z, Tsang K S, Li C K, et al. Clin. Chem., 2003, 49(7): 1125. [21] Byler D M, Susi H. Biopolymers, 1986, 25(3): 469. [22] Byler D M, Brouillette J N, Susi H. Spectroscopy, 1986, 1(3): 29. [23] Cheung L C, Storm C B, Gabriel B W, et al. Analytical Biochemistry, 1984, 137(2): 481. [24] Wong P T, Wong R K, Caputo T A, et al. Proc. Natl. Acad. Sci. USA, 1991, 88(24): 10988. [25] Cohenford M A, Rigas B. Proc. Natl. Acad. Sci. USA, 1998, 95(26): 15327. [26] Melin A M, Perromat A, Deleris G. Archives of Biochemistry and Biophysics, 2001, 394(2): 265. [27] Lasch P, Pacifico A, Diem M. Biopolymers, 2002, 67(4-5): 335. [28] WANG Jing-yan, ZHU Sheng-geng, XU Chang-fa(王镜岩，朱圣庚，徐长法). Biochemistry(生物化学). Beijing: Higher Education Press(北京:高等教育出版社), 2002. [29] Ting Y L, Naccarato S, Qualtieri A, et al. British Journal of Haematology, 1994, 88(3): 547. [30] Labotka R J, Honig G R. American Journal of Hematology, 1980, 9(1): 55.
