1. Chemistry Department of Northeast University, Shenyang 110004, China 2. College of Chemistry, Liaoning University, Shenyang 110036, China 3. Laser Biomedical Research Center, Shenyang Ligong University, Shenyang 110016, China
Abstract：The fluorescence spectra of protoporphyrin Ⅸ (PPⅨ) solutions and human serum samples were measured and analyzed under physiological conditions. The experimental results showed that the fluorescence of PP Ⅸ in human serum was mainly derived from PPⅨ-serum albumin complex. Moreover, the effects of serum albumin and PPⅨ on the PP Ⅸ emission fluorescence were also investigated. Compared with pure PPⅨ solution, not only a red-shift of PPⅨ fluorescence peak was found for PPⅨ+albumin solution, but also albumin had a fluorescence enhancement effect on protoporphyrin Ⅸ. For the mixture of PPⅨ+albumin, with the increase of PPⅨ the wavelength of the PPⅨ emission peak will increased a little when its concentration was less than 0.8×10-5 mol·L-1,but was nearly invariable when its concentration was more than 0.8×10-5 mol·L-1.
Key words：Protoporphyrin Ⅸ;Fluorescence;Human serum;PPⅨ serum albumin complex;Bovine serum albumin
[1] Kathryn S Kalasinsky, Victor F Kalasinsky. Spectrochimica Acta Part A, 2005, 61: 1707. [2] Masilamani V, Al-Zhrani K, Al-Salhi M. Journal of Luminescence, 2004, 109: 143. [3] Nina M Broer, Tim Liesenhoff, Hans-Henning Horch. Med. Laser Appl., 2004, 19: 45. [4] Giraev K M, Ashurbekov N A, Medzhidov R T. International Journal of Modern Physics B, 2004, 18(6): 899. [5] ZHANG De-yang, et al(张德阳, 等). China Medical Engineering(中国医学工程)，2004, 12(3): 102. [6] Lorenzo Brancaleon, Harry Moseley. Biophysical Chemistry，2002, 96: 77. [7] LIAN Shao-hui, et al(连少辉, 等). Chinese Biochemical Journal(生物化学杂志), 1991, 7(1): 36. [8] Markolf H Niemz. Laser-tissue Interaetion: Foundamentals and Applications. Springer-Verlag Berlin Heidelberg, 1996. 40. [9] YU Jia, et al(于 佳, 等). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2004, 24(8): 981. [10] MENG Ji-wu, et al(孟继武, 等). Acta Laser Biology Sinica(激光生物学报), 2004, 13(2): 88. [11] SHI Shuang-qun, et al(石双群, 等). Chemistry(化学通报), 2001, 64(6): 360.
