Study of Human Tumor Tissues by Raman Imaging Spectra
YU Ge1,ZHANG Pan2,TAN En-zhong1,ZHANG Cun-zhou2
1. Department of Mathematics and Physics, Beijing Institute of Petrochemical Technology, Beijing 102617, China 2. The Key Laboratory of Advanced Technique and Fabrication for Weak-Light Nonlinear Photonics Materials, Ministry of Education;TEDA Applied Physics College, Nankai University, Tianjin 300457, China
Abstract：The Raman mapping spectra were collected from the samples of fresh human breast tumor tissues with the excitation wavelength at 633 nm, their correlation coefficients with the reference spectra of chemical DNA, cytoplasm and extra-cellular matrix etc. were calculated, and the images were created based on these correlation coefficients. Examining these images, the authors found that they show approximately the distribution of the corresponding reference chemicals in human tissues and that the DNA spectrum correlation images reflect the DNA distribution quite well because of its much lower correlation with the spectra of other morphological chemicals. This work is helpful in understanding the chemical/morphological basis of the breast tissue Raman spectra and in developing the Raman diagnostic method for breast tumor.
