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2006 Vol. 26, No. 09
Published: 2006-09-26
 
       光谱学与光谱分析
1569 Using Instantaneous Spectra to Determine Dominant Species in the DDT Process of Epoxypropane
LI Ping1, 2, HU Dong2, 3, YUAN Chang-ying2, 4, DAI Song-hui1, XIAO Hai-bo1
After solving problems of weak light detection, the calibration of the spectral sensitivity of the measuring system, and the synchronization of the measuring system, instantaneous emission spectra of epoxypropane in the process of deflagration to detonation transition (DDT) with the exposure time of 2-8 μs and the resolution of 0.2 nm were acquired from six different side windows of an explosion shock tube. Using the corrected spectral data, curves of the optical radiant intensity of main reaction products versus the DDT distance from the ignition point were obtained. These curves provided information about the evolution of the reaction and the products during the DDT process. Results indicate that the chemical reaction rate of the gaseous fuel and the corresponding concentrations of intermediate products increased gradually at the deflagration stage, but at the moment of deflagration to detonation transition, the reaction rate increased rapidly and the concentrations!of products increased sharply. Among these main products, concentration increments of molecule CO, and radicals CHO and OH were greater than other products, which means that CO, CHO and OH are the dominant species that affect the DDT process greatly.
2006 Vol. 26 (09): 1569-1572 [Abstract] ( 2363 ) PDF (628 KB)  ( 384 )
1573 Experimental Study of Soft X-Ray Radiation in the Interaction of Circularly Polarized Femtosecond-Laser-Pulse with Low Pressure Xenon
WANG Xiang-xian1, 2，3,HUANG Wen-zhong2,GU Yu-qiu2,HONG Wei2,JIANG Gang3,WEN Xian-lun2,HE Ying-ling2,JIAO Chun-ye2,WANG Guang-chang2，3,ZHANG Shuang-gen2，3
Using flat-field grating Spectrometer, the ions lines with wavelength between 5 and 60 nm were measured, which were produced by the interaction of circularly polarized 35 femtosecond ultraintense and ultrashort laser-pulse with 5 mm length xenon at the pressure 2 and 3 kPa respectively. The highest transition is the XeⅧ：4d105s(2S1/2)—4d95s5p(2P3/2) line at wavelength 17.085 6 nm at 2 kPa and 3 kPa, the highest transition is 11.343 nm line of XeⅦ 4d105s2(1S0)—4d95s25f(3P1) transition. The xenon is ionized to XeⅦ，XeⅧ and XeⅨ at both pressure.
2006 Vol. 26 (09): 1573-1576 [Abstract] ( 323 ) PDF (410 KB)  ( 396 )
1577 Energy-Pooling Collisions of Rubidium Atoms: Rb(5PJ)+Rb(5PJ)→ Rb(5S)+Rb(nl=5D,7S)
MU Bao-xia,WANG Shu-ying,CUI Xiu-hua,ZHANG Gang-tai,YUAN Qiang-hua,DAI Kang,SHEN Yi-fan*
An experimental study of rubidium energy pooling collisions, Rb(5PJ)+Rb(5PJ)→Rb(nlJ′)+Rb(5S), at thermal energies, was carried out in a cell. Atoms were excited to either the 5P1/2 or 5P3/2 state using a single-mode diode laser. The excited atom density and spatial distribution were mapped by monitoring the absorption of a counter-propagating single-mode diode laser beam, tuned to either 5P1/2→5D3/2 or 5P3/2→7S1/2 transition, which could be translated parallel to the pump beam. The excited atom densities were combined with the measured fluorescence ratios to determine cross sections for the rubidium energy pooling process. For 5P3/2 excitation the cross sections for nlJ′ being 5D5/2, 5D3/2, and 7S1/2 are (1.32±0.59)×10-14, (1.18±0.53)×10-14 and (3.21±1.44)×10-15 cm2, respectively. For 5P1/2 excitation the cross sections for nlJ′ being 5D5/2 and 5D3/2 are (6.57±2.96)×10-15, and (5.90±2.66)×10-15 cm2, respectively. The results were compared with those of other experiments.
2006 Vol. 26 (09): 1577-1580 [Abstract] ( 1832 ) PDF (380 KB)  ( 443 )
1581 Photoelectron Decay Properties of Doped AgCl Microcrystals under Chemical Sensitization
LI Xiao-wei, JIANG Xiao-li, MENG Tao, DAI Xiu-hong, ZHAO Xiao-hui, DONG Guo-yi, HAN Li
The photoelectron decay characteristic directly reflects the photographic efficiency of silver halide crystals. Measurement of the electronic decay time-resolved spectrum of silver halide microcrystals can provide important information about the photoelectron decay action in latent image formation process. In order to know the influence of shallow electron trap dopant K4Fe(CN)6 and S+Au on photoelectron decay, the photoelectron decay time-resolved spectra of AgCl emulsion doped by K4Fe(CN) and that doped by K4Fe(CN) firstly and then sensitized by S+Au were detected by microwave absorption dielectric technique, which can be used to study the decay process of free photoelectrons and shallow-trapped electrons in semiconductor crystals. The experimental results show that when the doping content is 10-８-10-7 mol·mol-1Ag, the photoelectron decay process becomes slower, namely, the photoelectron decay time is longer, as the doping is near the grain surface before sensitization. After S+Au sensitization, the photoelectron decay becomes faster, showing that the sensitization centre acts as a deep electron trap. And when the doping is near the grain surface with 90% Ag, the photoelectron decay time becomes shorter, showing that the doping centre and the sensitization centre may interact.
2006 Vol. 26 (09): 1581-1583 [Abstract] ( 1649 ) PDF (337 KB)  ( 398 )
1584 Retrieval of Monocyclic Aromatic Hydrocarbons with Differential Optical Absorption Spectroscopy
XIE Pin-hua1, FU Qiang2, LIU Jian-guo1, LIU Wen-qing1, QIN Min1, LI Ang1, LIU Shi-sheng1, WEI Qing-nong1
Differential optical absorption spectroscopy (DOAS) technique has been used to measure trace gases in the atmosphere by their strongly structured absorption of radiation in the UV and visible spectral range, e.g. SO2, NO2, O3 etc. However, unlike the absorption spectra of SO2 and NO2, the analysis of aromatic compounds is difficult and strongly suffers from the cross interference of other absorbers (Herzberg bands of oxygen, ozone and sulfur dioxide), especially with relatively low concentrations of aromatic compounds in the atmosphere. In the present paper, the DOAS evaluation of aromatic compounds was performed by nonlinear least square fit with two interpolated oxygen optical density spectra at different path lengths and reference spectra of ozone at different temperature and SO2 cross section to correct the interference from absorbers of O2, O3 and SO2. The measurement of toluene, benzene, (m,p,o)xylene and phenol with a DOAS system showed that DOAS method is suitable for monocyclic aromatic compounds monitoring in the atmosphere.
