Near-Infrared Reflectance Spectroscopy Analytic Models Established for the Crude Protein and Crude Fiber of Alfalfa Property
SHANG Chen1, ZHANG Yue-xue2*, LI Yan2, HAO Ruo-chao2, TANG Feng-lan2, HAN Wei-bo2, LIU Jie-lin2
1. Life Science and Technology Institute, Harbin Normal University, Harbin 150025, China 2. Institute of Pratacultural Sciences, Heilongjiang Academy of Agricultural Sciences, Harbin 150086, China
Abstract：Near-infrared reflectance spectroscopy (NIRS) calibrations of crude protein and crude fiber in 152 alfalfa samples were developed by means of partial least-squares (PLS) regression. Different interval wavelengths of 1, 2 and 5 nm and two kinds of samples (crude and fine) were set to collect spectral data and establish calibration respectively. The accuracy and repeatability of calibrations were verified so as to compare superiority and inferiority. Results showed that the best validation result is the calibration by 2 nm interval wavelength and fine sample, and the correlation coefficients of calibration (R2) were 0.97 and 0.94 for crude protein and crude fiber, and the SECV of these parameters were 0.42 and 0.78 respectively. The coefficients of determination for validation (R2val) were 0.96 and 0.92, and the SEP of these parameters were 0.43 and 0.79. The experiment showed that it is feasible to evaluate the major quality traits of alfalfa by NIRS. All together, it provided a new model to verify the crude protein and fiber compositions of alfalfa.
Key words：Near-infrared reflectance spectroscopy;Content;Alfalfa;Crude protein;Crude fiber;Partial least squares (PLS)
尚 晨1，张月学2*，李 炎2，蒿若超2，唐凤兰2，韩微波2，刘杰淋2 . 紫花苜蓿粗蛋白和粗纤维近红外分析模型的建立[J]. 光谱学与光谱分析, 2009, 29(12): 3250-3253.
SHANG Chen1, ZHANG Yue-xue2*, LI Yan2, HAO Ruo-chao2, TANG Feng-lan2, HAN Wei-bo2, LIU Jie-lin2 . Near-Infrared Reflectance Spectroscopy Analytic Models Established for the Crude Protein and Crude Fiber of Alfalfa Property . SPECTROSCOPY AND SPECTRAL ANALYSIS, 2009, 29(12): 3250-3253.
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