Study on the Effect of Electric Field on the Secondary Structure of SOD by FTIR Spectroscopy
DENG Yi-bing1,YANG Ti-qiang2
1. Marine Science College of Zhejiang Ocean University,Zhoushan 316004,China 2. College of Science and Technology,Inner Mongolia University,Huhhot 010021,China
Abstract：After the SOD (superoxide dismutase) was treated with different strength of electric field,the effect of electric field on the secondary structure of SOD was studied by FTIR (Fourier-transformation infrared spectroscopy). The results have shown that different electric field strength has different effects on the secondary structure contents of the SOD. As compared with the contrast,the relative contents of α-helix and β-sheet were decreased,while β-turn content increased. The random coil contents were increased except for the 1.0 kV·cm-1. The electric treatment tends to transform the α-helix and β-sheet into β-turn and random coil,and different strength of electric field has a different effect on the transformation. The changes in SOD activity have a direct relationship to the contents of β-sheet.
邓一兵1,杨体强2. 应用红外光谱研究电场对超氧化物歧化酶二级结构的影响[J]. 光谱学与光谱分析, 2007, 27(07): 1312-1315.
DENG Yi-bing1,YANG Ti-qiang2. Study on the Effect of Electric Field on the Secondary Structure of SOD by FTIR Spectroscopy. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2007, 27(07): 1312-1315.
