1. College of Food Science & Technology, Huazhong Agricultural University, Wuhan 430070, China 2. Center for Fruit & Vegetable Processing Engineering Research, China Agricultural University, Beijing 100083, China
Abstract：The effect of pulsed electric field (PEF) on the secondary and tertiary structure of lipoxygenase (LOX) in a buffer solution was analyzed using far UV-circular dichroism (CD) and fluorescence spectrophotometry, respectively. The secondary and tertiary structure of LOX changed after PEF treatment. The CD spectra of LOX also changed, with the intensity of two negative peaks and the content of α-helix significantly decreased (p＜0.05). The decrease in α-helix content in LOX showed a good linear correlation with the electric field strength. The fluorescence intensity of LOX increased, and the relative fluorescence intensity of the two characteristic peaks of LOX emission spectra at 337 nm and 583 nm also showed a good linear correlation with the electric field strength. These results showed that the activity inactivation of LOX may be due to the alteration in secondary structure, and both had a good relation.
Key words：Pulsed electric field(PEF);Lipoxygenase (LOX);Conformation;Circular dichroism(CD);Fluorescence spectrum
钟葵1,2,胡小松2,吴继红2,陈芳2,廖小军2*. 高压脉冲电场对脂肪氧化酶二级和三级构象的影响效果[J]. 光谱学与光谱分析, 2009, 29(03): 765-768.
ZHONG Kui1,2,HU Xiao-song2,WU Ji-hong2,CHEN Fang2,LIAO Xiao-jun2* . Effects of High Pulsed Electric Field on the Secondary and Tertiary Structure of Lipoxygenase. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2009, 29(03): 765-768.
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