The Secondary Structure of Winter-Wheat-Bran Antifreeze Protein Determined by FTIR and CD Spectrum
ZHANG Chao1, 2, ZHANG Hui2*, ZHAO Xiao-yan1, MA Yue1, YAO Hui-yuan2
1. National Vegetable Engineering Research Center, Vegetable Research Center, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China 2. Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China
Abstract：The secondary structure of winter wheat bran antifreeze protein was analyzed by FTIR and CD spectra. The characteristic peaks of the secondary structure of the winter wheat bran antifreeze protein were overlapped from the FTIR analysis. The overlapped peaks of amide Ⅰ and Ⅲ of the winter wheat bran antifreeze protein were fitted by the Gauss peak to show the characteristic peaks of the α-helix, β-sheet, β-turn and random coil. The CD spectrum was evaluated by the Chen-Yang principle and analyzed by the least squares method. Furthermore, the difference between the two analyses was discussed. The final results showed that the α-helix, β-sheet, β-turn and random coil of the winter wheat bran antifreeze protein was 15.05%-15.87%, 36.00%-36.92%, 17.31%-17.75%, and 30.30%-30.82%, respectively. The result would be helpful to the study of the antifreeze mechanism of the winter wheat bran antifreeze protein.
张超1, 2,张晖2*,赵晓燕1,马越1,姚惠源2 . 使用圆二色性光谱和红外光谱研究冬小麦麸皮抗冻蛋白的二级结构[J]. 光谱学与光谱分析, 2009, 29(07): 1764-1767.
ZHANG Chao1, 2, ZHANG Hui2*, ZHAO Xiao-yan1, MA Yue1, YAO Hui-yuan2 . The Secondary Structure of Winter-Wheat-Bran Antifreeze Protein Determined by FTIR and CD Spectrum. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2009, 29(07): 1764-1767.
[1] Kristiansen E, Zachariassen K E. Cryobiology, 2005, 51: 262. [2] Zhang C, Zhang H, Wang L, et al. Journal of Agricultural Food Chemistry, 2007, 55: 7654. [3] WANG Ao-jin, HU Kun-sheng, SUN Yong-tai(王敖金，胡坤生，孙永泰). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 1994, 14(3): 41. [4] HONG Fa-shui(洪法水). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2003, 23(3): 583. [5] XIE Meng-xia, LIU Jun(谢孟峡, 刘俊). Chemical Journal of Chinese Universities(高等学校化学学报), 2003, 24: 226. [6] Byler D M, Brouillette J N, Susi H. Spectroscopy, 1986, 1: 29. [7] XIAO He-lan, SUN Su-qin, ZHOU Qun, et al(肖和兰, 孙素琴, 周群, 等). Chinese Journal of Atomic and Molecular Physics(原子与分子物理学报), 2003, 20: 211. [8] WU Jin-guang(吴瑾光). Application and Technology of Modern FTIR(近代傅里叶变换红外光谱技术及应用). Beijing：Scientific and Technical Documents Publishing House(北京：科学技术文献出版社), 1994. 23. [9] Carter D C, Ho J X. Adv. Protein Chemistry, 1994, 45: 153. [10] Watanabe R, Masui R, Mikawa T, et al. J. Biochem. (Tokyo), 1994, 116: 969. [11] LIU Yuan, XIE Meng-xia, KANG Juan(刘媛, 谢孟峡, 康娟). Acta Chim. Sinica(化学学报), 2003, 61: 1305. [12] Surewicz W K, Mantsch H H. Biochimica et Biophysica Acta, 1988, 952: 115. [13] Mallapragada S K, Peppas N A. Journal of Applied Polymer Science Part B-Polymer Physics, 1996, 34: 1339. [14] Somania P R, Marimuthua R, Viswanatha A K, et al. Polymer Degradation and Stability, 2003, 79: 77. [15] YAN Long-fei, SUN Zhi-rong(阎隆飞, 孙之荣). Structure of Protein(蛋白质分子结构). Beijing: Tsinghua University Press(北京：清华大学出版社), 1999. 120. [16] YANG Hai-ling, GAO Bo, ZENG Qing-yin, et al(杨海灵，高波，曾庆银，等). Chemical Journal of Chinese Universities(高等学校化学学报), 2003, 24: 1472. [17] LU Yan, ZHANG Wei-wei, WANG Gong-ke(卢雁, 张玮玮, 王公轲). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2008, 28(1): 88. [18] HE Jin-tian, WANG Gai-zhen, SONG Hou-yan(贺进田, 王改珍, 宋后燕). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2006, 26(7): 1209. [19] Jagdeesh B. Biochim. Biophys. Acta, 1992, 1120: 123.
