TLC原位拉曼光谱法快速检测减肥类保健食品中非法添加的四种成分

doi:10.3964/j.issn.1000-0593(2018)03-0830-07

Abstract
References
Related Citation (15)

Download: PDF (4751 KB)
Export: BibTeX | EndNote (RIS)

Abstract To establish a thin layer chromatography (TLC) combined with Raman spectroscopy method and detect four chemical components (theophylline, caffeine, nandrolone phenylpropionate, spironolactone) added illegally in the slimming health food. The doping ingredient and substrate were separated with TLC, viewed and located with UV light (254 nm), the spot of the doping ingredient is concentrated on the TLC plate before detecting the Raman spectroscopy of the concentrated point directly with 780 nm laser source. The effects of different formulations of slimming health food were studied, the limit of detect (LOD) was validated, and the TLC Situ Raman Spectroscopy method was established initially for detecting four chemical components added in the slimming health food. The common substrate in the slimming health food had no interference with the detecting results of doping components. The LOD was 2~4 μg. Two out of nine kinds of health food were detected to be with caffeine. In this study, we established TLC and Raman spectroscopy for the first time which was used for quick identification of four kinds of illegal chemicals added in health food, such as theophylline, etc. The method is precise, sensitive, rapid and simple, which could provide a new reference for the technology research of rapid detection of the ingredients added illegally in health food.

Key words：TLC; Raman spectroscopy in situ; Concentration in situ; Slimming health food; Added illegally

Received: 2016-10-27 Accepted: 2017-03-05

ZTFLH:

O657.3

Corresponding Authors: LI Li E-mail: lilianlinsuo@163.com

Cite this article:

URL:

https://www.gpxygpfx.com/EN/10.3964/j.issn.1000-0593(2018)03-0830-07 OR https://www.gpxygpfx.com/EN/Y2018/V38/I03/830

[1] GE Bao-kun, ZHANG Qun-qian, ZHAO Kong-xiang(葛宝坤, 张群谦, 赵孔祥). Chinese J. Pharm. Anal.(药物分析杂志), 2012, 32(11): 2034.
[2] WANG Jing-wen, HUANG Xiang-lu, CAO Jin, et al(王静文, 黄湘鹭, 曹进, 等). J. Chromatogr.(色谱), 2014, 32(2): 151.
[3] HUANG Yi-bin, HUANG Nuo-jia, YANG Wen-hong(黄奕滨, 黄诺嘉, 杨文红). Qilu Pharm Affairs(齐鲁药事), 2011, 30(1): 12.
[4] ZHANG Xiao-dan, FU Xiao-ying, CHEN Jing, et al(张晓丹, 傅小英, 陈静, 等). Chinese J. New Drugs.(中国新药杂志), 2012, 21(2): 195.
[5] GAN Yong-qiang, JI Nan, TIAN Ping, et al(甘勇强, 纪南, 田萍, 等). China Pharmacist(中国药师), 2014, 17(10): 1675.
[6] XU Feng, LI Li, ZHANG Hong-lian, et al(许凤, 李莉, 张宏莲, 等). Chinese J. Pharm. Anal.(药物分析杂志), 2014, 34(9): 1634.
[7] HUANG Nuo-jia, YANG Wen-hong, HUANG Yi-bin(黄诺嘉, 杨文红, 黄奕滨). Food Drug(食品与药品), 2011, 13(3): 114.
[8] ZENG Tao(曾涛). China Pharmacist(中国药师), 2007, 10(6): 599.
[9] ZHU Jian, QIU Yi-jing, SHEN Guo-fang(朱健, 裘一婧, 沈国芳). Chinese Traditional Herbal Drugs(中草药), 2014, 45(4): 509.
[10] Lv D Y, Cao Y, Lou Z Y, et al. Anal. Bioanal. Chem., 2015, 407: 1313.
[11] QIU Ying-huan, WANG Tie-jie, LI Yu-lan, et al(邱颖姮, 王铁杰, 李玉兰, 等). Chinese J. Pharm. Anal.(药物分析杂志), 2008, 28(2): 323.
[12] LI Zhi-cheng, LIU Xiao-ling, GAN Sheng, et al(李志成, 刘小玲, 甘盛, 等). Sci. Technol. Food Industry(食品工业科技), 2015: 36(3), 324.
[13] XU Feng, YANG Ying, LI Li, et al(许凤, 杨莹, 李莉, 等). J. Hyg. Res.(卫生研究), 2013, 42(2): 302.
[14] ZHU Zi-ying(朱自莹). Applications of Raman Spectroscopy in Chemistry(拉曼光谱在化学中的应用). Shenyang: Northeastern University Press(沈阳: 东北大学出版社), 1998.
[15] LIU Yuan-rui, GE Hai-sheng, ZHAO Kang-hu, et al(刘元瑞, 葛海生, 赵康虎, 等). Chinese J. Pharm. Anal.(药物分析杂志), 2014: 34(7): 1241.
[16] WANG Yu, CAO Ling, LUO Jiang-nan(王玉, 曹玲, 罗疆南). Chinese Pharm. J.(中国药学杂志), 2011, 46(10): 789.
[17] WANG Dong-mei, LIU Yu-lu, MENG Qing-nan, et al(王冬梅, 刘玉路, 孟庆楠, 等). J. Qiqihar University Med.(齐齐哈尔医学院学报), 2007, 28(1): 68.
[18] HE Yu, HONG Xiao-kun, WANG Zhi-hua(何昱, 洪筱坤, 王智华). Chinese Traditional Patent. Med.(中成药), 2003, 25(10): 827.