2006 Vol. 26 (09): 1584-1588 [Abstract] ( 1698 ) PDF (569 KB)  ( 448 )
1589 Spectroscopic Measurements of Atomic Hydrogen Concentrations in Dielectric Barrier Discharge Hydrogen Plasmas
WANG Wei-guo1, XU Yong1, 2*, LIU Zhong-wei1, ZHU Ai-min1, 2, WANG Wen-chun1, 2
Atomic hydrogen plays important roles in chemical vapor deposition of functional materials, plasma etching，and surface cleaning. The present work introduces the fundamental principle to determine atomic hydrogen density via optical emission spectroscopy using Ar as an actinometer, and also reports the experimental results of atomic hydrogen density in the DBD discharge hydrogen plasmas. The variations of atomic hydrogen density and the hydrogen dissociation fraction as a function of pressure were calculated based on some of the available electron-impact excitation cross section and quenching cross sections in the literatures. In this work, as the pressure increases from 0.32 to 5.1 kPa, the hydrogen dissociation fraction decreases from 5.2% to 0.089%, and the atomic hydrogen density decreases from 4.9×1015 cm-3 to 1.3×1015 cm-3. The variations of H atom Balmer lines and Ar(750.4 nm) emission intensity as functions of gas pressure, discharge voltage, and frequency were also investigated.
2006 Vol. 26 (09): 1589-1593 [Abstract] ( 1206 ) PDF (615 KB)  ( 485 )
1594 Study on Concentration Modulation Spectra of the Molecular Ion Beam by AC Glow Discharge
SUN Dian-ping, ZHU Yi, YANG Xiao-hua, YING Xu-ping, LIU Yu-yan, CHEN Yang-qin*
An experimental apparatus using an AC discharge was developed for producing molecular ion beam. The emission spectral intensity of N+2 is stronger than that of N2, and a very high ratio of N+2(B)/N2(C) up to 6∶1, was achieved in N2 discharge. The effects and processes of N2 discharge, and the concentration modulation spectra were studied. The vibrational temperature and rotational temperature of N+2 molecular ions beam were calculated to be 3 310 and 282 K respectively from the emission spectra.
2006 Vol. 26 (09): 1594-1597 [Abstract] ( 552 ) PDF (459 KB)  ( 399 )
1598 Study on Interaction of Anionic Surfactant SDS and Bovine Serum Albumin by Fourier Transform Infrared Spectroscopy
WANG Jing1, 2, GUO Chen1, LIANG Xiang-feng1, 2, ZHENG Li-li1, 2, CHEN Shu1, 2, MA Jun-he1, 2, LIU Hui-zhou1*
FTIR spectroscopy was applied to investigate the interaction of anionic surfactant Sodium Dodecyl Sulfate (SDS) and Bovine Serum Albumin (BSA). Amide band Ⅰ of BSA was analyzed to obtain the change in secondary structure of BSA when different concentration of SDS was added and during different interaction period. In short interaction period and at low concentration of SDS, the α-helixes increased and the random coil decreased. In long interaction period or at high concentration of SDS, SDS unfolded the protein by decreasing the α-helix structure and increasing the random coil.
2006 Vol. 26 (09): 1598-1600 [Abstract] ( 1197 ) PDF (324 KB)  ( 513 )
1601 Study on the Identification of Tea Using Near Infrared Reflectance Spectroscopy
ZHAO Jie-wen1, CHEN Quan-sheng1, ZHANG Hai-dong1, 2, LIU Mu-hua1, 3
A rapid tea identification method by near infrared spectroscopy coupled with pattern recognition based on principal components analysis and Mahalanobis’ distance technique was proposed. Four famous brand teas in China were studied, including Longjing tea, Biluochun tea, Maofeng tea and Tieguanyin tea in the experiment. In the spectral region between 6 500 and 5 300 cm-1, through preprocessing method of MSC(multiplicative scatter comection), the prediction model was built. The result showed that the model was the best with 8 principal component factors. The rates of identification in calibration set samples and prediction set samples were 98.75% and 95%, respectively. A new idea about quick and precise identification of tea was offered.
2006 Vol. 26 (09): 1601-1604 [Abstract] ( 1564 ) PDF (551 KB)  ( 483 )
1605 Application of NIR Quantitative Analysis to Nondestructive Detection of Loquat Soluble Solid Content
LI Jian-ping, FU Xia-ping, ZHOU Ying, YING Yi-bin*, XIE Li-juan, NIU Xiao-ying, YAN Zhan-ke, YU Hai-yan
The objective of the present research was to evaluate the potential of NIR diffuse reflectance spectroscopy as a way for nondestructive measurement of loquat soluble solid content of three varieties harvested from two orchards. According to the analysis, it was shown that the correlation coefficient of curves was relatively high in the two regions of 1 400-1 500 nm and 1 900-2 000 nm. Statistical models were developed using partial least square regression (PLSR), stepwise multiple linear regression (SMLR) and principal component regression (PCR) methods in the full spectral region from 800 to 2 500 nm and in the combined region of 1 400-1 500 nm and 1 900-2 000 nm. The results of PLSR model in the full spectral region were better than those of other models. The modeling results based on derivative spectra were not as good as those based on original spectra. Finally, a model was established based on the original spectra with 17 points smoothing for all the three varieties in the full spectral region by PLSR method. The correlation coefficients of calibration and validation were 0.96 and 0.95, respectively. The results indicate that it is feasible to use NIR spectroscopy technique for quantitative analysis of loquat soluble solid content.
2006 Vol. 26 (09): 1605-1609 [Abstract] ( 1654 ) PDF (601 KB)  ( 839 )
1610 Application of FTIR Spectroscopy to the Analysis of Quality Mensuration of Paeonia Lactiflora Pall. from Native Habitat
HONG Qing-hong1，2,CHENG Ze-feng2,CHENG Cun-gui2
In the present paper, different mensurations of Fourier transform infrared spectra for the analysis of Paeonia lactiflora Pall. were compared. FTIR mensurement was applied to the analysis of quality of xylem and outer layer of Paeonia lactiflora Pall. from native habitat. The results revealed that the chemical contents are the same in both the xylem and outer layer of Paeonia lactiflora Pall.. But outer layer has less paeoniflorin and its derivatives than in xylem of Paeonia lactiflora Pall. Different habitats of xylem of Paeonia lactiflora Pall.. were Hanzhou, Anhui and Sichuan. The results indicated that the quality with habitat in Zhejiang is the best among the three.
2006 Vol. 26 (09): 1610-1613 [Abstract] ( 1165 ) PDF (499 KB)  ( 446 )
1614 Calibration Transfer Used for the Interpretation of RS-FTIR Spectrum
ZHANG Lin, ZHANG Li-ming, LI Yan*, HU Lan-ping, WANG Jun-de
A technique of analysis for RS-FTIR (Remote Sensing Fourier Transform Infrared) spectrum was built based on the principle of calibration transfer. A system of four-component gas mixtures, whose FTIR spectra were overlapped seriously, was determined as the object in the present research. Its FTIR data from EPA and field measurement were used to set up calibration and prediction models, respectively. With OSC (orthogonal signal correction), the model built with data of EPA predicted values of field measurement. The RMSEP values of acetone, methanol, benzene and chloroform were 0.008 5, 0.018 0, 0.064 0 and 0.002 8, respectively, while without OSC correction the corresponding values were 0.085 6, 0.047 9, 1.065 3 and 0.014 2, respectively. With optimization, the Kennard-Stone method was used for subset selection, and the iteration in the process of OSC was carried out 3 times, yielding the best performance. The result indicates that this technique can overcome the limitation of background and calibration model, which restricts the application of RS-FTIR in air monitoring.
