藻蓝蛋白原位还原Ag(I)与纳米Ag(0)形成动态过程的谱学研究

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摘要：在4 ℃避光条件下，将藻蓝蛋白(简称PC)与AgNO₃作用，通过UV，FS，FTIR等谱学方法研究了PC与Ag(I)原位还原与纳米Ag(0)粒子形成的动态过程。结果显示：PC在615 nm的特征吸收峰强度明显减弱，并随Ag(I)浓度增加和时间延长单调降低;PC的荧光发射峰和荧光激发峰也均呈现衰减趋势。同步荧光光谱观察到纳米Ag(0)粒子形成的动态过程。用TEM观察到所形成的Ag (0)分散于PC表面，形成PC为核，纳米银为壳部分包覆的生物缀合物，粒子呈球形，有较窄的分布尺寸，粒径在15～30 nm之间。

关键词：银;钝顶螺旋藻;藻蓝蛋白;光谱;纳米

Abstract：The interaction of Ag(I) and Phycocyanin(PC for short) was investigated by ultraviolet-visible, fluorescence and infrared spectra under the condition of 4 ℃ and without light. The results showed that the characteristic absorption of PC at 615 nm decreased gradually with the increase in Ag(I) concentration and interaction time. Accordingly, its fluorescence emission peak and two fluorescence excitation peaks also decreased. Dynamic formation of nano-Ag(0) was also observed by synchronous fluorescence spectra, showing that Ag(0) was formed by the PC in situ reduction. It was observed by TEM that Ag(0) dispersed in PC, which formed a bioconjugate with PC as the core and nano-Ag(0) as the crust. The bioconjugate was round and its diameter was about 15-30 nm.

Key words：Silver;Spirulina platensis;Phycocyanin;Spectroscopy;Nanoparticle

收稿日期: 2005-11-16 修订日期: 2006-03-06

中图分类号:

Q51

通讯作者: 郑文杰 E-mail: tzhwj@jnu.edu.cn

引用本文:

杨芳^1，2,郭振江¹,白燕¹,郑文杰^1，2* . 藻蓝蛋白原位还原Ag(I)与纳米Ag(0)形成动态过程的谱学研究[J]. 光谱学与光谱分析, 2007, 27(01): 23-27.
YANG Fang^{1, 2},GUO Zhen-jiang¹,BAI Yan¹,ZHENG Wen-jie^{1, 2*} . Spectra of in situ Reduction of Ag(I) by Phycocyanin and Dynamic Formation of Ag(0). SPECTROSCOPY AND SPECTRAL ANALYSIS, 2007, 27(01): 23-27.

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https://www.gpxygpfx.com/CN/Y2007/V27/I01/23

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