Abstract：In order to study the feasibility of using near infrared(NIR) diffuse reflectance spectroscopy to discriminate adulteration of non-ruminant meat and bone meal(MBM) with ruminant MBM,a total of 39 MBM samples made up of 15 from pig,15 from poultry,5 from cattle and 4 from sheep produced in different areas in China were chosen. The MBM samples were ground with 0.5 mm sieve. 252 specimens were prepared by non-ruminant MBM deliberately adulterated with different proportion of ruminant MBM. The specimens were scanned by FOSS NIRSystemTM 6500. A calibration set of 180 specimens and an independent validation set of 72 specimens were randomly selected by the WINISI software. Discriminant analysis model was developed by partial least squares (PLS) on the calibration set and validated with independent validation set. The best discriminant model was obtained using standard normal variate and detrend(SNVD) and second derivative for spectrum pretreatment; this model had a coefficient of determination (R2CV) of 0.83 and a standard error of cross-validation (SECV) of 0.147 1. For the independent validation set,the correct classification rate is 90%. There were a false negative specimen (0.5%) and two uncertain specimens (1%,1.5%) in validation set. Results showed that it is feasible to use NIR diffuse reflectance spectroscopy to discriminate adulteration of non-ruminant MBM with ruminant MBM,but for specimens adulterated with ruminant MBM at less than 2%,the accuracy of calibration model needs to be improved. NIR was a rapid and non-destructive approach to discriminating adulteration of non-ruminant MBM with ruminant MBM.
Key words：Meat and bone meal(MBM);Near-infrared diffuse reflectance spectroscopy(NIRDRS);Discriminant analysis;Partial least squares (PLS)
李琼飞,杨增玲,韩鲁佳*. 反刍动物源肉骨粉的近红外漫反射光谱判别分析[J]. 光谱学与光谱分析, 2008, 28(03): 572-577.
LI Qiong-fei,YANG Zeng-ling,HAN Lu-jia*. Discriminant Analysis of Near Infrared Diffuse Reflectance Spectra to Detect Adulteration of Non-Ruminant Meat and Bone Meal. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2008, 28(03): 572-577.
[1] CHEN Ying,WU Ya-jun,XU Bao-liang,et al(陈 颍，吴亚君，徐宝梁，等). Science and Technology of Food Industry,2004,25(8): 144. [2] ZHOU Jun-mei(周筠梅). Prog. Biochem. Biophys,2004,31(2): 95. [3] EC Bovine Spongiform Encephalopathy(BSE)，3rd Edn. Vademecum，European Community，Brussels，1998. [4] WANG Duo-jia，ZHOU Xiang-yang，JIN Tong-ming，et al(王多加，周向阳，金同铭,等). Spectroscopy and Spectral Analysis(光谱学与光谱分析)，2004，24(4)：447. [5] YING Yi-bin,LIU Yan-de,FU Xia-ping(应义斌，刘燕德，傅霞萍). Spectroscopy and Spectral Analysis(光谱学与光谱分析)，2006，26(1)：63. [6] CHU Xiao-li，YUAN Hong-fu，LU Wan-zhen(褚小立，袁洪福，陆婉珍). Spectroscopy and Spectral Analysis(光谱学与光谱分析)，2005，25(6)：886. [7] QIN Fang-li,MIN Shun-gen,LI Ning(覃方丽，闵顺根，李 宁). Spectroscopy and Spectral Analysis(光谱学与光谱分析)，2003，23(6)：1090. [8] QU Hai-bin，YANG Hai-lei，CHENG Yi-yu(瞿海斌，杨海雷，程翼宇). Spectroscopy and Spectral