2006 Vol. 26 (09): 1614-1617 [Abstract] ( 1643 ) PDF (467 KB)  ( 426 )
1618 Application of Wavelength Selection Algorithm to Measure the Effective Component of Chinese Medicine Based on Near-Infrared Spectroscopy
GU Xiao-yu, XU Ke-xin, WANG Yan*
Near infrared (NIR) spectroscopy has raised a lot of interest in the pharmaceutical industry because it is a rapid and cost-effective analytical type of spectroscopy with no need for extensive sample preparation, and with the easy-realizable ability of on-line application. The NIR technology can increase the quality control standard of the Chinese medicine and accelerate the entry into the international market. In the present paper, two methods for wavelength selection are applied to the measurement of borneol, one of which is the multiple-chain stepwise, which tends to select many variables in the same area containing valuable information, and the other is the mixture genetic algorithm, which incorporates simulated annealing so as to improve the local searching ability while maintaining the global searching ability. The results present that the number of wavelength is reduced to 16% compared with the original number of wavelength, and the prediction accuracy has increased 47.6％. Therefore, the method of wavelength selection is a good way to enhance the prediction accuracy and simplify the model in NIR region.
2006 Vol. 26 (09): 1618-1620 [Abstract] ( 2206 ) PDF (428 KB)  ( 484 )
1621 A New Algorithm for Generating Raman Spectra Using Polychromatic Light Sources
XU Yi-zhuang1, WANG Jian-lin2, WU Jin-guang1
In the present paper, the possibility of generation of Raman spectra using polychromatic light instead of laser is discussed based on the theory of Raman scattering. When a polychromatic light impinges on a sample, the resultant scattering includes Rayleigh scattering and Raman scattering brought about by the excitation light at each corresponding frequency. Mathematical analysis demonstrates that the scattering spectra after being corrected by the frequency of scattered light are the convolution of Raman-Rayleigh jointed spectrum and the power spectrum of the excitation light. Therefore, Raman spectrum can be obtained with an algorithm of Fourier transformation using polychromatic light as an excitation source.
2006 Vol. 26 (09): 1621-1623 [Abstract] ( 1673 ) PDF (300 KB)  ( 406 )
1624 Fluorescence Spectrum of Cesium Vapor Resonantly Excited by the 852.3 nm Laser Line
LI Yuan-yuan，YIN Gui-qin，DAI Kang，SHEN Yi-fan*
The fluorescence spectrum of cesium vapor was studied in a cell irradiated with the light of 852.3 nm resonance line from a single mode laser diode. From the intensities and spectra of the Cs and Cs2 fluorescence we identified several collisional and radiative processes in the excited cesium atom-dimer system. The atomic lines of the highly excited states were the result of collisional energy transfer from Cs(6P3/2)+Cs(6P3/2)to Cs(6D，8S) atoms. The Cs2(B 1∏u) band formed in Cs(6P)+Cs2(X 1Σ+g) collisions. The fine-structure mixing in 6 2P atoms was studied through excitation transfer，energy-pooling collision, and collisional excitation transfer between Cs2 and Cs. The authors estimated the value of rate coefficient for the collisional energy transfer from 6P3/2 to 6P1/2 to be(5.2±2.1)×10-11 cm3·s-1. For the excitation transfer process Cs2(B 1∏u)+ Cs(6S)→Cs2(X 1Σ+g)+Cs(6P1/2)，a rate coefficient of(1.0±0.4)×10-9 cm3·s-1 was yielded.
2006 Vol. 26 (09): 1624-1626 [Abstract] ( 422 ) PDF (317 KB)  ( 462 )
1627 Study on the Inclusion Behavior of p-Sulphonatocalix[4]arene with Acridine by Spectrofluorometric Titrations
ZHOU Yun-you, LU Qin, LIU Chun, SHE Shi-ke, YANG Xu-lai, WANG Lun
p-sulphonatocalix[4]arene(1)was prepared according to the literature, and spectrofluorometric titrations were performed to investigate the inclusion behavior of (1) and acridine in citrate buffer solution (pH 5.92, 0.1 mol·L-1)at different temperatures. It was found that in definite concentration range, the emission peak of acridine exhibited a slight red shift and the fluorescence intensity decreased when (1) was added. They form stable host-guest complex, and the stoichiometry of the inclusion complex is 1∶1. The stability constants of the inclusion complex at 15.0 ℃, 20.0, 25.0 and 30.0 ℃ were determined as 3.08×105,4.45×104,2.58×104 and 8.90×103, respectively. The thermodynamic parameters of inclusion process, ΔG,ΔH and ΔS, were determined. The experimental results indicated that the inclusion process was an exothermic and enthalpy-driven process. It was found that the stability constants descended when temperature rose. The most probable pattern of the inclusion complex between (1) and acridine was proposed as: acridine partially goes into the cavity of (1), and the protonated N atom and the negatively charged sulphonyl group bond firmly owing to strong electrostatic interaction. With the main contribution of electrostatic interaction and the assistance of Van de Waals and hydrophobic interaction, the host and the guest molecules form 1∶1 supramolecular complex.
2006 Vol. 26 (09): 1627-1630 [Abstract] ( 1647 ) PDF (500 KB)  ( 446 )
1631 Study of the Fluorescent Response for Optical Ammonia Sensing Film
ZHANG Li-yan1, 2,ZHAO Li2,CHEN Xi2, 3*
The characters of optical sensing films for ammonia were investigated based on a fluorescent indicator aminofluorescein (AF) immobilization. An organically modified silicates (ORMOSILSs) obtained by copolymerizing various proportions of methyltrimethoxysilane (Me-TriMOS) and tetramethoxysilane (TMOS) was applied. Compared with the fluorescent responses of fluorescein and fluorescein carboxylicate, the fluorescent intensity enhancement of AF for ammonia was found to be caused by the reaction of ammonia with the NH2 group on AF. The reaction may cause the reduction of the intermolecular self-quenching of AF, resulting in a fluorescence enhancement of sensing film in NH3 solution. Furthermore，the properties and the ammonia-sensing behavior of the film were investigated. The results presented here emphasized the significance of ormosil as a matrix for dissolved ammonia sensing. The detection limit for ammonia in water was 0.3 μg·mL-1.
