Abstract：Four hundred ninety seven rapeseed samples, which feature multi-year, multi-loci and highly variant characteristics, were collected as a raw set. The NIR spectra were pretreated by scatter correction and mathematics treatments, and calibration models of fat acid composition of intact rapeseed were developed by using the algorithm method of modified partial least square(MPLS). Meanwhile, three types of sample cups with different capacity were used to screen the suitable calibration model for rapeseed quality breeding. The results showed that the calibration model of 8-gram-sample was the best, and the calibration determination coefficient was in the range of 0.74-0.98. The calibration effects of 3-gram-sample, which were similar to those of 0.6-gram-sample, were good with high determination coefficient (RSQ1, 1-VR) and low error (SEC, SECV). Therefore, the calibration set with multi-year and multi-loci samples can improve the accuracy and repeatability of NIRS models. The fat acids NIRS models of intact rapeseed developed could be introduced into breeding lines’ selection, mutant screening and germplasm evaluation.
Key words：Near infrared reflectance spectroscopy (NIRS);Rapeseed;Fat acid;Calibration model
吴建国，石春海*，张海珍. 构建整粒油菜籽脂肪酸成分近红外反射光谱分析模型的研究[J]. 光谱学与光谱分析, 2006, 26(02): 259-262.
WU Jian-guo, SHI Chun-hai*, ZHANG Hai-zhen. Study on Developing Calibration Models of Fat Acid Composition in Intact Rapeseed by Near Infrared Reflectance Spectroscopy . SPECTROSCOPY AND SPECTRAL ANALYSIS, 2006, 26(02): 259-262.
[1] LI Qin-chen, WANG Wang-zheng, ZHANG Yu-liang, et al.(李庆春，王文真，张玉良, 等). Acta Agronomica Sinica(作物学报)，1992，18(3)：235. [2] JIN Tong-ming, CUI Hong-chang(金同铭，崔洪昌). Acta Agriculturae Boreali-Sinica(华北农学报)，1994，9(4): 104. [3] Batten G D. Australian Journal of Eperimental Ariculture, 1998, 38: 697. [4] CHU Xiao-li, YUAN Hong-fu, WANG Yan-bin, LU Wan-zhen(褚小立，袁洪福，王艳斌, 陆婉珍). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2004, 24(6): 666. [5] Rernhardt T D, Rbbelen G. In: GCIRC (Ed.), Proc 8th Int Rapeseed Congr, Saskatoon, Canada, 1991. 1380. [6] Daun J K, Clear K M, Williams P. Journal of American Oil Chemists Society, 1994, 71: 1063. [7] Velasco L, Fernandez-Martinez J M, Haro A D. Crop Science, 1996, 36: 1068. [8] Velasco L, Becker H C. Euphytica, 1998, 101: 221. [9] GU Wei-zhu, WANG Yan-xiang(顾伟珠, 汪延祥). Journal of the Chinese Cereals and Oils Association(中国粮油学报)，1995，10(2)：57. [10] ZHANG Ye-hui, ZHAO Long-lian, LI Xiao-wei, et al.(张晔晖，赵龙莲，李晓薇, 等). Acta Laser Biology Sinica(激光生物学报), 1998，7(2): 138. [11] CHENG Wen-jie, TAN Xiao-li, WANG Zhu-yun, et al(陈文杰，谭小力，王竹云, 等). Shanxi Agriculture Science(陕西农业科学), 2002, (8): 6. [12] WU Jian-guo, SHI Chun-hai, ZHANG Hai-zheng, et al(吴建国，石春海，张海珍, 等). Acta Agronomica Sinica(作物学报)，2002，28(3): 421. [13] WU Jian-guo, SHI Chun-hai, FAN Long-jiang(吴建国，石春海，樊龙江). Journal of the Chinese Cereals and Oils Association(中国粮油学报)，2002，17(2): 59. [14] LI Yan-li, SUN Chao-cai, QIAN Xiao-fang, et al(李延莉, 孙超才, 钱小芳, 等). Acta Agriculturae Shanghai(上海农业学报), 2003，19(1): 11. [15] SUN Chuan-jing(孙传经). Gas Chromatography Mechanism and Technique(气相色谱分析原理与技术). Beijing: Chemical Industry Press(北京: 化学工业出版社), 1979. [16] ZHOU Rong-ming, LIU Hou-li(周永明，刘后利). Acta Agronomica Sinica(作物学报)，1987，13：1. [17] Shenk J S, Westerhaus M O. Infrasoft International, Port Matilda, PA, USA, 1993. [18] XU Guang-tong, YUAN Hong-fu, LU Wan-zhen(徐广通，袁洪福，陆婉珍). Spectroscopy and Spectral Analysis (光谱学与光谱分析), 2001, 21(4): 459.
