NIRS Method for Determination of Meat and Bone Meal Content in Ruminant Concentrates
YANG Zeng-ling1,2,HAN Lu-jia1,2*,LI Qiong-fei2,3,LIU Xian1,2
1. College of Engineering, China Agricultural University, Beijing 100083, China 2. Key Laboratory of Modern Precision Agriculture System Integration, Ministry of Education, Beijing 100083, China 3. Shanghai Finance University, Shanghai 201209, China
Abstract：Feed contaminated with MBM is commonly accepted as the main transmission carrier of bovine spongiform encephalopathy (BSE). To prevent BSE many countries have banned MBM as a feed ingredient. In the People’s Republic of China, the ban was first applied to ruminant feed. In order to investigate the feasibility of near infrared diffuse reflectance spectroscopy method for rapidly quantitative determination of meat and bone meal content in ruminant concentrates, 225 representatively commercial ruminant concentrates samples and 75 meat and bone meal (including cattle, sheep, pig and poultry meat and bone meal) samples were collected in the People’s Republic of China. Two hundred twenty five ruminant concentrates samples of adulterated meat and bone meal (0.5%-35%) were prepared including 135 calibration samples and 90 independent validation samples. For the calibration set samples, 3 samples were prepared at each concentration. For validation set samples, 2 samples were prepared at each concentration. Any one commercial ruminant concentrates was used once only. The spectra were scanned by raster near infrared diffuse reflectance spectroscopy instrument, and the effect of spectrum pretreatment methods (mathematic pretreatments and scatter correction) and spectrum region (visible and NIR) on the calibration results was considered. The calibration equation was established by modified partial least squares method. The result showed that the calibration gave r2 of 0.979, a standard error of calibration (SEC) of 1.522% and a standard error of cross validation (SECV) of 1.582%. The 90 independent validation samples were used to validate the quantitative equation. The r2, a standard error of prediction (SEP) and ratio of performance to standard deviation (RPD) were 0.972, 1.764% and 5.99 respectively. The results of this study indicated that near infrared diffuse reflectance spectroscopy method could provide rapidly quantitative prediction for meat and bone meal percent in ruminant concentrates. This method was significant in practice for enriching the rapidly quantitative methods of determining animal feed materials.
Key words：Near infrared diffuse reflectance spectroscopy;Quantification;Ruminant;Feed;Meat and bone meal
杨增玲1,2,韩鲁佳1,2*,李琼飞2,3,刘贤1,2. 反刍动物精料补充料中肉骨粉快速检测近红外光谱法[J]. 光谱学与光谱分析, 2008, 28(06): 1278-1282.
YANG Zeng-ling1,2,HAN Lu-jia1,2*,LI Qiong-fei2,3,LIU Xian1,2. NIRS Method for Determination of Meat and Bone Meal Content in Ruminant Concentrates. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2008, 28(06): 1278-1282.
[1] European Communities, Bovine Spongiform Encephalopathy(BSE), 3<sup>rd</sup> ed. Vademecum, Brussels 16 October 1998. [2] Gizzi G, van Raamsdonck LWD, Baeten V, et al. Sci. Tech. Rev., 2003, 22(1): 311. [3] Ansfield M, Reaney S D, Jackman R. Food Agri Immun., 2000, 12: 285. [4] Gibson U E M, Heid C A, Williams P M. Genome Res., 1996, 6: 995. [5] Hofmann K, Muller E, Fischer K. Fleischwirtschaft, 1999, 79(10);92. [6] LI Jun-xia, MIN Shun-geng, ZHANG Hong-liang, et al（李君霞，闵顺耕，张洪亮, 等）. Spectroscopy and Spectral Analysis（光谱学与光谱分析），2006，26（5）：833. [7] LIU Yan-de, YING Yi-bin（刘燕德，应义斌）. Spectroscopy and Spectral Analysis（光谱学与光谱分析），2006，26（8）：1454. [8] WANG Jia-jun, WANG Fan, MA Ling（王家俊，汪 帆，马 玲）. Spectroscopy and Spectral Analysis（光谱学与光谱分析），2006，26（10）：1858. [9] Murray I, Aucott L S, Pike I H. Journal of Near Infrared Spectroscopy, 2001, 9: 297. [10] Piraux F, Dardenne P. In: Davies A M C., Giangiacomo R. (Eds.), Proceedings of the Ninth International Conference on Near Infrared Spectroscopy. West Sussex: NIR Publications, Chichester, 2000. 535. [11] Fernandez M, Martinez A, Modrono S, et al. In: Davies A M C., Cho, R.K. (Eds.), Proceedings of the 10th International Conference on Near Infrared Spectroscopy. Chichester: NIR Publications, 2002. 307. [12] Garrido A, Marin M D, Guerrero J E, et al. Proceedings of the 10th International Conference on Near Infrared Spectroscopy. West Sussex: NIR Publications, Chichester, 2002. 145. [13] Dolores C, Ana Garrido-Varo, Guerrero J E, et al. Animal Feed Science and Technology, 2004, 116: 333. [14] NIU Zhi-you, HAN Lu-jia, SU Xiao-ou, et al（牛智有，韩鲁佳，苏晓鸥, 等）. Transactions of the Chinese Society of Argricultural Engineering(CSAE)(农业工程学报），2005，21（4）：155. [15] Garrido-Varo A, Pérez-Marín D, Guerrero J E, et al. Biotechnol. Agron. Soc. Environ., 2005, 9(1): 3. [16] CHU Xiao-li, YUAN Hong-fu, WANG Yan-bin, et al（褚小立，袁洪福，王艳斌, 等）. Spectroscopy and Spectral Analysis（光谱学与光谱分析），2004，24（6）：666.