2006 Vol. 26 (09): 1631-1635 [Abstract] ( 1076 ) PDF (585 KB)  ( 443 )
1636 Study of Fluorescence Spectra of Starch Suspension
HE Hai-jian1,YU Rui-peng2,ZHU Tuo3*,GU Zheng-biao4,XU Hui1
To achieve detection, monitoring and automation of starch graft copolymerzation, the investigation was carried out according to the luminescence of free radical produced in the reaction. The investigation on the fluorescence spectra of starch-water suspension excited by ultraviolet light and its characteristics contributes to the study of the characteristics and mechanisms of free radical coming into being, emitting and disappearing. The experimental results indicate that starch-water suspension can emit fluorescence, whose peak wavelength is about 339 nm. Furthermore, the physical mechanism of fluorescence emission of starch is analyzed. It is considered that this fluorescence comes from the transition from nonbonding electrons in the hetero-atom (O) of the functional group (C—O—C) called ether linkage to the antibonding orbital σ*.
2006 Vol. 26 (09): 1636-1639 [Abstract] ( 1747 ) PDF (466 KB)  ( 423 )
1640 Fluorescence Investigation on Interaction between Artemisinin (Qinghaosu) and Hemin and Its Analytical Application
CHEN Li-hua1, 2, YIN Hong1, YANG Zhao-xia1, ZHANG Ke-mei1,LIU Liu-zhan2, SHEN Han-xi2
Hemin-catalytic decomposition of artemisinin (qinghaosu, QHS) was studied using pyronine B (PB) as an indicator. The interaction between hemin and QHS was an enzyme-substrate model, and the action sites were the endoperoxide moiety of QHS and the central metal ion of enzyme respectively. The kinetic catalytic constant depends upon enzyme and substrate concentrations, and the Michaelis-Menten parameters Km, Vmax and Kcat was 8.4×10-5 mol·L-1, 7.4×10-6 mol·L-1s-1 and 50.23 s-1 respectively. The catalytic activity of hemin was inhibited in the presence of deactivated agents and at high temperature. Under optimal conditions, the change in fluorescence intensity (F0-F) of pyronine B was proportional to the QHS concentration from 0.0 to 1.27×10-6 mol·L-1,and the detection limit (3σ) was as low as 2.3×10-8 mol·L-1. The proposed method was applied to detect the concentration of QHS in the media of plasma and urine.
2006 Vol. 26 (09): 1640-1643 [Abstract] ( 1147 ) PDF (495 KB)  ( 400 )
1644 Synthesis, Characterization and Fluorescent Properties of o, m, p-(Methacrylamido) Benzoic Acid
JIN Zheng-neng1,LU Jian-mei1, 2*,XU Qing-feng1,WANG Li-hua1
o, m, p-(Methacrylamido)benzoic acid (o, m, p-MAABA) was synthesized from o, m, p-aminobenzoic acid (o, m, p-ABA), elemental analysis and 1H NMR were used to confirm these compounds. The fluorescent properties of the compounds were investigated. The result showed that o, p-MAABA has λexmax at 358 nm while m-MAABA has λexmax at 324 nm. Excited at λexmax, o-MAABA had the strongest fluorescence, while p-MAABA had the weakest, and all of them were round at 420 nm.
2006 Vol. 26 (09): 1644-1648 [Abstract] ( 474 ) PDF (592 KB)  ( 440 )
1649 Characterization of Napropamide Enantiomers by CD and Determination of the Enantiomeric Ratios in Water
CHEN Sheng-wen1, CAI Xi-yun1, XI Meng1, ZHANG An-ping1, LIU Wei-ping1, 2*
The enantiomers of napropamide were separated by normal phase HPLC (HPLC: high performance liqaid Chroatography)with Chiralpak OJ-H column and charactered by circular dichroism. On this basis, a method for the chiral separation and micro-determination of napropamide in water was established. The linearity of calibration curve for racemic mixture was 10-100 ng·mL-1 and the correlation coefficient was 0.99. When 10 μL was injected，the detection limit of racemic mixture was 8 ng·mL-1, and the detection limits of both enantiomers were 4 ng·mL-1.
2006 Vol. 26 (09): 1649-1652 [Abstract] ( 1611 ) PDF (506 KB)  ( 410 )
1653 The Interaction of Acridine Orange with Anionic Surfactant and Its Application to the Determination of Protein
SHI Yan, ZHENG Wei-wan, WU Xiao-xing, ZHOU De-hong
The change in the UV-absorption spectrum and fluorescence spectrum of acridine orange(AO) due to the addition of surfactant dodecyi benzene sulfonic acid sodiumsait (SDBS) and bovine serum albumin(BSA) was studied. Meanwhile, the effects of the in situ formed AOAO dimer in SDBS as a fluorescence probe and BSA were discussed. A new method for the determination of BSA using fluorescence is presented. The results indicate that the method is sensitive and rapid. The linear range of determination is 0-4.17×10-7 mol·L-1. The relative standard deviation is 1.9%, and the detection limit is 8.73×10-10 mol·L-1.
2006 Vol. 26 (09): 1653-1655 [Abstract] ( 1682 ) PDF (372 KB)  ( 465 )
1656 Selective Determination for Calf Thymus DNA by a Resonance Light Scattering Technique with Morin-Ce(Ⅳ) System
ZHOU Min, MA Yong-jun, ZHANG De-yi, CHEN Hui*
The interaction of DNA and the binary complex formed between Ce(Ⅳ) and morin was studied. With the maximum scattering peak located at 320 nm, the enhanced RLS was proportional to the concentration of ctDNA in the range 0-25 μg·mL-1 and the detection limit (3σ) of 0.3 μg·mL-1 was obtained. Under the optimized conditions, four synthetic samples were determined with recoveries in the range 93.7%-108.4%. The proposed method is particularly attractive for its high selectivity for ctDNA in the presence of hsDNA by using certain sensitive spectrofluorometer since the intensity of RLS for hsDNA is far weaker than that for ctDNA.
2006 Vol. 26 (09): 1656-1659 [Abstract] ( 1125 ) PDF (424 KB)  ( 439 )
1660 Structure Changes of Lead Silicate Glasses Induced by UV Laser Irradiation
JIA Hong-zhi1,WANG Wen-cheng2
The structure changes of lead silicate glasses induced by UV laser irradiation were studied by UV-visible spectra and electron spin resonance (ESR). The Urbach energies of lead silicate glasses exposed to the 266 nm UV laser increased. This implied that the disorder of the lead silicate glasses was increased. The electron spin resonance experiments showed that the oxygen deficiency centers in lead silicate glass did not change to paramagnetic defects after exposure to 266 nm laser beam, and the absorption peak near 235 nm for lead silicate glass film did not change either. But this absorption peak was bleached after exposure to the 248 nm UV laser.
