Study on the Effect of Electric Field on the Secondary Structure of Lipase by Circular Dichroism
YAO Zhan-quan1, 2, AO Dun-ge-ri-le1, XU Qiang1, YANG Ti-qiang1
1. College of Science and Technology, Inner Mongolia University, Huhhot 010021, China 2. College of Water Conservancy and Civil Architecture Engineering, Inner Mongolia Agricultural University, Huhhot 010018, China
Abstract：After the lipase was treated with electric field for five minutes, the effect of electric field on the secondary structure of lipase was studied by circular dichroism (CD). The results showed that different electric field strength in the range from 0.5 to 6.0 kV·cm-1 has a different effect on the relative contents of α-helix, β-sheet, β-turn and random coil of the lipase, and whose changes were non-monotonous with the raising of electric field. In general, the electric treatment tends to transform the α-helix and β-sheet into β-turn and random coil. The decrease in α-helix and β-sheet ranged respectively from 4.6% to 48.0% and from 13.2% to 35.1%, and the increase in β-turn and random coil ranged respectively from 2.8% to 33.3% and from 0.9% to 48.1%. The result of this study has important meaning to explain the biological effect of electric treatment seeds.
姚占全1, 2, 敖敦格日勒1, 许强1, 杨体强1 . 应用圆二色光谱研究电场对脂肪酶二级结构的影响[J]. 光谱学与光谱分析, 2006, 26(12): 2311-2314.
YAO Zhan-quan1, 2, AO Dun-ge-ri-le1, XU Qiang1, YANG Ti-qiang1 . Study on the Effect of Electric Field on the Secondary Structure of Lipase by Circular Dichroism. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2006, 26(12): 2311-2314.
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