|
|
|
|
|
|
Spectral Study on the Interaction between Cytochrome c and ProliNONOate |
TANG Qian1, 2, GONG Ting-ting1, 2, SHI Shan-shan1, 2, CAO Hong-yu1, 2, WANG Li-hao3, YU Yong3, SHAN Ya-ming3, ZHENG Xue-fang1, 2* |
1. School of Life Science and Biotechnology, Dalian University, Dalian 116622, China
2. Liaoning Key Lab of Bio-organic Chemistry, Dalian 116622, China
3. College of Life Sciences, Jilin University, Changchun 130012, China |
|
|
Abstract It played an important role in the detection of mitochondrial apoptosis that the interaction of Cytochrome(Cyt c) with NO, thus the research is still a hotspot issue for the chemists and biologists. In this paper, Ultraviolet (UV-Vis) absorbance spectra, UV-Vis time course spectra, circular dichroism (CD), synchronous fluorescence spectra and electron paramagnetic resonance (EPR) technology were used to research the coordination reaction mechanism of different valence states Cyt c with proliNO/NO and its spatial conformation changes. The results showed that Cyt c could directly react with NO without other reagents. And its secondary structure would change during the experiment. It has been established that the electronic configurations of iron ions in porphyrin complexes are controlled in the nature by a number of axial ligands, peripheral substituents attached to the porphyrin macrocycle, deformation of the porphyrin ring and solvent effects. During the experiment, proliNO/NO was added to the sample of Cyt c, and NO gas would generate, then entered into the solution, finally the distal methionine heme ligand was displaced, Cyt c could bind NO, that is, the Fe—S broke down, and the Fe-N formed. At the same time, generating a new cytochrome c-NO (Cyt c-NO) complex. Cyt c-NO binary complexes were instability and would dissociate, and dissociation process of Cyt c-NO binary complexes was belonging to a first-order reaction with the dissociation rate of (0.071 1±0.039 6) s-1. The secondary structure of Cyt c was affected by proliNO/NO concentration. When the concentrate of proliNO/NO was below to 8.6×10-4 mol·L-1, the peak changes of 222 nm and 208 nm was very weak, and the α-helix increased from 33.1% to 44.1%. And it continued to increase, the secondary structure of Cyt c took place a great change. The tiny changes illustrated that a new compound generated, but excessive proliNO/NO can break the structure of Cyt c. Taken together, we have demonstrated that an understanding of the interaction mechanism of NO with cytc, and the reaction mechanism of NO with ferri- and ferro-cytochrome c have important implications for the inhibition of mitochondrial oxygen consumption by NO and the mitochondrial metabolism of NO.
|
Received: 2015-04-15
Accepted: 2015-10-19
|
|
Corresponding Authors:
ZHENG Xue-fang
E-mail: dlxfzheng@126.com
|
|
[1] Nienhaus K, Palladino P, Nienhaus G U. Biochemistry, 2007, 47(3): 935.
[2] MA Jing, ZHENG Xue-fang, TANG Qian,et al(马 静,郑学仿,唐 乾,等). Chemical Journal of Chinese University(高等学校化学学报), 2008, 29(2): 1.
[3] Larson S K, Dwyer D S, Lo H H,et al. Biochemical and Biophysical Research Communications, 2006, 342(3): 991.
[4] WANG Yan-jie, DENG Wen, ZHANG Peng-fei(王艳杰, 邓 雯, 张鹏飞). Progress in Veterinary Medicine(动物医学进展), 2012, (7): 89.
[5] Basu S, Azarova N A, Font M D,et al. Journal of Biological Chemistry, 2008, 283(47): 32590.
[6] XU Jian-xing(徐建兴). Bulletin of Chinese Academy of Sciences(中国科学院院刊), 2003, (4): 277.
[7] Cooper C E, Mason M G, Nicholls P. Biochimica et Biophysica Acta, 2008, 1777(7-8): 867.
[8] ZHOU Hua-wei, CAO Hong-yu, TANG Qian,et al(周华伟, 曹洪玉, 唐 乾, 等). Chin. J. Inorg. Chem.(无机化学学报), 2011, 27(3): 445.
[9] TANG Qian, ZHANG Yue, CAO Hong-yu,et al(唐 乾, 张 越, 曹洪玉, 等). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2015, 35(7): 1967.
[10] ZHANG Guo-hua(章国华). Chemistry Teaching And Leaning(化学教与学), 2011, (3): 78.
[11] QIU Xiang-jun, DING Bao-jin(邱相君, 丁保金). Chinese Journal of Clinical Pharmacology and Therapeutics(中国临床药理学与治疗学), 2003, (1): 118.
[12] CAI Zhen, SHI Li-fu(蔡 溱, 石力夫). Foreign Medical Sciences·Section of Pharmarcy(国外医学·药学分册), 2001, (4): 237.
[13] Orii Y, Shimada H. Journal of Biochemistry, 1978, 84(6): 1543.
[14] Zhang Pingyu, Wang Jingquan, Huang Huayi, et al. Biomaterials, 2014, 35: 9003.
[15] Sharpe M A, Cooper C E. Biochem. J., 1998, 332(1): 9.
[16] Silkstone R S, Mason M G, Nicholls P,et al. Free Radical Biology and Medicine, 2012, 52(1): 80.
[17] Grubina R, Basu S, Tiso M,et al. Journal of Biological Chemistry, 2008, 283(6): 3628.
