Studies on the Mechanism of the Interaction between Hydrazide-Podophyllic Ni(Ⅱ), Co(Ⅱ), Zn(Ⅱ) Metal Complexes and DNA
WANG Ping-hong1, ZHANG Qi1*, YUAN Wen-bing1, WANG Liu-fang2, SONG Yu-min3, LIU Hui-hong1
1. Province Key Laboratory of Refined Chemistry, Hainan University, Haikou 570228, China 2. State Key Laboratory of Applied Organic Chemistry, Lanzhou University, Lanzhou 730000, China 3. College of Chemistry and Chemical Engineering, Northwest Normal University, Lanzhou 730070, China
Abstract：The mechanism of the interaction between hydrazide-podophyllic (HDPP) metal (Me) complexes and calf thymus (ct) DNA in Tris buffer (pH 7.08) was studied by viscosity measurements, electronic absorption, gel electrophoresis, and ethidium bromide (EB) fluorescence spectroscopy. The results from varied experiments show that the intensity of the maximal absorption peaks from absorption spectra is weakened in the presence of DNA compared with that in the absence of DNA. Meanwhile, DNA can remarkably quench the emission intensity of the complex Me-HDPP system. The Me-HDPP complexes can increase the viscosity of ct DNA slightly and catalyze the cleavage of super coiled pBR322 DNA to the nicked form. The complexes of Ni-HDPP and Co-HDPP can be bound to ct DNA mainly by interaction, while the partial interaction of Zn-HDPP and ct DNA is the major modes. The binding constant of Me-HDPP complexes with ct DNA was determined.
Key words：HDPP;Metal complexes;Ethidium bromide;Calf thymus DNA
王平红1,张岐1*,袁文兵1,王流芳2,宋玉民3,刘慧宏1 . 鬼臼酰肼金属Ni(Ⅱ), Co(Ⅱ), Zn(Ⅱ)配合物与DNA分子作用机制的研究[J]. 光谱学与光谱分析, 2006, 26(07): 1298-1302.
WANG Ping-hong1, ZHANG Qi1*, YUAN Wen-bing1, WANG Liu-fang2, SONG Yu-min3, LIU Hui-hong1 . Studies on the Mechanism of the Interaction between Hydrazide-Podophyllic Ni(Ⅱ), Co(Ⅱ), Zn(Ⅱ) Metal Complexes and DNA. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2006, 26(07): 1298-1302.
[1] ZHANG Li-he, ZHUO Ji-cang, MA Ling-tai, et al(张礼和, 卓济苍，马灵台，等). Study on the Medicines as the Target of Nucleic Acid(以核酸为作用靶的药物研究). Beijing: Science Press(北京: 科学出版社), 1997. 1. [2] YE Yong, HU Ji-ming, ZENG Yun-e(叶 勇, 胡继明, 曾云鹗). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2001, 21(5): 623. [3] CHEN Hui-li, YANG Pin (陈绘丽, 杨 频). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2002, 22(6): 983. [4] QU Jian-qiang(曲建强). Thesis for the Doctorate of Lanzhou University (兰州大学博士论文), 2002. [5] Reichmann M E, Rice S A, Thomas C A, et al. J. Am. Chem. Soc., 1954, 76: 3047. [6] Uma M P, Palaniandavar M. Journal of Inorganic Biochemistry, 2004, 98: 219. [7] YE Yong, HU Ji-ming, ZENG Yun-e(叶 勇, 胡继明, 曾云鹗). Chinese Journal of Inorganic Chemistry(无机化学学报) , 1998, 14(1): 84. [8] LIN Qiu-yue, HU Rui-ding, ZHENG Xiao-hua(林秋月，胡瑞定，郑孝华). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2004, 24(8): 988. [9] Mary H G, Kun C W, Willian R B. Biochemistry, 1976, 15: 4339. [10] LI Zhi-liang, BI Qiong-si, LI Jun-zhong, et al(李志良, 毕琼斯, 李俊忠, 等) . Chinese Chemistry Bulletin(化学通报)，1997, (6): 52. [11] LI Zhi-liang, CHEN Jian-hua, ZHANG Kai-cheng, et al(李志良, 陈建华, 章开诚, 等). Science in China (Series B)(中国科学, B辑), 1991, 21: 1193. [12] JIANG Chong-qiu, HE Ji-xiang, WANG Jin-shan (江崇球，贺吉香，王金山) . Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2002, 22(1): 103. [13] ZHANG Li-jin, MIN Shun-geng, LI Guo-xue, et al(张立金，闵顺耕，李国学，等). Spectroscopy and Spectral Analysis(光谱学与光谱分析) , 2005, 25(5): 739. [14] Daryono H T, Shunsuke M, Takehiro A, et al. J. Inorg. Biochem., 2001, 85: 219. [15] Palanisamy U M, Mallayan P. Inorganica Chimica Acta, 2004, 357: 901. [16] Kelly J M, Tossi A B, McConnell D J, et al. Nucl. Acids Res., 1985, 13: 6017. [17] Jenkins Y, Friedman A E, Turro N J, et al. Biochemistry, 1992, 31: 10809. [18] Friedman A E, Chambron J C, Sauvage J P, et al. J. Am. Chem. Soc., 1990, 112: 4960. [19] Vaidyanathan V G, Nair B U. J. Inorg. Biochem., 2002, 91: 405. [20] Ichikawa K, Tarnai M, Uddin M K, et al J. Inorg. Biochem., 2002, 91: 437.
