1. Department of General Surgery, Peking University Third Hospital, Beijing 100191, China 2. College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China
Abstract：To explore the feasibility of quick intraoperative in situ and noninvasive diagnosis of cholangiocarcinoma by Fourier transform infrared (FTIR) spectroscopy, 26 samples of freshly resected cholangiocarcinoma tissues and 43 samples of benign bile duct tissue were collected during surgery. And all fresh samples were measured by FTIR spectroscopy via probe of attenuated total reflection (ATR) without pretreatment immediately after resection. For each spectrum, 12 bands were identified and assigned between 3 800 and 1 000 cm-1. The peak position was found, and the intensity and area of the bands were measured; The ratios of the intensity and area were calculated. Standard statistic analysis was performed. The results illustrated that the FTIR spectra of malignant tissue were significantly different from those of benign tissue: 1. The bands related to lipid: The peak of 2 925 cm-1 shifted to lower wave number significantly (P=0.033); 2. The bands related to nucleic acid and lipids: the ratios of I1 083/I1 460(P=0.005), S1 083/S1 460(P=0.001) and S1 240/S1 460(P=0.025) raised significantly, indicating that the relative quantity of nucleic acid to lipids had evidently increased in malignancy. 3. The bands related to protein: The ratios of I1 550/I1 083(P=0.000)and S1 550/S1 083(P=0.000)reduced significantly, implying that the relative quantity of protein to nucleic acid in malignancy decreased significantly and the secondary structure of protein changed probably. Our primary result illustrated that the FTIR spectroscopy technique maybe a promising method for in situ and quick intraoperative diagnosis of cholangiocarcinoma and has great value for clinical application.
[1] Lazaridis K N, Gores G J. Gastroenterology, 2005, 128: 1655. [2] Olnes M J, Erlich R. Oncology, 2004, 66: 167. [3] Cha J M, Kim M H, Lee S K, et al. Clin. Oncol.(R Coll Radiol), 2006, 18: 669. [4] Tsunoda T, Eto T, Koga M, et al. Jpn. J. Surg., 1989, 19: 691. [5] Yamaguchi K. Aust. N Z J Surg., 1992, 62: 525. [6] Klatskin G. Am. J. Med., 1965, 38: 241. [7] Boerma E J. Surgery, 1990, 108: 572. [8] Bismuth H, Nakache R, Diamond T. Ann. Surg., 1992, 215: 31. [9] Chung Y E, Kim M J, Park Y N, et al. Eur. Radiol., 2008, 18: 2182. [10] SHEN Xue-chu(沈学础). The Techniques and Application of Modern Fourier Transform Infrared Spectroscopy(近代傅里叶变换红外光谱技术及应用). Edited by WU Jin-guang(吴瑾光主编). Beijing: Literature Press of Science and Technology(北京: 科学技术文献出版社), 1994. [11] Lyng F M, Faolain E O, Conroy J, et al. Experimental and Molecular Pathology, 2007, 82: 121. [12] Kretlow A, Wang Q, Kneipp J, et al. Biochim. Biophys. Acta, 2006, 1758(7): 948. [13] LING Xiao-feng, XU Yi-zhuang, WANG Li-xin, et al(凌晓锋, 徐怡庄, 王立新, 等). Chemical Journal of Chinese Universities(高等学校化学学报), 2007, 28(3): 445. [14] Wong P T T, Papavassiliou E D, Rigas B. Appl. Spectrosc., 1991, 45(9): 1563. [15] LI Wei-xiu, ZHENG Quan-qing, WANG Ping, et al(李炜修, 郑全庆, 王 平, 等). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2006, 26(10): 1833. [16] Benedetti E, Palatresi M P, Vergamini P, et al. Appl. Spectrosc., 1986, 40(1): 39. [17] Surewicz W K, Mantsch H H. Biochim. Acta, 1988, 952: 115. [18] Malins D C, Gunselman S J. Proc. Natl. Acad. Sci. USA, 1994, 91: 13038. [19] Cohenford M A, Godwin T A, Cahn F, et al. Gynecol. Oncol., 1997, 66: 59.
