1. College of Animal Science and Technology,China Agricultural University,Beijing 100094,China 2. National Engineering Research Center for Information Technology in Agriculture,Beijing 100089,China 3. College of Science,China Agricultural University,Beijing 100094,China 4. Thermal Electron Technologies and Instruments Company,Beijing 100032,China
Abstract:It is very important to evaluate the fresh sample of alfalfa silage using near infrared reflectance spectroscopy technology (NIRS) for animal production. The nutrient content of forage means the contents of dry matter (DM),crude protein (CP),neutral detergent fiber (NDF),and acid detergent fiber (ADF) in the forage. Because of the high moisture content,it is difficult to make uniform samples for fresh forage and to get useful information from the spectrum. Therefore,it is hard to use NIRS analysis. In order to evaluate the feasibility of using NIRS to analyse the fresh alfalfa silage,the DM,CP,NDF and ADF contents of fresh alfalfa silage were evaluated by the near infrared reflectance spectroscopy model in this experiment using partial least square regression (PLS),Fourier transform technology and sample preparation with liquid nitrogen technology. The analysis samples were obtained through different cultivars,maturity,cuttings and ensiling method. The cross validation was determined between 0.884 6-0.989 8. The standard error of cross validation was between 3.9 and 9.7 g·kg-1 fresh weight. Fifty samples were used to test the performance of the models. The coefficients of correlation between the chemical value and the NIRS value are between 0.939 7 and 0.994 9,and the root mean square errors of prediction are between 1.9 and 8.3 g·kg-1 fresh weight. The results showed that NIRS could be used to evaluate the nutrition of fresh forage.
[1] Shin C N,Martin N P. Journal of the Korean Society of Grassland Science,1989,9(3): 163. [2] Martin N P,Marten G C,Schriever D A,et al. Proceedings of the XV International Grassland Congress,August 24-31,1985,Kyoto,Japan. Science Council of Japan and Japanese Society of Grassland Science,Nishi-Nasuno,Tochigi,Japan: 1985. 965. [3] Owens V N,Albrecht K A,Hintz R W. Journal of Production Agriculture(USA),1995,8(4): 491. [4] BAI Qi-lin,CHEN Shao-jiang,DONG Xiao-ling,et al(白琪林,陈绍江,董晓玲,等). Spectroscopy and Spectral Analysis(光谱学与光谱分析),2004,24(11): 1345. [5] BAI Qi-lin,CHEN Shao-jiang,DONG Xiao-ling,et al(白琪林,陈绍江,董晓玲,等). Spectroscopy and Spectral Analysis(光谱学与光谱分析),2006,26(2): 271. [6] Reeves J B,Blosser T H. J. Dairy Sci.,1989,72: 79. [7] Park R S,Agnew R E,Gordon F J,et al. Animal Feed Science and Technology,1998,72: 155. [8] Cozzolino D,Labandera M. Journal of the Science of Food and Agriculture,2002,82: 380. [9] Shenk J S,Harpster H W. J. Dairy Sci.,1988,71: 1955. [10] Muck R E. Transactions of the American Society of Agricultural Engineering,1987,30(1): 7. [11] YANG Sheng(杨 胜). Analysis of Feed and Assay Methodology of Feed Quality(饲料分析及饲料质量检测技术). Beijing: Beijing Agricultural University Press(北京: 北京农业大学出版社),1993. 58. [12] Association of Official Analytical Chemists. 1980,Official Methods of Analysis 13th ed. Assoc. Office Anal. Chem.,Washington,D C. [13] Sinnaeve G,Dardene P,Agneessens R,et al. J. Near Infrared Spectroscopy,1994,2: 79.