Study of the Interaction between Methotrexate and Bovine Serum Albumin by Spectrometry
XIE Wen-jun1, FENG Yu-ping1*, CAO Sheng-li2, ZHAO Yu-fen1
1. Key Laboratory of Bioorganic Phosphorus Chemistry and Chemical Biology(Ministry of Education), Department of Chemistry, Tsinghua University, Beijing 100084, China 2. Department of Chemistry, Capital Normal University, Beijing 100037, China
Abstract：The interaction of methotrexate (MTX) and bovine serum albumin (BSA) was studied by fluorescence and absorption spectra. The results show that methotrexate strongly quenched the fluorescence of bovine serum albumin, and the quenching mechanism is a static quenching procedure. The binding constants and the number of binding sites between MTX and BSA at different temperatures were calculated. Furthermore, the enthalpy and entropy changes in the interaction were also obtained, and the primary binding pattern between MTX and BSA was interpreted as hydrophobic interaction.
Key words：Methotrexate (MTX);Bovine serum albumin (BSA);Fluorescence spectra;Absorption spectra;Interaction
谢文俊1,冯玉萍1*,曹胜利2,赵玉芬1 . 光谱法研究甲氨喋呤与牛血清白蛋白的相互作用[J]. 光谱学与光谱分析, 2006, 26(10): 1876-1879.
XIE Wen-jun1, FENG Yu-ping1*, CAO Sheng-li2, ZHAO Yu-fen1 . Study of the Interaction between Methotrexate and Bovine Serum Albumin by Spectrometry . SPECTROSCOPY AND SPECTRAL ANALYSIS, 2006, 26(10): 1876-1879.
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