[18] Butt W D, Keilin D. Proc. R Soc. Lond. B Biol. Sci., 1962, 156: 429.
[19] Zhao X J, Sampath V, Caughey W S. Biochemical and Biophysical Research Communications, 1994, 204(2): 537.
[20] Jayakumari N R, Reghuvaran A C, Rajendran R S,et al. Journal of the Nitric Oxide Society, 2014, 43: 35.
[21] CHEN Guo-zhen, HUANG Xian-zhi, ZHENG Zhu-zi, et al(陈国珍, 黄贤智, 郑朱梓, 等). Fluorescence Analysis(荧光分析法). Beijing: Science Press(北京: 科学出版社), 1990.
[22] Jayakumari N R, Reghuvaran A C, Rajendran R S et al. Biology and Chemistry/Official Journal of the Nitric Oxide Society, 2014, 43: 35. |
[1] |
CHENG Hong1, YAN Ding-ce1*, WU Li-qing2, XU Jun3. Spectral Magnitude Uncertainty in Measurement of Protein Circular
Dichroism Spectra—An Empirical Study on Cytochrome C[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(10): 3105-3110. |
[2] |
AN Huan1, YAN Hao-kui2, XIANG Mei1*, Bumaliya Abulimiti1*, ZHENG Jing-yan1. Spectral and Dissociation Characteristics of p-Dibromobenzene Based on External Electric Field[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(02): 405-411. |
[3] |
ZHANG Gui-xiong, YANG Xiao-xia*, LIU Kai, FU Feng*. Effect of Sequential Solvent Treatment on the Macromolecular Structure of Organic Matter in Zichang Coal[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2021, 41(09): 2934-2939. |
[4] |
ZHANG Kai-lin1, 2, ZHOU Min3, SHI Ying-ying2, LI Shu-qi2, MA Li-fu1, ZHANG Xian-yi3*, WANG Yan1, KONG Xiang-lei2, 4*. Development and Application of an Automated Program for Photodissociation Spectroscopy Study Based on a FT ICR Mass Spectrometer[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2021, 41(08): 2325-2331. |
[5] |
SONG Fan-hao1, 2, LI Ting-ting2, ZHANG Jin3, LIU Sha-sha2, FENG Wei-ying2, HE Jia2, BAI Ying-chen2*. Proton Binding Heterogeneity in Soil Fulvic Acid Sub-Fractions Using Two-Dimensional Correlation Fluorescence Spectrometry[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2019, 39(10): 3071-3077. |
[6] |
TANG Qian1, 2, ZHANG Yue1, CAO Hong-yu1, 2, SHI Shan-shan2, ZHENG Xue-fang1, 2* . Spectral Study on Coordination Reaction between metMyoglobin and Nitric Oxide[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2015, 35(07): 1967-1972. |
[7] |
DENG Chun-feng, LU Biao, WU Chun-lei, WANG Yi-fu, WEN Zhong-wei. Two-Temperature Diagnostic Studies by Emission Spectra for Nonequilibrium Ti-H Plasma[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2014, 34(12): 3374-3379. |
[8] |
ZHU Yong-le, WANG Shu-ying, LIU Jing, ZHONG Chong-yu, A·Yolwas, DAI Kang, SHEN Yi-fan* . Transfer Energy Disposal in Collisions of NaK(61Σ+) with H2 [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2014, 34(04): 884-887. |
[9] |
LIU Xiao-dong, ZHANG Fu-jun*, XU Zheng, ZHAO Su-ling, SONG Jing-lu, LI Jun-ming, SONG Dan-dan, WANG Yong-sheng. Influence of the Active Layer Thickness on the Performance of Bulk Heterojunction Solar Cell [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2010, 30(07): 1752-1755. |
[10] |
WANG Min,ZHOU Dong-dong,WANG Xue-yan,MU Nisai·NU,DAI Kang,WANG Qian,SHEN Yi-fan* . Collisional Excitation Transfer between Cs2(B 1Πu) and Cs(6S) [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2009, 29(11): 2889-2892. |
[11] |
ZHOU Jia-hong1, FENG Yu-ying1, WU Xiao-hong2,YANG Hui2, XING Wei3, LU Tian-hong2, 3* . Study on Interaction between CT-DNA and Cytochrome C Using UV-Vis Spectroscopy and Electrochemistry [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2005, 25(08): 1306-1308. |
[12] |
LEI Zhen-zhou, LIU Jing , ZHOU Xiao-yan , DAI Kang, SHEN Yi-fan* . Collisional Excitation Transfer between Na2(B 1Πu) and Na2(2 1Σ+g) [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2005, 25(02): 171-173. |
[13] |
LIU Jing, LEI Zhen-zhou, ZHOU Xiao-yan, DAI Kang, SHEN Yi-fan*. Photolytic Spectroscopy of Cs2 Molecules[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2004, 24(11): 1299-1301. |
[14] |
LIU Wei,YE Tao,GUO Rong. Study on the Reaction between Cobalt Tetrasulphonated Phthalocyanine and Sodium Sulfide[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2004, 24(06): 762-767. |
[15] |
WANG Xiao-cong1,2,SU Xiao-qiong2,ZHONG Qi-ling2*,HUANG Xi-gen2,REN Bin3,XIANG Juan3,TIAN Zhong-qun3 . In Situ Raman Spectroscopic Study on the Oxidation of HCHO on a Roughened Platinum Electrode [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2004, 24(03): 299-302. |
|
|
|
|