1. College of Science and Technology, Inner Mongolia University, Huhhot 010021, China 2. College of Water Conservancy and Civil Architecture Engineering, Inner Mongolia Agricultural University, Huhhot 010018, China
Abstract：After the HRP was treated with different strength of electric field, the effect of electric field on the secondary structure of HRP was studied by circular dichroism . The results show that different electric field strength ranging from 1.0 to 6.0 kV·cm-1 has a different effect on the relative contents of α-helix , β-sheet, β-turn and random coil of the HRP. As compared with the contrast, the relative contents of α-helix, β-turn and random coil decreased by 0.3%-11.4%, 1.7%-15.3% and 0.9%-20.4% respectively, while β-sheet content increased by 0.9%-82.7%. The electric treatment tends to transform the α-helix, β-turn and random coil into β-sheet for the HRP. The result of this study has important meaning to explain the biological effect of electric treatment seeds.
许强1,杨体强1*,敖敦格日勒1,姚占全2. 应用圆二色光谱研究电场对辣根过氧化物酶二级结构的影响[J]. 光谱学与光谱分析, 2007, 27(03): 565-568.
XU Qiang1,YANG Ti-qiang1*,AO Dun-ge-ri-le1,YAO Zhan-quan2 . Study on the Effect of Electric Field on the Secondary Structure of HRP by Circular Dichroism . SPECTROSCOPY AND SPECTRAL ANALYSIS, 2007, 27(03): 565-568.
[1] SUN Ke-li, XI Bao-shu, CAI Guo-you(孙克利，席保树，蔡国友). Acta Biophysica Sinica(生物物理学报), 1999, 15(3): 579. [2] Murr L E. Nature, 1963, 200: 490. [3] Gubbel G H. Can. J. Plant Sic., 1982, 62(1): 61. [4] Teramura A H, Ziska I H. Physiol. Plant, 1991, 83: 373. [5] Wu Lifang, Li Hong, Feng Huiyun. Chinese Science Bulletin, 2001, 46(4): 46. [6] WANG Xin，LI Su-hua，MIN Wei-hong, et al(王 莘，李肃华，闵伟红, 等). Acta Biophysica Sinica(生物物理学报)，1997，13(4): 665. [7] WANG Shu-hui，LI Xian-dong，SONG Chang-xian(王淑惠, 黎先栋, 宋长铣). Progress in Biochemistry and Biophysics(生物化学与生物物理进展)，1991, 18(5): 392. [8] ZHAO Jian，MA Fu-rong，YANG Wen-jie, et al(赵 剑，马福荣，杨文杰, 等). Acta Biophysica Sinica(生物物理学报)，1997，13(3)：489. [9] XU Yue-ying，ZHENG Rong，YANG Ti-qiang, et al(许月英, 征 荣, 杨体强, 等). Acta Scientiarum Naturalium Universitatis NeiMongol(内蒙古大学学报·自然科学版)，2003, 34(3): 294. [10] YANG Ti-qiang，LI Jin-mei，CHEN Yan, et al(杨体强，李金梅，陈 燕, 等). Acta Scientiarum Naturalium Universitatis NeiMongol(内蒙古大学学报·自然科学版)，1997, 28(6): 778. [11] YANG Ti-qiang，HOU Jian-hua，SU En-guang, et al(杨体强，侯建华，苏恩光, 等). Acta Biophysica Sinica(生物物理学报)，2000，16(4)：780. [12] LU Jian-gang，YANG Ti-qiang，SU En-guang, et al(吕剑刚，杨体强, 苏恩光, 等). Acta Scientiarum Naturalium Universitatis NeiMongol(内蒙古大学学报·自然科学版), 2001, 32(6): 707. [13] WEI Hong-en, XIAO Ji-zhong, ZHANG Qing-min(韦宏恩，肖吉中，张庆民). Acta Tabacaria Sinica(中国烟草学报), 1995, 12(4)：61. [14] O’brien P J. Chem. Biol. Interact, 2000, 129(1-2): 113. [15] Chattopadyay K, Mazumdar S. Biochemistry, 2000, 39(1): 263. [16] Gajhede M, Schuller D J, Henriken A, et al. Nat. Struct. Biol., 1997, 4: 1032. [17] Smulevich G, Paoli M, Burke J F, et al. Biochem.，1994, 33(23): 7398. [18] Welinder K G. Curr. Opin. Struct. Biol., 1992, 2: 388. [19] HONG Fa-shui(洪法水). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2003，23(3): 583. [20] Comara M J, Canto J, Alsina I, et al. Letters in Peptide Science, 2001, 7: 255. [21] Johnson W C. Proteins, 1999, 35(3): 307. [22] Sreerama N, Venyaminov S Y, Woody R W. Anal. Biochem.，2000, 287(2): 243. [23] Holzbaur I E, English A E, Ismail A A. Biochemistry, 1996, 35: 5488. [24] Krimm S, Bandekar J. Adv. Protein Chem., 1986, 38: 181. [25] Haris P I, Chapman D. Biopolymers (Peptide Sci.), 1995, 37: 251. [26] ZENG Zhong-kui, ZHANG Peng-fei, BAO Jin-ku, et al(曾仲奎，张鹏飞，鲍锦库, 等). Journal of Sichuan University(Natural Science Edition)(四川大学学报·自然科学版)，1997，34(4): 516. [27] Ho S Y, Mittal G S, Cross J D. J. Food Eng., 1997, 31: 69. [28] Vega-Mercado H, Powers J R, Barbosa-Cánovas G V, et al. Food Sci., 1995, 60(5): 1143. [29] XIA Bing-le, PENG Dun-geng, ZHENG Xiao-yun, et al(夏炳乐，彭敦耕，郑晓云，等). Acta Chimica Sinica(化学学报), 2004，62(14): 1318.
