光谱学与光谱分析 |
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New Fluorescent Staining Method to Distinguish Normal and Malignant Rectum Tissues Using Terbium (Ⅲ)-Ciprofloxacin Complex |
MENG Tao1,3,XU Duan-fu1,ZHAO Ying1,WANG Du-jin1,XU Yi-zhuang2*,QI Jian2,WU Jin-guang2 |
1. State Key Laboratory of Polymer Physics and Chemistry, Joint Laboratory of Polymer Science and Materials, Institute of Chemistry, the Chinese Academy of Sciences, Beijing 100080, China 2.College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China 3. Beijing Institute of Graphic Communication, Beijing 102600,China |
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Abstract A new fluorescent staining method to distinguish normal and malignant rectum tissues using terbium(Ⅲ)-Ciprofloxacin complex was investigated. The terbium (Ⅲ) complex exhibits strong fluorescence and the staining process with the complex is very fast. It needs 3-5 minutes. The results showed that the fluorescence microscope images were similar to the images stained using traditional hematoxylin and eosin (H&E) methodology. The fluorescence spectra and FTIR spectra of normal and malignant rectum tissues were collected and the detection criterions for the malignant rectum tissues were the same as the staining results. In summary, the fluorescence staining may be developed as a simple and convenient method.
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Received: 2006-08-10
Accepted: 2006-12-20
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Corresponding Authors:
XU Yi-zhuang
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Cite this article: |
MENG Tao,XU Duan-fu,ZHAO Ying, et al. New Fluorescent Staining Method to Distinguish Normal and Malignant Rectum Tissues Using Terbium (Ⅲ)-Ciprofloxacin Complex [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2007, 27(10): 2069-2072.
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URL: |
https://www.gpxygpfx.com/EN/Y2007/V27/I10/2069 |
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