Abstract:In the present paper, low-energy N+ ions produced by low-energy ionic radiometer were used to simulate low-energy ions in astrospace implanting HeLa cell. Then, the effect and mechanism of low-energy ion on the cell were studied with FTIR spectroscopic analysis. In this study, low-energy ions were produced and accelerated in vacuum, and cells would be affected by vacuum when they were implanted by low-energy ions, so mineral oil was used to protect cells from water evaporation. Cells were collected after being implanted, then the change in the content and constructional form in cellular macromolecule with infrared spectrometry. Result indicated that the spectroscopic peak position of differently worked cells was obviously different as compared to the control cell (3 300 cm-1). Spectroscopic peak position of all samples except implanting 5×1014 N+·cm-2 removed to longer wavelength, and the peak position in vacuum of 2×1015 N+·cm-2 sample moved to 3 420 cm-1. In addition, the 1 378 and 2 360 cm-1 spectroscopic peak positions in control cell all moved to longer wavelength in every worked group. In a word, FTIR spectroscopic analysis indicates that low-energy ion implantation could arouse change in nucleic acid or protein in HeLa cell.
[1] FENG Hui-yun,YU Li-xiang,LIU Xing-hai,et al. Proceedings of the Third National Conference of Ion Beam Bioengineering and the First International Symposium on Ion Beams. Urumqi, China, 2002. 17. [2] ZHANG Feng-qiu, YA Hui-yuan, SU Ming-jie,et al(张凤秋, 押辉远, 苏明杰, 等). Acta Laser Biology Sinica(激光生物学报),2006, 6(15): 594. [3] ZHANG Feng-Qiu, YA Hui-Yuan, LI Jing-Yuan,et al. Nuclear Science and Techniques, 2006, 4(17): 222. [4] ZHAO Yuan-li, ZHANG Feng-qiu, GE Xiang-hong,et al(赵元黎, 张凤秋, 葛向红, 等). Laser Journal(激光杂志),2007,2(28): 84. [5] LIANG Er-jun, LIU Yi-zhen, LIU She-wen,et al(梁二军, 刘一真, 刘社文, 等). Chinese Journal of Light Scattering(光散射学报),1999, 11(3): 260. [6] SHAO Chun-lin, BI Qiang, YU Zeng-liang(邵春林,毕 强,余增亮). Nuclear Techniques(核技术),1994, 17(1): 25. [7] YU Zeng-liang(余增亮). Introduction of Ion Beam Biotechnology(离子束生物技术引论). Hefei: Anhui Science and Technology Publishing House(合肥:安徽科学技术出版社), 1998. 183. [8] YUAN Shi-bin, WEI Zeng-quan, LI Wen-jian, et al(袁世斌, 卫增泉, 李文建, 等). High Energy Physics and Nuclear Physics(高能物理与核物理), 2003, 27(5): 460. [9] YUAN Shi-bin,WEI Zeng-quan, GAO Qing-xiang(袁世斌,卫增泉,高清祥). Chinese Science Bulletin(科学通报), 2001,46(23): 1966.