1. College of Science, Agricultural University of Hebei, Baoding 071001, China 2. College of Vocational and Technological Sanitation, Hebei University, Baoding 071002, China
Abstract：The interaction between luteolin and human serum albumin (HSA) was studied by using fluorescence quenching spectra, synchronous fluorescence spectra and ultra-violet spectra. The results showed that luteolin had a strong ability to quench the fluorescence of HAS. The Stern-Volmer curve of the fluorescence quenching of HSA by luteolin indicated that the mechanism behind the quenching between luteolin and HSA was a static quenching. According to the Fōrster theory of non-radiation energy transfer, the binding distances (r) and the binding constants (KA) were calculated. The thermodynamic parameters showed that the interaction between luteolin and HSA was driven mainly by hydrophobic force. Synchronous spectra were used to investigate the conformational changes of HSA.
张志恒1,李彩瑞2,马晶军1,吴秋华1,王春1,仵茜1,王志1*. 荧光法研究木犀草素与人血清白蛋白的相互作用[J]. 光谱学与光谱分析, 2008, 28(05): 1135-1139.
ZHANG Zhi-heng1,LI Cai-rui2,MA Jing-jun1,WU Qiu-hua1,WANG Chun1,WU Qian1,WANG Zhi1*. Study on the Interaction of Luteolin with Human Serum Albumin by Fluorescence Quenching Method. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2008, 28(05): 1135-1139.
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