Determination of Trace Chromium(Ⅵ) by Catalytic Spectrofluorimetry Using Pyronine Y-H2O2-Cr(Ⅵ) System
LIN Shao-yu1, 2,GAO Wen-hua1*,LIU Xiao-xuan1,CHEN Yao-wen1
1. Department of Chemistry, Shantou University, Shantou 515063, China 2. Longhu Sub-Bureau, Shantou Environmental Protection Bureau, Shantou 515043, China
Abstract：A simple and highly sensitive new kinetic catalytic fluorimetric method was proposed for the determination of trace chromium (Ⅵ), based on the catalytic effect of trace amounts of chromium (Ⅵ) on the oxidation of Pyronine Y by hydrogen peroxide in acetic acid-sodium acetate buffer medium leading to a decrease in the fluorescence intensity. The optimum conditions and kinetic properties of the catalytic reaction were also studied. The apparent activation energy and the apparent rate constant are 159.92 kJ·mol-1 and 5.7×10-2 s-1 respectively. The linear range of the calibration curve is 0.02-0.24 μg·mL-1 and the detection limit is 0.012 μg·mL-1. The present method was applied to the determination of chromium(Ⅵ) in river water，and industrial and electroplating waste water with good results.
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