Analysis of the Degrading Products of Carbofuran by Sphingomonas sp. with GC/MS and GC/FTIR
WU Jun1,HONG Qing1, 2,CHEN Yi-nan1,LI Shun-peng1*
1. College of Life Science，Nanjing Agricultural University，Key Lab of Microbiological Engineering Agricultural Environment，Ministry of Agriculture，Nanjing 210095，China 2. Institute of Soil Science，Chinese Academy of Sciences，State Key Laboratory of Soil and Sustainable Agriculture，Nanjing 210008，China
Abstract：In different degrading phases，the degrading products of carbofuran by CDS-1(Sphingomonas sp.) were extracted by acetone. According to the analysis of gas chromatography/mass spectrometry (GC/MS)，carbofuran-7-phenol was identified as the initial degrading product, and an unknown metabolite with molecular weight of 182 was determined as 2-hydroxyl-3-tertiarybutylalcohol-phenol, which was the next degrading product of carbofuran-7-phenol. According to the analysis of GC/MS and gas chromatography/Fourier transform infrared spectrum (GC/FTIR)，the main component of the compounds that appeared in the final phase of degradation which could produce volatile pungent odor，was identified as carvone.
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