Study on Fenton-Methylene Blue Spectral Analysis Method for Determining the Inhibitory Effect of Quercetin Complexes on Hydroxyl Free Radical
LI Fang1, ZHENG Huai-li2
1. College of Chemistry, Sichuan University, Chengdu 610064, China 2. College of Chemistry and Chemical Engineering, Chongqing University, Chongqing 400044, China
Abstract：In the present, Fenton-Methylene Blue spectral measurement was established, and the new analytical method was applied successfully to determine the inhibitory effect of quercetin (Que) and its complexes on hydroxyl free radical. Methylene Blue(MB) can capture the ·OH produced by Fenton reaction, turning its color from blue to achromatic. Therefore, MB was selected as the indicator of the spectral measure study. The testing conditions optimized were pH 8.0, H2O2 solution (0.3%) 0.50 mL, FeSO4 solution (5 mmol·L-1) 0.50 mL and MB solution(2.56×10-5 mol·L-1)1.0 mL. The experiment results showed that Que-Zn(Ⅱ), Que-Cu(Ⅱ) and Que-Fe(Ⅲ) complexes held significant effects of scavenging action on ·OH radical and were more effective in scavenging ·OH than quercetin itself, showing the good cooperative action between quercetin and trace metal elements. The structure-bioactivity relationship of the coordination complexes was also discussed through analyzing the antioxygenic activity of quercetin-metal complexes.
李方1,郑怀礼2. 黄酮配合物抗自由基活性的亚甲基蓝光谱测定体系的研究[J]. 光谱学与光谱分析, 2006, 26(12): 2294-2297.
LI Fang1, ZHENG Huai-li2. Study on Fenton-Methylene Blue Spectral Analysis Method for Determining the Inhibitory Effect of Quercetin Complexes on Hydroxyl Free Radical. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2006, 26(12): 2294-2297.
[1] HUI Rui-hua, HOU Dong-yan, GUAN Chong-xin, et al(回瑞华，侯冬岩，关崇新，等). Spectroscopy and Spectral Analysis(光谱学与光谱分析)，2005, 25(2): 266. [2] Craig W J. American Journal of Clinical Nutrition, 1999, 70 (3):491. [3] LIN Jin-ming, QU Feng, SHAN Xiao-quan(林金明，屈 锋，单孝全). Chinese Journal of Analytical Chemistry(分析化学)，2002, 30(12): 1507. [4] CHENG Guan-hua, TIAN Yi-ling, YANG Geng-liang, et al(陈冠华，田益玲，杨更亮，等). Spectroscopy and Spectral Analysis(光谱学与光谱分析)，2002, 22(4): 634. [5] Tawa R, Sakurai H. Anal Lett., 1997, 30 (15): 2811. [6] ZENG Xiao-ling(曾小玲). China Journal of Modern Medicine(中国现代医学杂志)，1999, 9(11): 22. [7] LI Gui-rong, YANG Sheng-yuan(李贵荣，杨胜圆). Chemical World(化学世界)，2001, (8): 421. [8] LUO Zong-ming, FANG Yan-xiong, ZHANG Kun, et al(罗宗铭，方岩雄，张焜，等). China Oils and Fats(中国油脂)，2003, 28(3): 58. [9] ZHENG Rong-liang(郑荣梁). Redical Biochemistry(自由基生物化学). Beijing: Higher Education Press(北京：高等教育出版社)，1992.
