Determination of Rutin by Flow Injection Chemiluminescence Method
LI Li-jun1,ZHONG Zhao-heng2,FENG Jun2,CHEN Qi-feng2,KONG Hong-xing1,WU Jian-ling2
1. Department of Biological and Chemical Engineering,Guangxi University of Technology,Liuzhou 545006,China 2. College of Chemistry and Chemical Engineering,Guangxi University,Nanning 530004,China
Abstract：A new flow injection chemiluminescence method for the determination of rutin was established. The method was based on the chemiluminescence reaction of rutin with cerium (Ⅳ) sulfate in sulfuric acid condition sensitized by rhodamine 6G. Various factors affecting the chemiluminescence intensity of the system were investigated,and the condition of sulfuric acid,cerium (Ⅳ) sulfate and Rhodamine 6G etc was optimized.We have done research on all kinds of possible disturbers in the sample. The possible mechanism of the proposed method was also studied. The method is simple,convenient and sensitive. The linear relationship between the intensity of chemiluminescence and the concentration of rutin is over the range of 1.0×10-7-1.0×10-4 mol·L-1. The detection limit is 8.1×10-8 mol·L-1,the relative standard deviation is 1.5% for 11 measurements of 1.0×10-5 mol·L-1 standard solution. This method has been successfully applied to the determination of rutin in tablets with recoveries between 99.0% and 104.0%.
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