1. ESPC State Key Joint Laboratory, Department of Environmental Science and Engineering, Tsinghua University, Beijing 100084, China 2. Department of Chemistry, Tsinghua University, Beijing 100084, China
Abstract：The present paper investigated phosphorus forms in the extracellular polymeric substances (EPS) using 31P-NMR spectroscopy, and analyzed the influential parameters for this method. The results showed that in the three investigated EPS samples five P forms were identified, including orthophosphate, orthophosphate monoesters, DNA, pyrophosphate and polyphosphate. And in the EPS extracted from A/A/O sludge and A/A/O-MBR sludge polyphosphate was the main phosphorus form. This indicated that P is contained in the EPS not only owing to the adsorption of orthophosphate, but also because EPS acting like the bacteria cells contributes to enhanced biological phosphorus removal. Furthermore the transformation of P forms in the EPS during the EPS extraction was found but limited within 1 h. It was found that with the NaOH solution added the polyphosphate in the EPS would partly convert to pyrophosphate but not go further to orthophosphate, while with pure water added P forms in the EPS were very stable. This means that after neutralization, the transformation of phosphorus forms could be avoided. Since the measurement of 31P-NMR spectroscopy was very sensitive to pH, which could affect the chemical shift of the peaks and cause peaks overlapping, the pH of EPS sample must be raised to 13.0 before the measurement.
Key words：31P-NMR spectroscopy;Extracellular polymeric substances;Biological phosphorus removal;Phosphorus form
张志超1,黄霞1*,杨海军2,肖康1,罗小2,沙恒1,陈奕名1 . 生物除磷污泥胞外多聚物含磷形态的核磁共振分析[J]. 光谱学与光谱分析, 2009, 29(02): 536-539.
ZHANG Zhi-chao1,HUANG Xia1*,YANG Hai-jun2,XIAO Kang1,LUO Xiao2,SHA Heng1,CHEN Yi-ming1 . Study on P Forms in Extracellular Polymeric Substances in Enhanced Biological Phosphorus Removal Sludge by 31P-NMR Spectroscopy. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2009, 29(02): 536-539.
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