2006 Vol. 26 (09): 1660-1663 [Abstract] ( 2241 ) PDF (446 KB)  ( 397 )
1664 UV Spectroscopic Characterization of the Inclusion Interaction of β-Cyclodextrin and Sym-Diphenyl-Thiourea and Sym-Diphenyl-Urea
YAO Hong, WEI Tai-bao, XU Wei-xia, ZHANG You-ming*
The inclusion interaction of β-cyclodextrin and sym-diphenyl-thiourea and sym-diphenyl-urea was studied by UV spectra. The stoichiometry ratio for the formation of the inclusion complexes was determined by Hildebrand-Benesi equation linear analysis and molar ratio method. The standard molar Gibbs energies, enthalpies, and entropies were derived for the inclusion process by Ks at different temperatrues. The result showed that the host:guest ratio of inclusion complex between the two diphenyl compounds and β-CD is 2∶1, the stability constant(Ks) of 2∶1 inclusion complexes was higher than that of 1∶1 inclusion complexes due to cooperative binding in the close two hydrophobic cyclodextrin cavities, and the association of the guest molecule with β-CD was favored by enthalpy changes, proving that the Van der Waals interaction and the dipole-dipole interaction were main binding forces of cyclodextrin inclusion complex.
2006 Vol. 26 (09): 1664-1667 [Abstract] ( 2106 ) PDF (416 KB)  ( 448 )
1668 Studies on Aclacinomycin Spectral Characters and the Interaction between Aclacinomycin and DNA by Spectroscopic Method
HU Min1,2, ZHANG Zhen-xi1*,SHEN Guo-li3, LIU Ya-li3
The interaction of aclacinomycin(ACR) and DNA was investigated by fluorescence spectrum, and the characteristics of the fluorescence and absorption of aclacinomycin (ACR) were studied. The results indicate that there are two situations: in the case that the concentration ratio of ACR to DNA is small, the ACR is intercalated into the stacked base pairs of DNA; in the other case that the concentration ratio of ACR to DNA is great, the interaction between ACR and DNA is complex. The binding constant of the interaction between ACR and DNA, calculated by the fluorescence titration method, is 2.7 ×106 mol·L-1, and the binding site number is about 0.67 base pairs.
2006 Vol. 26 (09): 1668-1671 [Abstract] ( 1523 ) PDF (465 KB)  ( 450 )
1672 Interaction of Quercetin and Bovine Serum Albumin
WANG Chun, WU Qiu-hua, WANG Zhi*, CHEN Da-gang
The interaction of quercetin and bovine serum albumin (BSA) was investigated using fluorescence spectroscopy (FS) and ultraviolet spectroscopy (UV). The apparent binding constants (KA) between quercetin and BSA were 2.8×108(26 ℃) and 3.1×108(36 ℃), and the binding sites (n) were 1.7±0.02. According to the Frster theory of non-radiation energy transfer, the binding distances (r) were also obtained. The experimental results showed that the quercetin could be inserted into the BSA, quenching the inner fluorescence by forming the quercetin-BSA complex. It was found that both static quenching and non-radiation energy transfer were the main reasons for the fluorescence quenching. The process of binding was a spontaneous molecular interactio1n in which entropy increased while Gibbs free energy decreased, indicating that the interaction of quercetin and BSA was driven mainly by hydrophobic force.
2006 Vol. 26 (09): 1672-1675 [Abstract] ( 1676 ) PDF (475 KB)  ( 519 )
1676 Characteristics of Absorption Spectra of Phytoplankton
ZHANG Qian-qian1,WANG Lei2,LEI Shu-he3,ZHU Chen-jian1,WANG Xiu-lin1*
Nine typical phytoplankton species were chosen and cultivated under two temperatures (20 and 15 ℃) and two illuminations(7 000 and 1 100 lux), and their absorption spectra at different growth period were measured. Firstly, singular value decomposition was used on the matrix, which is composed of the autoscaled spectra data. The S1／(∑mi=1)Si was used to compare the similarities of spectra. Twenty-five representative spectra were obtained for the nine species of phytoplankton. Among them, there are one spectrum for each of Isochrysis galbana and Chaetoceros Debilis, two for each of Platymonas helgolanidica and Chaetoceros curvisetus, three for Gymnodinium sp., and four for each of Alexandrium tamarense, Prorocentrum dentatum, Skeletonema costatuma and Synechococcus sp. Then, feature extraction was processed to obtain the characteristic spectra, including seven incontinuous wavelengths that have great ability to differentiate species. They are 340.5-420.5 nm, 423.5-431 nm, 440.5-525.5 nm, 760.5, 763.5, 769.5 and 856.5 nm. Consequently, they form characteristic spectra. The discriminant result is 80%.
2006 Vol. 26 (09): 1676-1680 [Abstract] ( 2430 ) PDF (471 KB)  ( 550 )
1681 Study on the Structure and Optical Limiting Properties of Lead(Ⅱ) Tetrakis (4-Cumylphenoxy)-Phthalocyanine Doped Silica Gel Glass Composites
QIN Jing, LIN Zhi-peng, ZHAN Hong-bing*
The present paper reports the encapsulation of lead(Ⅱ) tetrakis (4-cumylphenoxy)-phthalocyanine (PbPc(CP)4) into silica gel glass matrix to produce homogeneously doped composites by sol-gel technique. The existing state and structure of the doped PbPc(CP)4 were studied by UV-Vis absorption spectra and TEM images. Optical limiting properties were measured at 532 nm with 8 ns pulses. The results show that the doped PbPc(CP)4 molecules aggregate to form clusters in the gel glass composites, and PbPc(CP)4 has better optical limiting properties in the gel glass composites owing to the rigid structure of solid matrix.
2006 Vol. 26 (09): 1681-1684 [Abstract] ( 597 ) PDF (552 KB)  ( 471 )
1685 Study on Interaction between Sulfonylurea Herbicides and Catalase by Fluorescence Spectroscopy
YE Fa-bing1, 2, DONG Yuan-yan1*, ZHOU Peng2, MO Xiao-man2, HU Xian-wen1
The binding of Sulfonylurea herbicides to catalase in aqueous solution was studied using fluorescence spectroscopy. It was shown that herbicides have a strong ability to quench the catalase fluorescence mainly through a static quenching procedure. The binding constant K and the number of binding site n were calculated according to the fluorescence quenching results. For chlorsufuron, K=8.69×105 L·mol-1 and n=1.16; for metsufuron methyl, K=1.01×106 L·mol-1 and n=1.21; and for bensufuron methyl, K=3.52×103 L·mol-1, n=0.77. It is clear that the binding of metsufuron methyl with catalase is stronger than that of chlorsufuron, while the binding of chlorsufuron stronger than that of bensufuron methyl.
2006 Vol. 26 (09): 1685-1687 [Abstract] ( 553 ) PDF (429 KB)  ( 399 )
1688 Determination of Antioxidation of the Extract from Chinese Medicine Morinda Officinalis How by Flow Injection Chemiluminescence and Spectroscopy
WU Yong-jun1, SHI Jie2, QU Ling-bo2, LI Fei-fei2, LI Xiao-jing2, WU Yi-ming1*
Flow injection chemiluminescence analysis was used to determine the antioxidation activity of extract from Morinda officinalis How. The determination was based on the inhibition effect of Morinda officinalis How extraction in the chemiluminescence reaction of luminol - H2O2-CuSO4 system, and vitamin C was used as positive control. It was showed that Morinda officinalis How original liquid has obvious anti-radical activity. Spectroscopy was applied to determine the inhibition ratio of Morinda officinalis How extraction for the superoxide anion and hydroxy radicals in the special system. It was shown that the extraction from Morinda officinalis How could obviously scavenge superoxide anion and hydroxy radicals. It is suggested that the Chinese Medicine Morinda Officinalis How is a potential antioxidation activity medicine.
