Oil Cells Distribution on Different Parts of Melaleuca Alternifolia and Its Research by Micro-Raman Spectroscopy
SI Min-zhen1, 2, LI Jia-wang1, 2, YANG Yong-an1, 2, ZHANG De-qing1, 2, LI Lun1, 2, ZHANG Chuan-yun1, 2
1. Key Laboratory of Molecular Spectroscopy, Colleges and Universities in Yunnan Province, Chuxiong Normal University, Chuxiong 675000, China
2. Application Institute of Spectroscopy Technology, Chuxiong Normal University, Chuxiong 675000, China
Abstract：In order to identify the ingredient in Melaleuca alternifolia oil cells at room temperature, and avoid sample pretreatment and extractions, which can be labour-intensive. Oil Cells Distribution on Different Parts of Melaleuca alternifolia and the principal component in oil cells have been studied by Micro-Raman Spectrometer. It has been found that there are barely any oil cells on the soft branches. There are more oil cells on new leaves compared to mature leaves. In Raman spectroscopy of mature leaf of oil cells, 1 675/726 cm-1（C═C stretching/ring deformation） are a characteristic key band of terpinene-4-ol, 1 700/754 cm-1（C═C stretching/ ring deformation） for γ-terpinene, 1 609 cm-1（C═C stretching）for α- terpinene and 1 522/1 156/1 011 cm-1（C═C stretching/C—C stretching/C—C in-plane rocking）for β-carotene. In Raman spectroscopy of new leaves oil cells, 745 cm-1 (ring deformation) is a characteristic key band of cis-sabinene hydrate, 1 609 cm-1 for α- terpinene and 1 522/1 160/1 008 cm-1 for β-carotene. The principal components are different in oil cells of new and mature leaves. It is the first report that there are cis-sabinene hydrate and β-carotene in oil cells of Melaleuca alternifolia. This method can be used for quality control and developmental research for Melaleuca alternifolia plant essential oil extraction.
Key words：Melaleuca alternifolia; Different parts; Oil cells; Raman spectrum
司民真，李家旺，杨永安，张德清，李 伦，张川云. 互叶白千层不同部位油细胞分布及显微拉曼光谱研究[J]. 光谱学与光谱分析, 2021, 41(03): 813-816.
SI Min-zhen, LI Jia-wang, YANG Yong-an, ZHANG De-qing, LI Lun, ZHANG Chuan-yun. Oil Cells Distribution on Different Parts of Melaleuca Alternifolia and Its Research by Micro-Raman Spectroscopy. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2021, 41(03): 813-816.
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