基于四甲基联苯胺和吖啶橙间荧光共振能量转移的比率型荧光测定左氧氟沙星

翟好英, 赵文林, 周文俊^*

Ratiometric Fluorescence Determination of Levofloxacin Based on Fluorescence Resonance Energy Transfer Between Tetramethylbenzidine and Acridine Orange

ZHAI Hao-ying, ZHAO Wen-lin, ZHOU Wen-jun^*

(a) TMB的荧光光谱( λ _ex =310 nm) ( a ), AO的吸收光谱( b ), 发射光谱( λ _ex=405 nm) ( c )和( λ _ex=505 nm)( d ), LVF的吸收光谱( e )和发射光谱( λ _ex =390 nm)( f ); AO浓度(b)和TMB浓度(c)对FRET体系的影响 2.2 TMB荧光量子产率的检测