Analysis(光谱学与光谱分析)，2006，26(1)：60. [9] TANG Jun-ming,SUN Su-qin,YUAN Zi-min,et al(汤俊明，孙素琴，袁子民，等). Spectroscopy and Spectral Analysis(光谱学与光谱分析)，2004，24(5)：554. [10] Murray I，Aucott L S，Pike I H. Journal of Near Infrared Spectroscopy，2001，9: 297. [11] Murray I. Detection of Adulteration of Fishmeal & Feeds with Meat and Bone Meal(MBM) by Discriminant Analysis on Visible and NIR Spectra. The 18th Non-Destructive Detection Symposium，13-15,Nov.,2002，Tsukuba，Japan，2002. 185. [12] Fernndez M,Martínez A,Modroos,et al. Near Infrared Reflectance Spectroscopy as a Tool to Predict Qualitative and Quantitative Meat and Bone Meal Presence in Compound Feed. In: Davies A M C,Cho R K(Editors). Proceedings of the 10th International Conference. Near Infrared Spectroscopy. NIR Publications. Chichester,West Sussex (UK),2002. 307. [13] Perez-Marin Dolores C，Garrido-Varo Ana，Guerrero-Ginel J E，et al. Animal Feed Sci. and Tech.,2004,116: 333. [14] Perez-Marin Dolores C，Garrido-Varo Ana，Guerrero-Ginel J E，et al. Detection and Quantification of Mammalian Meat and Bone Meal in Compound Feedingstuffs by NIR Spectroscopy. In: Davies AMC,Garrido A. Proceedings of the 11th International Conference on Near-Infrared Spectroscopy，6-11,April,2003，Cordaba，Spain. Norfolk-UK，NIR Publications，2004. 667. [15] Termes S,Tauler R,Puigdomènech A. Preliminary Results in the Determination of Meat and Bone Meal in Feedingstuffs. In: Davies A M C,Garrido A(Editors). Near Infrared Spectroscopy. Proceedings of the 11th International Conference. NIR Publications. Chichester,West Sussex (UK),2004. 659. [16] STRATFEED“Strategies and Methods to Detect and Quantify Mammalian Tissues in Feedingstuffs”. EC Project No. G6RD-2000-CT00414，Growth Programme 5FWP. http://stratfeed.cra.wallonie.be. [17] Dardenne P，et al. Strategies and Methods to Detect and Quantify Mammalian Tissues in Feedingstuffs. Final Report of the STRATFEED Project. European Communities，Luxembourg，2005. WP5-NIRS. [18] WINISI Ⅱ Version 1.5 Manual. Foss-Tecator-Infrasoft International，Port Matilda PA，USA. [19] WU Jian-guo，SHI Chun-hai，ZHANG Hai-zhen(吴建国，石春海，张海珍). Spectroscopy and Spectral Analysis(光谱学与光谱分析)，2006，26(2)：259. [20] GAO Rong-qiang，FAN Shi-fu，YAN Yan-lu，et al(高荣强，范世福，严衍禄，等). Spectroscopy and Spectral Analysis(光谱学与光谱分析)，2004，24(12)：1563. [21] ZHENG Yong-mei，ZHANG Tie-qiang，ZHANG Jun,et al(郑咏梅，张铁强,张 军,等). Spectroscopy and Spectral Analysis(光谱学与光谱分析)，2004，24(12)：1546. [22] LIANG Yi-zeng，YU Ru-qin(梁逸曾，俞汝勤). Chemometrics(化学计量学). Beijing: Higher Education Press(北京：高等教育出版社)，2003. 219. [23] YAN Yan-lu，ZHAO Long-lian，HAN Dong-hai,et al(严衍禄，赵龙莲，韩东海,等). The Fundament and Application for Near-Infrared Spectra Analysis(近红外光谱分析基础与应用). Beijing: Chinese Light Industry Press(北京：中国轻工出版社)，2005. 29. [24] Ana Garrido-Varo，Dolores C,Perez-Marin，et al. Biotechnol. Agrom. Soc. Environ.,2005，9(1): 3. [25] Stefano Bellorini，Stefan Strathmann，Vincent Baeten，et al. Anal. Bioanal. Chem.,2005,382：1073.
