Abstract：Two novel N-solanesylpiperazine triamines (4a,4b) were synthesized and their structures were characterized by 1H NMR,IR,MS,and elemental analysis. The preliminary in vitro tests revealed that the IC50 values of compound 4a and 4b on L1210 were 4.3 and 3.1 μmol respectively. The interaction of 4b with DNA was also investigated via UV and FL spectra with N1-(4-aminobutyl)-N4-(9-anthracenylmethyl) butane-1,4-diamine (5) as the reference compound. The results revealed that compound 5 could quench the fluorescence of DNA-EB system,while compound 4b had the opposite function. Further tests proved that the fluorescence of compound 5 and 4b could be both quenched by DNA. It was speculated that compound 4b,with the long chain and two tertiary nitrogens,interacted with DNA by the static attraction or was partially embedded into DNA,while compound 5 was typically inserted into DNA.
王建红,程鹏飞,孙心齐,赵瑾,王超杰* . N-(茄呢基哌嗪烷基)蒽甲胺的合成及其与DNA作用的研究[J]. 光谱学与光谱分析, 2007, 27(07): 1398-1402.
WANG Jian-hong,CHENG Peng-fei,SUN Xin-qi,ZHAO Jin,WANG Chao-jie*. Synthesis of N-(Solanesylpiperazine-Alkyl)-9-Anthracenemethylamines and Its Interaction with DNA. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2007, 27(07): 1398-1402.
[1] Casero J R A,Woster P M. J. Med. Chem.,2001,44: 1. [2] Karigiannis G,Papaioannou D. Eur. J. Org. Chem.,2000,10: 1841. [3] WANG Jian-lin,FU Lian-chun,ZHOU Shi-wu,et al(王建林，付连春，周实武，等). Spectroscopy and Spectral Analysis(光谱学与光谱分析),2005,25(6): 912. [4] DU Jiang-yan,HUANG Xiao-hua,XU Fei,et al(杜江燕，黄晓华，徐 飞，等). Spectroscopy and Spectral Analysis(光谱学与光谱分析),2005,25(9): 1435. [5] YANG Ming(杨 铭). Journal of Beijing Medical University(北京医科大学学报)，1998，30(2)： 97. [6] Brissault B,Kichler A,Guis C,et al. Bioconjugate Chem.,2003,14: 581. [7] Andrew J Geall,Blagbrough I S. Tetrahedron,2000,56: 2449. [8] Sidorova T A,Nigmatov A G,Kakpakova E S. J. Med. Chem.,2002,45: 5330. [9] Wang C J,Delcros J G,Biggerstaff J,et al. J. Med. Chem.,2003,46: 2663. [10] WANG Chao-jie,SONG Jin-yong,ZHAO Jin(王超杰,宋金勇,赵 瑾). Chin. J. Org. Chem.(有机化学),2004,24: 1444. [11] PENG Jun-feng,LING Jian-ya,ZHANG Han-xing,et al(彭俊峰，凌建亚，张晗星，等). Spectroscopy and Spectral Analysis(光谱学与光谱分析),2004,24(7): 858. [12] HE Ji-xiang,JIANG Chong-qiu,GAO Ming-xia(贺吉香，江崇球，高明霞). Spectroscopy and Spectral Analysis(光谱学与光谱分析),2003,23(4): 755.
