Abstract：The interaction between indomethacin (IM) and bovine serum albumin (BSA) under physiological condition was studied using fluorescence and absorption spectra. It was proved that fluorescence quenching of BSA by IM is deduced by combining static quenching with nonradiative energy transfer. The binding constants were obtained by fluorescence quenching method. The main sorts of binding force were determined according to the thermodynamic parameters. The binding distances and energy transfer efficiencies between indomethacin and bovine serum albumin were obtained based on the theory of Frster nonradiative energy transfer. Combining the results of UV absorption and fluorescence spectroscopic methods, the binding mode of IM with BSA was discussed.
曹玺珉,杜黎明*. 吲哚美辛与牛血清白蛋白结合作用的研究[J]. 光谱学与光谱分析, 2007, 27(05): 973-977.
CAO Xi-min,DU Li-ming*. Spectroscopic Study on Binding of Indomethacin to Bovine Serum Albumin. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2007, 27(05): 973-977.
[1] YU Tian-zhi, TAO Zu-yi(俞天智, 陶祖贻). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 1999, 19(3): 453. [2] Guo M, Zou J W, Yi P G, et al. Analytical Sciences, 2004, 20(3): 465. [3] Cui F L, Fan J, Li J P, et al. Bioorganic & Medicinal Chemistry, 2004, 12: 151. [4] CHEN Guo-zhen, HUANG Xian-zhi, ZHENG Zhu-zi, et al(陈国珍, 黄贤智, 郑朱梓，等). Methods of Fluorescence Analysis, 2nd Ed(荧光分析法·第2版). Beijing: Science Press(北京：科学出版社), 1990. 112. [5] Lakowica J R, Weber G. Biochemistry, 1973, 12(21): 4161. [6] Ware W R. J. Phys. Chem., 1962, 66(3): 455. [7] ZHANG Hai-rong, GUO Si-yuan, LI Lin, et al(张海容, 郭祀远, 李 琳，等). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2001, 21(6): 829. [8] BI Xin-ying, CHI Yan-hua(迟欣颖，迟燕华). Spectroscopy and Spectral Analysis(光谱学与光谱分析)，2006，26(11)：2097. [9] Klotz I M, Urquhart J M. J. Am. Chem. Soc., 1949, 71(3): 847. [10] Ross P D, Subramanian S. Biochem., 1981, 20(11)：3096. [11] YANG Man-man, YANG Pin, ZHANG Li-wei(杨曼曼, 杨 频, 张立伟). Chinese Science Bulletin(科学通报), 1994, 39(1): 31. [12] MA Chun-qi, LI Ke-an, et al(马春琪，李克安，等). Acta Chimica Sinica(化学学报), 1999, 57(4): 389. [13] Brustein E A, Vedenkina N S, Irkova M N. Photochem. Photobio., 1973, 18: 263. [14] Ulrich K H. Pharmacol. Rev., 1981, 33(1): 17. [15] MA Gui-bin, GAO Fei, et al(马贵斌，高 飞，等). Acta Chimica Sinica(化学学报), 1995, 53(12) : 1193. [16] Dockal M, Chang M, Carter D C, et al. Protein Sci., 2000, 9: 1455. [17] Vorum H, Honore B. J. Pharm. Pharmacol.，1996, 48(8): 870.
