Analysis of the Extraction of Red Pellicle of Walnut (Juglans regia L. ) by Ultraviolet-Visible Spectra and HPLC-ESI-MSn
WANG Ke-jian1, 2, HAO Yan-bin2, QI Jian-xun2, HU Xiao-song1*
1. College of Food Science & Nutritional Engineering, China Agricultural University, Beijing 100083, China 2. Institute of Forestry & Pomology, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100093, China
Abstract：The extraction of the red pellicle of walnut (Juglans regia L. ) was analyzed by UV-visible spectra and HPLC-ESI-MSn (high-performance-liquid-chromatography coupled with electrospray ionization mass spectrometry). The extraction in ethanol-HCl showed two absorption peaks at 560 and 591 nm respectively in the UV-Vis spectrum; after purified by lead acetate and thin-layer-chromatography, the extraction in ethanol-HCl showed 4 absorption peaks at 340, 370, 552 and 585 nm respectively. These results testified that the anthocyanin was in the extraction. Six molecular ion peaks (m/z) occurred on MS: 301, 481, 633, 783, 785 and 950, which was identified as ellagic acid, Hexahydroxydiphenoyl(HHDP)-glucose, Galloyl-HHDP-glucose, Di-HHDP-glucose, Di-Galloyl-HHDP-glucose, and HHDP-Valoneoyl-glucose respectively.
Key words：Red Pellicle of walnut;Extraction;Ultraviolet-visible spectra;HPLC-ESI-MSn
王克建1, 2,郝艳宾2,齐建勋2,胡小松1* . 红色核桃仁种皮提取物紫外-可见光谱和质谱分析[J]. 光谱学与光谱分析, 2009, 29(06): 1668-1671.
WANG Ke-jian1, 2, HAO Yan-bin2, QI Jian-xun2, HU Xiao-song1*. Analysis of the Extraction of Red Pellicle of Walnut (Juglans regia L. ) by Ultraviolet-Visible Spectra and HPLC-ESI-MSn . SPECTROSCOPY AND SPECTRAL ANALYSIS, 2009, 29(06): 1668-1671.
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