Analysis of Acanthopanax Senticosus Harms for Different Parts Using Fourier Transform Infrared Spectroscopy
JIN Zhe-xiong1, XU Sheng-yan1, SUN Su-qin2*, ZHOU Qun2
1. Pharmic College of Harbin University of Commerce, Harbin 150076，China 2. Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology (Ministry of Education), Department of Chemistry, Tsinghua University, Beijing 100084, China
Abstract：In the present paper, raw radix, stem and leaf and the total glycoside extract of them of Acanthopanax senticosus Harms were studied via the multi-steps IR macro-fingerprint method. The spectra of raw medicinal materials show that the spectra of radix and stem are similar and mainly show characteristic peaks of calcium oxalate and starch, whereas the characteristic peaks of calcium oxalate of leaf almost disappear and the shape of characteristic peak of starch also gets unconspicuous. The FTIR spectra of total glycoside extract of radix, stem and leaf present characteristic peaks at 1 602, 1 514, 1 452 cm-1 (vibration of phenyl framework) and 1 271 cm-1(CO), respectively, therefore, the authors speculated that their mutual component is the compound of phenolic glycoside. Through observing the second derivative IR spectra of the total glycoside extract of different parts of Acanthopanax senticosus Harms, the authors found that the characteristic peak of leaf is stronger than that of radix and stem at 1 656 cm-1 (flavone CO), this proves that the quantity of component of flavone in the leaf is higher than that in the radix and stem. In the two-dimensional correlation spectra, the radix and stem both have five automatic peaks (vibration of phenyl framework) in 1 350-1 700 cm-1, whereas the leaf still shows another automatic peak at 1 656 cm-1 (flavone CO),and this further proves that the quantity of component of flavone in the leaf is higher than that in the radix and stem.
Key words：Infrared spectroscopy;Second derivative IR spectroscopy;Two-dimensional IR correlation spectroscopy;Acanthopanax senticosus Harms
金哲雄1,徐胜艳1,孙素琴2*,周群2. 刺五加不同部位的红外光谱分析与鉴定[J]. 光谱学与光谱分析, 2008, 28(12): 2859-2863.
JIN Zhe-xiong1, XU Sheng-yan1, SUN Su-qin2*, ZHOU Qun2. Analysis of Acanthopanax Senticosus Harms for Different Parts Using Fourier Transform Infrared Spectroscopy. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2008, 28(12): 2859-2863.
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