绿色木霉纤维二糖水解酶Ⅱ的X射线辐射损伤研究

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摘要：根据半胱氨酸相关的拉曼光谱分析指标，采用X光管1.82 keV，40 mA，处理4 h的辐射条件，水溶液中绿色木霉纤维二糖水解酶Ⅱ的各种S—H伸缩振动拉曼谱带虽然偏移不多，但呈现了二极分化的演变状态。辐照后的样品中2 554 cm^-1峰宽加大。辐照没有引起维系高级空间结构存在的二硫键断裂，二硫键键长有缩小变化，二硫键几何构象类型未改变，但各种异构体成分的相对含量有变化，辐射前半胱氨酸C—S键异构体特征模P_C型略多，辐射后P_N和P_H型增多。除半胱氨酸CH₂摇摆的贡献在辐射后有明显减弱外，此辐射条件没有造成拉曼技术所能证明的与巯基有关的蛋白结构的明显损伤，但可见有变化存在。

关键词：辐射;纤维二糖水解酶Ⅱ;半胱氨酸;拉曼光谱

Abstract：After X-ray treatment at 1.82 keV and 40 mA and 4 hours, the cellobiohydrolase Ⅱ(CBHⅡ) aqueous solution of Trichoderma viride was analysed, and the damage state of the irradiated molecule was detected using some cysteine residue relative parameters in Raman spectroscopic methods. The results show that S—H stretch modes of CBHⅡ exhibited some shift, which means that the hydrogen proton donor state of sulfhydryl groups was stronger and weaker, respectively. The 2 554 cm^-1 peak of irradiated sample was wide. The —S—S— construction of disulfide bonds was not broken, and the geometrical conformation types did not change either, but its bond length was somewhat shortened. Before irradiation, C—S isomer mode content of cysteine residue was P_C type slightly more than both P_N and P_H types, while P_N and P_H types increased after irradiation. Besides, CH₂ rocking mode of cysteine residue was weakened remarkably after the treatement, and the protein molecule structure did not show important damage in sulfhydryl and disulfide bonds, but showed some change because of the X-ray irradiation condition.

Key words：Radiation;CBHⅡ;Cysteine;Raman spectroscopy

收稿日期: 2006-01-16 修订日期: 2006-04-28

中图分类号:

O657.3

通讯作者: 余多慰 E-mail: yuduowei@njnu.edu.cn

引用本文:

余多慰,袁生. 绿色木霉纤维二糖水解酶Ⅱ的X射线辐射损伤研究[J]. 光谱学与光谱分析, 2007, 27(04): 819-822.
YU Duo-wei, YUAN Sheng. Damage Analyse of X-Ray Radiated Cellobiohydrolase Ⅱ Molecule from Trichoderma Viride . SPECTROSCOPY AND SPECTRAL ANALYSIS, 2007, 27(04): 819-822.

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