Abstract：Small biomolecules in the human body have important effects on the health of the living body. Some of them are involved in the blood circulation and immune system of the living body Therefore, the detection of small molecules in living organisms in the study of small molecules in the physiological function of life is of great significance. Fourier Transform Surface Plasmon Resonance (FT-SPR) spectroscopy has been widely used in molecular detection in recent years due to its advantages of simple operation, high sensitivity, few samples required and real-time detection But for different molecules, especially those with very small molecular weight, FT-SPR direct detection has some disadvantages, such as unstable signal and low sensitivity. Therefore, a series of SPR small molecule sensors can be constructed for different detection targets to remedy the above shortcomings. In this paper, the research progress in the detection of small molecules by SPR technology is reviewed from the aspects of immunosensor (direct detection, Sandwich detection, competitive detection and inhibition detection), SPR molecular imprinting sensor and other SPR based sensors. The prospect of its development is also given.
Key words：Surface plasmon resonance technology; Small molecule detection: SPR sensor
[1] DONG Mei-hua, SUN Shi-mei, JIN Biao（董美花，孙士美，金 彪）. Chinese Journal of Analytical Chemistry(分析化学), 2014, 42(9): 1235.
[2] WU Jia-rui（吴家睿）. Science（科学）, 2005, 57：28.
[3] Liu X M, Wang L, Cui H R, et al. Canadian Journal of Forest Research, 2019, 49：1147.
[4] Wang M M, Qu H B, Zhang H D, et al. Plant Molecular Biology, 2019, 99：461.
[5] Song X, Yue X, Chen W, et al. Frontiers in Plant Science, 2019, 10：798.
[6] LI Fei-fei, LU Yi-song, YANG Sheng-yuan, et al（李菲菲, 陆一松, 杨胜园，等）. Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2019, 39(9)：2769.
[7] LIU Ping, XIN Xian-liang，GENG Mei-yu（刘 萍，辛现良，耿美玉）. Advances in Modern Biomedicine（现代生物医学进展）, 2009, 9：1749.
[8] Wang Jing, Song Daqian, Wang Liying, et al. Sensors and Actuators B: Chemical, 2011, 157：547.
[9] Mohammadzadeh-Asl S, Keshtkar A, Ezzati Nazhad Dolatabadi J，et al. Biosensors and Bioelectronics, 2018, 110：118.
[10] GAO Zhi-xian，LIU Ming（高志贤, 柳 明）. International Journal of Biomedical Engineering（国际生物医学工程杂志），2010，33(1)：1.
[11] Kausaite-Minkstimiene Asta，Ramanaviciene Almira，Ramanavicius Arunas. Analyst, 2009, 134: 2051.
[12] Wang S, Dong Y, Liang X. Biosensors and Bioelectronics, 2018, 109：1.
[13] Abdullah S, Azeman N H, Mobarak N N, et al. Optik, 2018, 168：784.
[14] Dong Haibin, Zou Fei, Hu Xiaojun, et al. Biosensors and Bioelectronics, 2018, 117：605.
[15] Yao G H, Liang R P, Huang C F, et al. Analytica Chimica Acta, 2015, 871：28.
[16] Shim Y Y, Reaney M J T. Journal of Agricultural and Food Chemistry, 2015, 63：1099.
[17] Laia Reverté，Pablo de la Iglesia, Vanessa del Río, et al. Analytical Chemistry, 2015, 87：10839.
[18] Urusov A E, Kostenko S N, Sveshnikov P G, et al. Sensors and Actuators B: Chemical, 2011, 156：343.
[19] Liu Y, Wilson W. Drug-DNA Interaction Protocols. Methods in Molecular Biology, 2010, 613：1.
[20] Kun J, Khaywah M Y, Yugang L, et al. Acs Applied Materials & Interfaces, 2013, 6：219.
[21] Hua Z, Song D, Shang G, et al. Sensors & Actuators B Chemical, 2013, 188：548.
[22] Tomassetti M, Conta G, Campanella L, et al. Biosensors, 2016, 6: 22.
[23] Okumura A, Sato Y, Kyo M，et al. Analytical Biochemistry, 2005, 339：328.
[24] Melaine F, Roupioz Y, Buhot A. Procedia Technology, 2017, 27：6.
[25] Li Qing, Wang Qing, Yang Xiaohai, et al. Talanta, 2017, 174：521.
[26] Zeidan E, Shivaji R, Henrich V，et al. Scientific Reports，2016, 6: 26714.
[27] Hu W, He G, Zhang H, et al. Analytical Chemistry, 2014, 86(9)：4488.
[28] Jiang X Q, Waterland M, Blackwell L，et al. Analytical Methods, 2010, 2: 368.
[29] Sun R C, Chen Y, Hu X. Advanced Materials Research，2012, 442: 119.
[30] Cao Y, McDermott M T. Analytical Biochemistry, 2018, 557：7.
[31] LI Guang, CHEN Long-cong, LI Xian，et al(李 广，陈龙聪，李 贤，等). Sensors and Microsystems（传感器与微系统）, 2014, 156：144.
[32] Chen S H, Cui X Q, Yang F, et al. Chemical Research in Chinese Universities，2003, 249(10): 1770.
[33] Cao Y, Griffith B, Bhomkar P, et al. Analyst，2017, 143(1): 289.
[34] Moon J, Byun J, Kim H, et al. Sensors，2018, 18(2): 598.
[35] Luo Zewei, Zhang Ji, Wang Yimin, et al. Sensors and Actuators B: Chemical, 2016, 236：474.
[36] Yockell-Leliever H. Analyst, 2015, 140: 5105.
[37] Kawazumi H, Gobi K V, Ogino K, et al. Sensors and Actuators B: Chemical, 2005, 108：791.
[38] de-los-Santos-álvarez N, Lobo-Castañón M J, Miranda-Ordieres A J，et al. TrAC Trends in Analytical Chemistry, 2008, 27：437.
[39] SHI Rui-xue, GUO Cheng-hai, ZOU Xiao-hong, et al（史瑞雪，郭成海，邹小红，等）. Chemical Advances（化学进展）, 2002, 14：182.
[40] Jun M. Analytical Chemistry, 2005, 77(13): 4282.
[41] Yola M L, Atar N, Eren T. Sensors and Actuators B: Chemical, 2014, 198：70.
[42] Zhang Lili, Zhu Cuicui, Chen Changbao, et al. Food Chemistry, 2018, 266：170.
[43] Göçenoğlu Sarıkaya A, Osman B, Çam T，et al. Sensors and Actuators B: Chemical, 2017, 251：763.
[44] Jiang Suiyi, Peng Yuan, Ning Baoan, et al. Sensors and Actuators B: Chemical, 2015, 221：15.
[45] Tan Y, Ahmad I, Wei T X. Chinese Chemical Letters, 2015, 26：797.
[46] Stefan Schmieder, Janek Weißpflog, Norbert Danz, et al. Engineering in Life Sciences，2017, 17: 1264.
[47] Kyaw H, Boonruang S, Mohammed W，et al. AIP Advances，2015, 5(10): 107226.
[48] Patil S, Sistla S, Jadhav J. International Journal of Biological Macromolecules, 2016, 92：1123.
[49] Wang J, Wang F, Zou X, et al. Electrochemistry Communications, 2007, 9：343.
