Quantitative Analysis of Phenanthrene and Humic Acid in Solution Based on Fluorescence Method
JIN Dan1, LI Guo-gang2, ZHANG Yu-jun1*, XIAO Xue1, ZHAO Nan-jing1, YIN Gao-fang1, LIU Wen-qing1
1. Key Laboratory of Environmental Optics & Technology, Anhui Institute of Optics and Fine Mechanics, the Chinese Academy of Sciences, Hefei 230031, China 2. China National Environmental Monitoring Center, Beijing 100029, China
Abstract：Polycyclic aromatic hydrocarbons as a universal concern in the monitoring of priority pollutants have low content in the water environment and are interfered with the coexistence of humic acid. The spectra of humic acid and polycyclic aromatic hydrocarbons overlap seriously, so it was difficult to use the conventional methods for rapid quantitative detection. As a model compound of polycyclic aromatic hydrocarbons, Phenanthrene (PHE) was chosen in the experiment. The fluorescence spectra of PHE and humic acid (HA) were investigated by three dimensional fluorescence excitation-emission matrix. The method combined excitation-emission matrix fluorescence spectra with parallel factor analysis and was applied to determine PHE and HA directly. The excitation wavelength changed from 240 to 360 nm at an interval of 5 nm. The emission wavelength varied from 260 to 575 nm at an interval of 5nm. The satisfactory results show that this experiment can be easily performed without paying out time-consuming and complicated procedures.
Key words：Three-dimensional fluorescence spectra;Overlapping of fluorescence spectra;PARAFAC;Phenanthrene;Humic acid
金 丹1,李国刚2,张玉钧1*,肖 雪1,赵南京1,殷高方1,刘文清1 . 菲及腐殖酸混合液荧光法定量分析研究[J]. 光谱学与光谱分析, 2009, 29(08): 2213-2216.
JIN Dan1, LI Guo-gang2, ZHANG Yu-jun1*, XIAO Xue1, ZHAO Nan-jing1, YIN Gao-fang1, LIU Wen-qing1 . Quantitative Analysis of Phenanthrene and Humic Acid in Solution Based on Fluorescence Method . SPECTROSCOPY AND SPECTRAL ANALYSIS, 2009, 29(08): 2213-2216.
[1] Beltran J L, Guiteras J, Ferrer R. J. Chromatogr. A, 1998, 802: 263. [2] Roch Th. Anal. Chim. Acta, 1997, 356: 61. [3] Warner I M, Christian G D, Davidson E R, et al. Anal. Chem., 1977, 49: 564. [4] LI Hong-bin, LIU Wen-qing, WANG Zhi-gang, et al(李宏斌, 刘文清, 王志刚, 等). Chinese Journal of Quantum Electronics(量子电子学报) , 2007, 24(3): 306. [5] ZHAO Nan-jing, LIU Wen-qing, CUI Zhi-cheng, et al(赵南京, 刘文清, 崔志成, 等). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2006, 26(5): 922. [6] Tatiana A D, Victor V F, Irina V G, et al. Appl. Opt., 2002, 41 (24), 5155. [7] Stedmon C A, Markager S, Bro R., Marine Chem., 2003, 82: 239. [8] Tong H Y, Karasek F W. Anal. Chem., 1984, 56: 2129. [9] Beltran J L, Guiteras J, Ferrer R. Anal. Chem., 1998, 70: 1949. [10] Cecil T L, Rutan S C. J. Chromatogr., 1991, 556: 495. [11] Martinez E, Gros M, Lacorte S, et al. J. Chromatogr. A, 2004, 1047(2): 181. [12] Baker A. Environmental Science & Technology, 2001, 35 (5): 948. [13] LI Li，YU Zhi-qiang，SHENG Guo-ying，et al(李 丽, 于志强, 盛国英, 等). Environmental Chemistry(环境化学), 2004, 23(4): 381. [14] XIA Xing-hui, ZHANG Xi, YANG Zhi-feng, et al(夏星辉, 张 曦, 杨志峰, 等). Environmental Science(环境科学), 2006, 27(1): 115.