2006 Vol. 26 (09): 1688-1691 [Abstract] ( 1129 ) PDF (471 KB)  ( 514 )
1692 New Approaches to Selenium Speciation
QIU Jian-hua, WANG Qiu-quan*, HUANG Ben-li
A review of the recent developments in selenium speciation analysis was presented, focusing on the techniques of separation, interface, elemental specific detection and identification of selenium species; the methods of sample storage and pretreatment storage for selenium speciation were also introduced.
2006 Vol. 26 (09): 1692-1701 [Abstract] ( 1138 ) PDF (1073 KB)  ( 453 )
1702 Determination of Trace Germanium by HG-ICP and Study on the Medium Influence
JIN Wei, ZHAO Cheng-yi*, MA Hui
A method to determine the content of trace germanium by hydride generation inductively coupled plasma atomic emission spectrometry was established. The influences of different acidic medium, concentration of NaBH4 and other concomitant elements are examined systemically. The optimal analytical conditions were discussed and examined. The detection limit is 0.2 ng·mL-1 and the precision is 0.9%. The value of germanium content detected in environmental reference material agrees with the certified value. The medium influence on determining trace germanium was studied in detail.
2006 Vol. 26 (09): 1702-1706 [Abstract] ( 1164 ) PDF (530 KB)  ( 467 )
1707 Effect of Nebulizer Gas Pressure on the Emission Intensity of ICP
CHEN Jin-zhong, HA Jing, WEI Yan-hong, GUO Qing-lin, HUAI Su-fang
In an attempt to inhance the emission intensity of ICP, a conventional concentric pneumatic nebulizer was used with increased pressure, and the aqueous aerosol was partially let out to modulate the flow velocity of sample solution into the flame of the ICP. In the present work, the emission spectra of elements Ca, Si, Sr and Zn in water samples were measured, and the results showed that corresponding emission intensities are the highest at 0.05 MPa under the conventional condition, and after increasing the nebulizer gas pressure to 0.07 MPa and letting out part of aqueous aerosol, the peak values grew by 38%, 79%, 45% and 70%, respectively. In addition, the stability of the plasma radiation was not affected by the higher nebulizer gas pressure.
2006 Vol. 26 (09): 1707-1709 [Abstract] ( 1675 ) PDF (395 KB)  ( 471 )
1710 Determination of Elements Related to Reducing Blood Sugar(ERBS)in Tea and Tea Polysaccharide by ICP-MS
XIE Ming-yong1, NIE Shao-ping1, FU Bo-qiang2, WANG Xiao-ru2
Contents of elements related to reducing blood sugar (ERBS) in several kinds of green tea of Wuyuan county, their tea infusion extracted by warm water and boiling water and tea polysaccharide (TP) were determined by ICP-MS, after the samples were digested with acids by microwave method. The relation between the amount of ERBS and tea quality and the relation between the content of ERBS in TP and the total content in tea were discussed. The results showed that the amounts of ERBS in tea and TP are different in various samples. The amount of ERBS in TP extracted from coarse tea was higher than that from fresh tea. The percentage of certain ERBS in TP accounting for the ERBS in tea ranged from 0.03% to 9.57%. As to the total ERBS in TP extracted from Mt. Zhang super grade tea and grade 5 tea, the percentages were 1.11% and 2.10% respectively. As to Mt. Gu old tea, it was 0.85%. The results could provide data for selecting tea material used to extracting tea polysaccharide and for studying the mechanism of reducing blood sugar.
2006 Vol. 26 (09): 1710-1715 [Abstract] ( 379 ) PDF (626 KB)  ( 441 )
1716 Analysis of the Degrading Products of Carbofuran by Sphingomonas sp. with GC/MS and GC/FTIR
WU Jun1,HONG Qing1, 2,CHEN Yi-nan1,LI Shun-peng1*
In different degrading phases，the degrading products of carbofuran by CDS-1(Sphingomonas sp.) were extracted by acetone. According to the analysis of gas chromatography/mass spectrometry (GC/MS)，carbofuran-7-phenol was identified as the initial degrading product, and an unknown metabolite with molecular weight of 182 was determined as 2-hydroxyl-3-tertiarybutylalcohol-phenol, which was the next degrading product of carbofuran-7-phenol. According to the analysis of GC/MS and gas chromatography/Fourier transform infrared spectrum (GC/FTIR)，the main component of the compounds that appeared in the final phase of degradation which could produce volatile pungent odor，was identified as carvone.
2006 Vol. 26 (09): 1716-1719 [Abstract] ( 1808 ) PDF (499 KB)  ( 426 )
1720 Determination of 27 Microelements in 11 Shellfishes
HE Jin-zhe, ZHAO Pei-cheng, YANG Kai, ZHANG Jian-you, SUN Pei-long
Shellfish samples were prepared by microwave, and 27 microelements including Ca, Mg, K, Na, P, S, Zn, Mn, Fe, Cu, Cr, Co, Se, Sn, Ni, Al, Ti, V, Mo, Ba, Ge, Si, Cd, I, Pb, As and Hg were determined by ICP-AES. It was showed that ICP-AES had an obvious advantage in the determination of microelements, and shellfishes contained upwards of 20 kinds of microelements. Calcium in fresh water was higher than that in seawater, and As and Pb were the major pollutants.
2006 Vol. 26 (09): 1720-1723 [Abstract] ( 507 ) PDF (403 KB)  ( 410 )
1724 Atomic Emission Spectrometry Determination of Au, Pt and Pd after Separation and Enrichment by Hyperbranched Polymer
LI Hui-zhi1, ZHAI Dian-tang1, SHOU Chong-qi2, ZHAO Shu-ying1, WEI Qin1
The present paper shows that the trace amount of gold, platinum and palladium in hydrochloric acid solution can be concentrated by hyperbranched polymer. The new reagent has a rapid adsorption rate and big concentrating capacity. The determination of trace Au, Pt and Pd in sample using carbon powder and strontium carbonate as buffer was carried out by atomic emission spectrometry(AES). Zirconium was selected as internal standard line. The sample was directly loaded into ordinary electrode. The method is simple, rapid and accurate. The condition of determination, and factors of influence were studied. The analysis line of Au, Pt and Pd is 312.3, 306.5 and 311.4 nm respectively. The internal standard line of Zr is 310.7 nm. The linear range of the determination of Au, Pt and Pd is 0-0.20%, 0-0.40% and 0-0.20% respectively. The detection limit of Au, Pt and Pd is 0.010%, 0.003 0% and 0.003 0% respectively. The method has been applied to the determination of Au, Pt and Pd with satisfactory results.
2006 Vol. 26 (09): 1724-1727 [Abstract] ( 610 ) PDF (508 KB)  ( 445 )
1728 Determination of 15 Trace Elements in Antineoplastic Traditional Chinese Medicine by Atomic Absorption Spectrometry
LIU Yan-ming, WANG Hui, HAN Jin-tu, LOU Zhi-ge, YUE Shi-ping
Five kinds of antineoplastic traditional Chinese medicines including fufangtianxian capsule, xiaoaiping tablet, zhiling capsule, shenlian capsule and shenlinglan capsule were digested with HNO3-HCl mixed acid. The fifteen trace elements including calcium, magnesium, potassium, sodium, iron, zinc, manganese, cobalt, nickel, chromium, silver, copper, strontium, cadmium and lead in the above-mentioned drugs were determined by atomic adsorption spectrometry. The effects of the kinds of mixed acid, the ratio of HNO3 to HCl in the mixed acid, the volume of digesting solution and the digesting time were also investigated in detail. The results obtained show that the concentrations of Ca, Mg, K, Na, Fe, Zn, Mn, Co and Ni in the five kinds of antineoplastic Chinese traditional medicines are higher than those of other elements.
2006 Vol. 26 (09): 1728-1731 [Abstract] ( 215 ) PDF (508 KB)  ( 443 )
1732 Determination of Trace Lead and Iron in Nickel Chloride and Manganese Sulfate by Flame Atomic Absorption Spectrometry after Coprecipitation with Yttrium Phosphate
SU Yao-dong, ZHU Wen-ying, MA Hong-mei, CHEN Long-wu
Using yttrium phosphate as the coprecipitation collector for the separation and preconcentration of trace lead and iron in nickel chloride and manganese sulfate, flame atomic absorption spectrometric (FAAS) determination was described in the present paper. Coprecipitation parameters including the pH of the solution, and the amounts of YCl3 and H3PO4 were discussed. It was found that lead and iron in nickel chloride could be coprecipitated quantitatively in the range of pH 3.0～4.0, and so could be lead in manganese sulfate. The detection limits (3σ) of lead and iron in 20 mL solution were 1.63×10-2 mg·L-1 and 4.58×10-2 mg·L-1 respectively. In NiCl2 solution the standard addition recoveries for lead and iron were 100.91% and 99.73% respectively, and in MnSO4 solution the standard addition recoveries were 99.45% and 98.98% respectively. The method has eliminated the interference of matrix, and the result is satisfied.
2006 Vol. 26 (09): 1732-1734 [Abstract] ( 1109 ) PDF (394 KB)  ( 453 )
1735 Determination of Trace Elements in Spirulina Platensis (Notdst.) Geitl. by Flame Atomic Absorption Spectrometry Combined with Microsampling Pulse Nebulization Technique
CHENG Cun-gui1,HONG Qing-hong1，2,LI Dan-ting1,FAN Meng-hai1,CAI Xiao-dan1
The contents of trace elements Ni，Zn，Mn，Cu，Mg，Fe，Ca and Pb in Spirulina platensis (Notdst.) Geitl. were determined by flame atomic absorption spectrometry combined with microsampling pulse nebulization technique. The results of the determination show that Spirulina platensis (Notdst.) Geitl. are rich in the inorganic elements such as Mg, Zn, Fe, Ca and Cu. Its recovery ratio obtained by standard addition method ranged between 96.58% and 106.12%, and its RSD was lower than 4.26%. The result will provide scientific data for the study on the trace elements in Spirulina platensis (Notdst.) Geitl. and on their relativity of efficacy of medicine.
2006 Vol. 26 (09): 1735-1737 [Abstract] ( 2092 ) PDF (437 KB)  ( 469 )
1738 A Method for Obtaining Redshifts of Quasars Based on Wavelet Multi-Scaling Feature Matching
LIU Zhong-tian1, LI Xiang-ru1, WU Fu-chao1, ZHAO Yong-heng2
The LAMOST project, the world’s largest sky survey project being implemented in China, is expected to obtain 105 quasar spectra. The main objective of the present article is to explore methods that can be used to estimate the redshifts of quasar spectra from LAMOST. Firstly, the features of the broad emission lines are extracted from the quasar spectra to overcome the disadvantage of low signal-to-noise ratio. Then the redshifts of quasar spectra can be estimated by using the multi-scaling feature matching. The experiment with the 15，715 quasars from the SDSS DR2 shows that the correct rate of redshift estimated by the method is 95.13% within an error range of 0.02. This method was designed to obtain the redshifts of quasar spectra with relative flux and a low signal-to-noise ratio, which is applicable to the LAMOST data and helps to study quasars and the large-scale structure of the universe etc.
2006 Vol. 26 (09): 1738-1741 [Abstract] ( 1177 ) PDF (407 KB)  ( 448 )
1742 Transient Dynamics of Excited States and Nonlinear Optical Properties of InP Nanoparticles
LI Dan1,YANG He-qing2,LIANG Chun-jun1,QIAN Shi-xiong3
The decay curves of the emission at different wavelength were measured. One nanosecond of transition lifetime of interband was obtained. The transient dynamics of InP nanoparticles was investigated by one color femtosecond pump-probe method at the wavelength of 800 nm. The experimental result shows that the saturation of exciton resonance results in photo-bleaching at 800 nm The decay process of the bleaching includes two components, i.e. the fast one from free carries scattering and the slow one from trapped carriers scattering. The nonlinear optical properties of InP nanoparticles were investigated by using femtosecond optical Kerr effect. The magnitude of χ3 for InP nanoparticles embedded in SiO2 sol-gel glass was calculated, and its origin was analyzed.
2006 Vol. 26 (09): 1742-1745 [Abstract] ( 1160 ) PDF (438 KB)  ( 427 )
1746 The Research on Preparing CeO2 Nanocrystalline by Homogeneous Precipitation Method
Garidi1, LI Xia1*, LI Li2, Zhaorigetu1
CeO2 nanocrystallines were prepared by homogeneous complexed-precipitation method, using cerous nitrate and ammonium tartrate as raw materials. The effects of cerous tartrate complex compound and the way of producing precipitation on the particle size of samples were investigated. The samples were characterized by XRD, TEM and SEM. The SEM micrograph shows that the foam exhibits a perforated porousness stereostructure in shape, and the HRTEM picture of the particles reveals the clear crystal lattice. All the results indicate that the samples were CeO2 nanocrystalline.
2006 Vol. 26 (09): 1746-1748 [Abstract] ( 993 ) PDF (752 KB)  ( 452 )
1749 Nitrogen Stress Measurement of Canola Based on Multi-Spectral Charged Coupled Device Imaging Sensor
FENG Lei1, FANG Hui1, ZHOU Wei-jun2, HUANG Min1, HE Yong1*
Site-specific variable nitrogen application is one of the major precision crop production management operations. Obtaining sufficient crop nitrogen stress information is essential for achieving effective site-specific nitrogen applications. The present paper describes the development of a multi-spectral nitrogen deficiency sensor, which uses three channels (green, red, near-infrared) of crop images to determine the nitrogen level of canola. This sensor assesses the nitrogen stress by means of estimated SPAD value of the canola based on canola canopy reflectance sensed using three channels (green, red, near-infrared) of the multi-spectral camera. The core of this investigation is the calibration methods between the multi-spectral references and the nitrogen levels in crops measured using a SPAD 502 chlorophyll meter. Based on the results obtained from this study, it can be concluded that a multi-spectral CCD camera can provide sufficient information to perform reasonable SPAD values estimation during field operations.
2006 Vol. 26 (09): 1749-1752 [Abstract] ( 394 ) PDF (566 KB)  ( 515 )
1753 Simultaneous Determination of Total Flavone and Total Saponin in Gynostemma Pentaphyllum by Signal Multiplier Spectrophotometry
HUI Rui-hua, HOU Dong-yan, LI Tie-chun, LIU Xiao-yuan
Important chemical constituents analysis for the total flavone and total saponin in gynostemma pentaphyllum is described. The colour reactions of flavones and saponines with vanillin-perchloric acid in acetic acid produced the good absorptions at 451 and 547 nm, but the absorption peaks too overlapped to be determined simultaneously. A new method for the total flavone and the total saponin in gynostemma pentaphyllum to be determined by signal multiplier spectrophotometry simultaneously without any preliminary separation was proposed. For quantitative analysis, the rutinum as a standard of the total flavone and the ginsenoside Rb1 as standard of the total saponin were applied. The experiment results showed that the regression equations of concentration and ΔA were obtained：ΔAflavone=0.013 3+4.417 0cflavone,relation coefficient rflavone=0.999 4, and the total flavone concentrations were in 0-0.16 μg·mL-1 with ΔA obeying linear relation; ΔAsaponin=2.775 5csaponin-0.888 1×10-2,relation coefficient rsaponin=0.999 1, and the total saponin concentrations were in 0-0.30 μg·mL-1 with ΔA obeying linear relation respectively. The recovery ratio was 104.0%-113.0 % and 86.8%-94.6% respectively. The RSDflavone was less than 0.58%(n=9) and RSDsaponin was less than 0.35%(n=9) respectively. The proposed method is simple, rapid accurate and feasible.
2006 Vol. 26 (09): 1753-1756 [Abstract] ( 598 ) PDF (432 KB)  ( 449 )
1757 Comparison of Light Attenuation Characteristics and Optical Penetration Depths between Native and Coagulated Human Liver Tissues
WEI Hua-jiang1, GUO Zhou-yi1*,XIE Shu-sen2,GU Huai-min1,WU Guo-yong3,HE Bo-hua4,JIN Ying1
A double-integrating-spheres and IAD method were used to study the differences in the optical penetration depths (OPDs) and light attenuation (LA) native and coagulated human liver tumors and liver tissues at the wavelengths of 680, 720, 780, 810, 850 and 890 nm of Ti:Sapphire laser. The results of measurement showed that the OPDs for native and coagulated human liver tumors and liver tissues at six different wavelengths obviously increase with increasing laser wavelength, the OPDs of coagulated human liver tumors and liver tissues at six different wavelengths were significantly smaller than that of native human liver tumors and liver tissues at the same wavelength respectively (P＜0.05), and the OPDs of native and coagulated human liver tumors at six different wavelengths were significantly bigger than that of native and coagulated human liver tissues at the same wavelength respectively (P＜0.05). The LA for native and coagulated human liver tumors and liver tissues at six different wavelengths obviously decreases with increasing laser wavelength, and the LA for coagulated human liver tumors and liver tissues at six different wavelengths is significantly bigger than that for native human liver tumors and liver tissues at the same wavelength respectively (P＜0.05). The LA for native and coagulated human liver tumors at six different wavelengths is significantly bigger than that for native and coagulated human liver tissue at the same wavelength respectively (P＜0.05).
2006 Vol. 26 (09): 1757-1760 [Abstract] ( 1802 ) PDF (446 KB)  ( 433 )
1761 The Real-Time FOCSDT Detection Method and Conditions for Dissolution Test of Metronidazole and Vitamin B6 Tablets
WU Jun1, YANG Mei2, LI Xin-xia3, CHEN Jian3
A method to monitor the dissolution rate of drug on-line fiber optic chemical sensor dissolution test(FOCSDT) was studied. Bifurcated optical fiber was used to connect the light source and detector, and the common end was dipped in the dissolution vessel. The dissolution process could be monitored through computer. The results show that the high, middle and low concentration group’s recovery of metronidazole is 100.8%, 99.8% and 100.6%; and RSD is 2.5, 0.8 and 1.1 respectively, The high, middle and low Concentration group’s recovery of vitamin B6 is 98.8%, 100.8% and 98.8%; and RSD is 4.1, 4.1 and 2.5 respectively. This process of analysis can test the whole process of drug and get dissolution graph. The study shows that this process analysis can reflect the real dissolution of drug and obtain the total information.
2006 Vol. 26 (09): 1761-1764 [Abstract] ( 1623 ) PDF (497 KB)  ( 855 )
1765 Determination of Writing Age of Blue Ballpoint Pen Inks by High Performance Liquid Chromatography
SHI Xiao-fan1, LI Xin-qian1, XU Ying-jian1, WANG Jing-han1, WANG Yan-ji2
As it is a frequently encountered problem in the laboratory of forensic science nowadays to distinguish whether the questioned documents, such as deeds, contracts, and receipts, written in ballpoint pen inks are true or not, and identify the writing age of them, it is very essential to establish a simple, sensitive and accurate method to examine the similarities and differences of the ballpoint pen inks and identify the writing age. The present paper introduces a technique that allows identifying the kind and the writing age of the blue ballpoint pen inks. The technique is based on using a high performance liquid chromatographic method for distinguishing the similarities and differences in dyes of blue ballpoint pen inks and determining changes in dyes of blue ballpoint pen inks developed with age, and these changes can be evaluated by the ratio of peak areas.
2006 Vol. 26 (09): 1765-1768 [Abstract] ( 2510 ) PDF (530 KB)  ( 499 )
1769 Microbiochemical Analyzer Based on Continuous Spectrum and Its Test for Clinic Use
HU Song, WEN Zhi-yu, LIANG Yu-qian, DU Xiao-qing, ZHANG Bo
A microbiochemical analyzer based on continuous spectrum and its test for clinic use are introduced. The principle of splitting behind was adopted to design the micro biochemical analyzer, which has the characteristics of little disorder light, real time collection of continuous spectrum signal(340-770 nm), small volume, light weight, small wastage of sample and reagent and rapidness and direct view of check. It has wide application in the emergency treatment, middling and small hospitals, rescue of battleground, and medicinal research. Representative items for clinic test were selected, such as uric acid, total cholesterin and albumin in our experiments. Comparative test and analysis of the daily check-up samples were made respectively by our microbiochemical analyzer and by the standard one of the hospital(Olympus AU2700, RT-1904C, Beckman LX-20). The test results show that our micro biochemical analyzer can meet the requirement of clinic use.
2006 Vol. 26 (09): 1769-1773 [Abstract] ( 2227 ) PDF (565 KB)  ( 409 )

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